Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   PNF30_RS07415 Genome accession   NZ_CP116774
Coordinates   1456735..1457505 (+) Length   256 a.a.
NCBI ID   WP_095409043.1    Uniprot ID   -
Organism   Bacillus safensis strain SRCM125915     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 1451735..1462505
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PNF30_RS07385 (PNF30_07385) - 1452042..1454153 (+) 2112 WP_391509730.1 HD family phosphohydrolase -
  PNF30_RS07390 (PNF30_07390) ybeY 1454154..1454633 (+) 480 WP_272510901.1 rRNA maturation RNase YbeY -
  PNF30_RS07395 (PNF30_07395) - 1454611..1454997 (+) 387 WP_024423020.1 diacylglycerol kinase family protein -
  PNF30_RS07400 (PNF30_07400) - 1455111..1455509 (+) 399 WP_008356263.1 cytidine deaminase -
  PNF30_RS07405 (PNF30_07405) era 1455502..1456407 (+) 906 WP_007501305.1 GTPase Era -
  PNF30_RS07410 (PNF30_07410) - 1456556..1456699 (+) 144 WP_003217288.1 YqzL family protein -
  PNF30_RS07415 (PNF30_07415) recO 1456735..1457505 (+) 771 WP_095409043.1 DNA repair protein RecO Machinery gene
  PNF30_RS07420 (PNF30_07420) glyQ 1457834..1458721 (+) 888 WP_272510902.1 glycine--tRNA ligase subunit alpha -
  PNF30_RS07425 (PNF30_07425) glyS 1458714..1460759 (+) 2046 WP_272510903.1 glycine--tRNA ligase subunit beta -
  PNF30_RS07430 (PNF30_07430) - 1460927..1461565 (+) 639 WP_024423017.1 helix-turn-helix transcriptional regulator -
  PNF30_RS07435 (PNF30_07435) - 1461591..1462412 (+) 822 WP_008342399.1 pyruvate, water dikinase regulatory protein -

Sequence

Protein


Download         Length: 256 a.a.        Molecular weight: 29288.93 Da        Isoelectric Point: 8.1204

>NTDB_id=780174 PNF30_RS07415 WP_095409043.1 1456735..1457505(+) (recO) [Bacillus safensis strain SRCM125915]
MLIKSEGIVLRTTDYGETNKIVTLLTREHGKIGVMARGAKKSNSRLSAISQPFLYGTFLIQSSTGLGTLQQGEMIESMRT
IREDLFLTAYAAYMTELLDKGTEEKKPNPYLFELLLQSLRHLNEGTDADIILFIVEVKMLSVMGMKPELDQCVHCGQKEG
QFHFSIRDNGFICQNCFSKDPYKLPLSPAAARLLRLFHYFDLSRLGQVDVKPQTKQEIRQVLDHYYDEYSGLYLKSKKFM
NQMESMKKLMGDENKS

Nucleotide


Download         Length: 771 bp        

>NTDB_id=780174 PNF30_RS07415 WP_095409043.1 1456735..1457505(+) (recO) [Bacillus safensis strain SRCM125915]
ATGCTCATTAAAAGTGAAGGAATCGTCCTTAGAACAACAGATTATGGCGAAACAAACAAAATTGTCACGCTGCTCACTAG
AGAACATGGAAAAATTGGCGTGATGGCAAGAGGCGCTAAAAAATCAAACAGCCGCCTGTCAGCTATAAGCCAGCCGTTTT
TATACGGCACATTTCTCATCCAATCATCAACAGGACTTGGAACGCTCCAGCAAGGCGAAATGATTGAAAGTATGCGGACC
ATTCGGGAAGACTTATTTCTGACCGCTTATGCTGCATATATGACGGAATTACTTGATAAAGGAACAGAAGAAAAAAAGCC
AAACCCTTATTTGTTTGAGCTTCTGCTTCAATCTCTGCGTCATCTCAATGAAGGCACAGATGCTGATATTATTTTATTTA
TCGTAGAGGTCAAAATGTTATCAGTGATGGGGATGAAGCCTGAACTGGATCAATGTGTCCACTGTGGTCAAAAAGAAGGG
CAGTTCCATTTCTCGATAAGAGATAACGGATTCATTTGCCAAAATTGTTTTTCGAAAGATCCATATAAACTGCCCTTATC
CCCTGCAGCTGCAAGGCTGCTCCGTTTGTTTCATTATTTTGATTTATCAAGGCTTGGTCAGGTCGATGTGAAGCCGCAGA
CGAAACAAGAAATTCGTCAAGTGCTTGATCATTACTATGACGAATATTCCGGCCTCTATTTAAAATCAAAAAAATTCATG
AATCAGATGGAATCCATGAAAAAGCTAATGGGTGACGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-77)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

75.391

100

0.754