Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   PPM34_RS11950 Genome accession   NZ_CP116869
Coordinates   2335115..2335525 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis subsp. subtilis strain MGP001     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2330115..2340525
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PPM34_RS11920 - 2330202..2330342 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  PPM34_RS11925 yqeF 2330648..2331379 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  PPM34_RS11930 cwlH 2331631..2332383 (-) 753 WP_003229963.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  PPM34_RS11935 yqeD 2332570..2333196 (+) 627 WP_003229962.1 TVP38/TMEM64 family protein -
  PPM34_RS11940 gnd 2333215..2334108 (-) 894 WP_003229961.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  PPM34_RS11945 yqeB 2334360..2335082 (+) 723 WP_010886572.1 YqeB family protein -
  PPM34_RS11950 nucA/comI 2335115..2335525 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  PPM34_RS11955 - 2335721..2336071 (+) 351 Protein_2303 sigma-70 family RNA polymerase sigma factor -
  PPM34_RS11960 spoIVCA 2336099..2337559 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  PPM34_RS11965 - 2337517..2337695 (-) 179 Protein_2305 hypothetical protein -
  PPM34_RS11970 arsC 2338050..2338469 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  PPM34_RS11975 - 2338481..2338609 (-) 129 Protein_2307 arsenical-resistance protein -
  PPM34_RS11980 - 2338607..2338993 (+) 387 Protein_2308 ImmA/IrrE family metallo-endopeptidase -
  PPM34_RS11985 - 2338993..2339391 (+) 399 Protein_2309 sigma-70 family RNA polymerase sigma factor -
  PPM34_RS11990 - 2339391..2339507 (+) 117 WP_003229898.1 hypothetical protein -
  PPM34_RS11995 - 2339473..2339673 (-) 201 Protein_2311 recombinase family protein -
  PPM34_RS12000 - 2339616..2339780 (-) 165 WP_010886576.1 hypothetical protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=780643 PPM34_RS11950 WP_009967785.1 2335115..2335525(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain MGP001]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=780643 PPM34_RS11950 WP_009967785.1 2335115..2335525(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain MGP001]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529