Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   PPM66_RS11780 Genome accession   NZ_CP116867
Coordinates   2246572..2246982 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis subsp. subtilis strain MGP008     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2241572..2251982
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PPM66_RS11750 - 2241659..2241799 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  PPM66_RS11755 yqeF 2242105..2242836 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  PPM66_RS11760 cwlH 2243088..2243840 (-) 753 WP_003229963.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  PPM66_RS11765 yqeD 2244027..2244653 (+) 627 WP_003229962.1 TVP38/TMEM64 family protein -
  PPM66_RS11770 gnd 2244672..2245565 (-) 894 WP_003229961.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  PPM66_RS11775 yqeB 2245817..2246539 (+) 723 WP_010886572.1 YqeB family protein -
  PPM66_RS11780 nucA/comI 2246572..2246982 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  PPM66_RS11785 - 2247178..2247528 (+) 351 Protein_2268 sigma-70 family RNA polymerase sigma factor -
  PPM66_RS11790 spoIVCA 2247556..2249016 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  PPM66_RS11795 - 2248974..2249152 (-) 179 Protein_2270 hypothetical protein -
  PPM66_RS11800 arsC 2249507..2249926 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  PPM66_RS11805 - 2249938..2250066 (-) 129 Protein_2272 arsenical-resistance protein -
  PPM66_RS11810 - 2250064..2250450 (+) 387 Protein_2273 ImmA/IrrE family metallo-endopeptidase -
  PPM66_RS11815 - 2250450..2250848 (+) 399 Protein_2274 sigma-70 family RNA polymerase sigma factor -
  PPM66_RS11820 - 2250848..2250964 (+) 117 WP_003229898.1 hypothetical protein -
  PPM66_RS11825 - 2250930..2251130 (-) 201 Protein_2276 recombinase family protein -
  PPM66_RS11830 - 2251073..2251237 (-) 165 WP_010886576.1 hypothetical protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=780475 PPM66_RS11780 WP_009967785.1 2246572..2246982(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain MGP008]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=780475 PPM66_RS11780 WP_009967785.1 2246572..2246982(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain MGP008]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529