Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   PNF30_RS10380 Genome accession   NZ_CP116774
Coordinates   2009561..2009941 (-) Length   126 a.a.
NCBI ID   WP_223250982.1    Uniprot ID   -
Organism   Bacillus safensis strain SRCM125915     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2004561..2014941
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PNF30_RS10345 (PNF30_10345) - 2005152..2005382 (-) 231 WP_048240230.1 DUF896 domain-containing protein -
  PNF30_RS10350 (PNF30_10350) - 2005452..2006099 (-) 648 WP_073979991.1 YneB family resolvase-like protein -
  PNF30_RS10355 (PNF30_10355) yneA 2006117..2006428 (-) 312 WP_024425130.1 cell division suppressor protein YneA -
  PNF30_RS10360 (PNF30_10360) lexA 2006588..2007208 (+) 621 WP_034283422.1 transcriptional repressor LexA -
  PNF30_RS10365 (PNF30_10365) - 2007256..2007594 (-) 339 WP_272510119.1 hypothetical protein -
  PNF30_RS10370 (PNF30_10370) - 2007992..2008534 (+) 543 WP_095408609.1 IseA DL-endopeptidase inhibitor family protein -
  PNF30_RS10375 (PNF30_10375) - 2008566..2009414 (-) 849 WP_111292992.1 STAS domain-containing protein -
  PNF30_RS10380 (PNF30_10380) nucA/comI 2009561..2009941 (-) 381 WP_223250982.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  PNF30_RS10385 (PNF30_10385) - 2010174..2011082 (-) 909 WP_039178896.1 trypsin-like serine peptidase -
  PNF30_RS10390 (PNF30_10390) - 2011277..2014420 (-) 3144 WP_272510120.1 bifunctional cytochrome P450/NADPH--P450 reductase -

Sequence

Protein


Download         Length: 126 a.a.        Molecular weight: 13713.38 Da        Isoelectric Point: 8.5068

>NTDB_id=780181 PNF30_RS10380 WP_223250982.1 2009561..2009941(-) (nucA/comI) [Bacillus safensis strain SRCM125915]
MGTLLGGFGEKQAAKGADRYDHVVQFPKERYPETGSHIQDAIRKGHSDVCTIDRNGADARRQESLKGIPTKPGFDRDEWP
MAVCLEGGKGASVQYVSPSDNRGAGSWVGHQISGFPDGKRILFIVK

Nucleotide


Download         Length: 381 bp        

>NTDB_id=780181 PNF30_RS10380 WP_223250982.1 2009561..2009941(-) (nucA/comI) [Bacillus safensis strain SRCM125915]
ATGGGCACGTTGTTAGGTGGATTTGGAGAGAAGCAGGCAGCAAAAGGTGCTGATCGATATGATCATGTCGTTCAATTTCC
AAAGGAACGATACCCTGAAACAGGCAGTCATATTCAAGATGCCATTCGAAAAGGGCATTCAGATGTGTGTACCATTGACC
GAAATGGAGCAGATGCCCGCAGGCAAGAATCATTAAAAGGAATTCCGACAAAACCTGGCTTTGACCGGGATGAATGGCCG
ATGGCGGTTTGTCTTGAGGGAGGAAAAGGCGCAAGCGTTCAATATGTCAGTCCATCTGATAATAGGGGAGCAGGCTCATG
GGTCGGCCATCAAATCAGTGGGTTTCCTGATGGAAAAAGAATATTATTCATTGTGAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(29-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

58.824

94.444

0.556