Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   PNF29_RS08945 Genome accession   NZ_CP116773
Coordinates   1697147..1698217 (+) Length   356 a.a.
NCBI ID   WP_004399124.1    Uniprot ID   A0A809FNW6
Organism   Bacillus subtilis strain SRCM125727     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1692147..1703217
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PNF29_RS08910 (PNF29_08910) yqgY 1692343..1692588 (-) 246 WP_041334980.1 DUF2626 domain-containing protein -
  PNF29_RS08915 (PNF29_08915) mgsR 1692820..1693200 (+) 381 WP_003230147.1 transcriptional regulator MgsR -
  PNF29_RS08925 (PNF29_08925) rsbRD 1693424..1694260 (+) 837 WP_017696187.1 RsbT co-antagonist protein RsbRD -
  PNF29_RS08930 (PNF29_08930) yqhB 1694313..1695641 (-) 1329 WP_017696188.1 hemolysin family protein -
  PNF29_RS08935 (PNF29_08935) corA 1695783..1696736 (+) 954 WP_272516111.1 magnesium transporter CorA -
  PNF29_RS08940 (PNF29_08940) - 1696738..1696935 (+) 198 WP_272516202.1 CBS domain-containing protein -
  PNF29_RS08945 (PNF29_08945) comGA 1697147..1698217 (+) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  PNF29_RS08950 (PNF29_08950) comGB 1698204..1699241 (+) 1038 WP_017696193.1 competence type IV pilus assembly protein ComGB Machinery gene
  PNF29_RS08955 (PNF29_08955) comGC 1699255..1699551 (+) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  PNF29_RS08960 (PNF29_08960) comGD 1699541..1699972 (+) 432 WP_017696194.1 competence type IV pilus minor pilin ComGD Machinery gene
  PNF29_RS08965 (PNF29_08965) comGE 1699956..1700303 (+) 348 WP_017696195.1 competence type IV pilus minor pilin ComGE Machinery gene
  PNF29_RS08970 (PNF29_08970) comGF 1700329..1700712 (+) 384 WP_017696196.1 ComG operon protein ComGF Machinery gene
  PNF29_RS08975 (PNF29_08975) comGG 1700713..1701087 (+) 375 WP_017696197.1 ComG operon protein ComGG Machinery gene
  PNF29_RS08980 (PNF29_08980) spoIITA 1701158..1701337 (+) 180 WP_014480252.1 YqzE family protein -
  PNF29_RS08985 (PNF29_08985) yqzG 1701379..1701705 (-) 327 WP_026113671.1 YqzG/YhdC family protein -
  PNF29_RS08990 (PNF29_08990) tapA 1701976..1702737 (+) 762 WP_029317916.1 amyloid fiber anchoring/assembly protein TapA -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40458.64 Da        Isoelectric Point: 9.0175

>NTDB_id=780103 PNF29_RS08945 WP_004399124.1 1697147..1698217(+) (comGA) [Bacillus subtilis strain SRCM125727]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=780103 PNF29_RS08945 WP_004399124.1 1697147..1698217(+) (comGA) [Bacillus subtilis strain SRCM125727]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAAGCATATCTAACAAAGGCTTCTGATATTCACATTGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGGCTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGCTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCAACTGGTTCAGGGAAGACTACCACACTATACTCTCTCGTTCAATAC
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAAGCAATTTTGCGCCATGACCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTAACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATTGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGCCGACAGTCAC
GAAATACCAGGAGAGCTAGCGTTTATGAGCTTCTTTATGGGAAAAACCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGAAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A809FNW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

100

100

1