Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   PPM66_RS01335 Genome accession   NZ_CP116867
Coordinates   241479..241928 (-) Length   149 a.a.
NCBI ID   WP_003246436.1    Uniprot ID   A0AAE2SFQ0
Organism   Bacillus subtilis subsp. subtilis strain MGP008     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 236479..246928
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PPM66_RS01300 yckA 236630..237310 (-) 681 WP_003234624.1 amino acid ABC transporter permease -
  PPM66_RS01305 yckB 237320..238183 (-) 864 WP_009966510.1 transporter substrate-binding domain-containing protein -
  PPM66_RS01310 - 238345..238542 (+) 198 WP_119122841.1 hypothetical protein -
  PPM66_RS01315 yckC 238561..239016 (+) 456 WP_003246356.1 RDD family protein -
  PPM66_RS01320 yckD 239098..239430 (+) 333 WP_003246247.1 YckD family protein -
  PPM66_RS01325 bglC 239584..241017 (+) 1434 WP_003246242.1 6-phospho-beta-glucosidase -
  PPM66_RS01330 nin/comJ 241054..241452 (-) 399 WP_003246342.1 DNA-entry nuclease inhibitor Regulator
  PPM66_RS01335 nucA/comI 241479..241928 (-) 450 WP_003246436.1 DNA-entry nuclease Machinery gene
  PPM66_RS01340 tlpC 242096..243817 (-) 1722 WP_003246498.1 methyl-accepting chemotaxis protein TlpC -
  PPM66_RS01345 hxlB 243928..244485 (-) 558 WP_003246343.1 6-phospho-3-hexuloisomerase -
  PPM66_RS01350 hxlA 244491..245123 (-) 633 WP_003246452.1 3-hexulose-6-phosphate synthase -
  PPM66_RS01355 hxlR 245357..245719 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16486.48 Da        Isoelectric Point: 4.6274

>NTDB_id=780425 PPM66_RS01335 WP_003246436.1 241479..241928(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain MGP008]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETMAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHRLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=780425 PPM66_RS01335 WP_003246436.1 241479..241928(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain MGP008]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATGGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAAGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
GGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(53-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

100

100

1