Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   PNF29_RS08665 Genome accession   NZ_CP116773
Coordinates   1647263..1648030 (+) Length   255 a.a.
NCBI ID   WP_017696274.1    Uniprot ID   -
Organism   Bacillus subtilis strain SRCM125727     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 1642263..1653030
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PNF29_RS08635 (PNF29_08635) pgpH 1642559..1644694 (+) 2136 WP_272516107.1 cyclic-di-AMP phosphodiesterase PgpH -
  PNF29_RS08640 (PNF29_08640) ybeY 1644695..1645168 (+) 474 WP_041333780.1 rRNA maturation RNase YbeY -
  PNF29_RS08645 (PNF29_08645) unpK 1645149..1645520 (+) 372 WP_041333781.1 diacylglycerol kinase family protein -
  PNF29_RS08650 (PNF29_08650) - 1645639..1646049 (+) 411 WP_014480293.1 cytidine deaminase -
  PNF29_RS08655 (PNF29_08655) era 1646030..1646935 (+) 906 WP_003230051.1 GTPase Era -
  PNF29_RS08660 (PNF29_08660) yqzL 1647083..1647226 (+) 144 WP_003230053.1 YqzL family protein -
  PNF29_RS08665 (PNF29_08665) recO 1647263..1648030 (+) 768 WP_017696274.1 DNA repair protein RecO Machinery gene
  PNF29_RS08670 (PNF29_08670) glyQ 1648327..1649214 (+) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  PNF29_RS08675 (PNF29_08675) glyS 1649207..1651246 (+) 2040 WP_014480291.1 glycine--tRNA ligase subunit beta -
  PNF29_RS08680 (PNF29_08680) ccpN 1651379..1652017 (+) 639 WP_003237058.1 transcriptional regulator CcpN -
  PNF29_RS08685 (PNF29_08685) ppsR 1652043..1652855 (+) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29346.03 Da        Isoelectric Point: 8.3080

>NTDB_id=780102 PNF29_RS08665 WP_017696274.1 1647263..1648030(+) (recO) [Bacillus subtilis strain SRCM125727]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYIAELVDRGTEEKKTNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=780102 PNF29_RS08665 WP_017696274.1 1647263..1648030(+) (recO) [Bacillus subtilis strain SRCM125727]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACTTTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATATTGCTGAGTTAGTCGACAGAGGCACAGAAGAAAAAAAGAC
AAATCCTTATTTATTTGAATTCATATTAGAGTCATTAAAACAGCTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTAAATCATTGTGTACATTGTAAAAGCCAAGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGATTAA
ACCGCAAACGGCAAGGCTGTTAAGACTCTTTTACTATTTTGATCTGTCGAGGCTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

99.216

100

0.992