Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   PPM45_RS12605 Genome accession   NZ_CP116870
Coordinates   2463266..2463676 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis subsp. subtilis strain Master_strain     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2458266..2468676
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PPM45_RS12575 - 2458353..2458493 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  PPM45_RS12580 yqeF 2458799..2459530 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  PPM45_RS12585 cwlH 2459782..2460534 (-) 753 WP_003229963.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  PPM45_RS12590 yqeD 2460721..2461347 (+) 627 WP_003229962.1 TVP38/TMEM64 family protein -
  PPM45_RS12595 gnd 2461366..2462259 (-) 894 WP_003229961.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  PPM45_RS12600 yqeB 2462511..2463233 (+) 723 WP_010886572.1 YqeB family protein -
  PPM45_RS12605 nucA/comI 2463266..2463676 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  PPM45_RS12610 - 2463872..2464222 (+) 351 Protein_2434 sigma-70 family RNA polymerase sigma factor -
  PPM45_RS12615 spoIVCA 2464250..2465710 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  PPM45_RS12620 - 2465668..2465846 (-) 179 Protein_2436 hypothetical protein -
  PPM45_RS12625 arsC 2466201..2466620 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  PPM45_RS12630 - 2466632..2466760 (-) 129 Protein_2438 arsenical-resistance protein -
  PPM45_RS12635 - 2466758..2467144 (+) 387 Protein_2439 ImmA/IrrE family metallo-endopeptidase -
  PPM45_RS12640 - 2467144..2467542 (+) 399 Protein_2440 sigma-70 family RNA polymerase sigma factor -
  PPM45_RS12645 - 2467542..2467658 (+) 117 WP_003229898.1 hypothetical protein -
  PPM45_RS12650 - 2467624..2467824 (-) 201 Protein_2442 recombinase family protein -
  PPM45_RS12655 - 2467767..2467931 (-) 165 WP_010886576.1 hypothetical protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=780728 PPM45_RS12605 WP_009967785.1 2463266..2463676(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain Master_strain]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=780728 PPM45_RS12605 WP_009967785.1 2463266..2463676(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain Master_strain]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529