Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MWLf4_RS09465 Genome accession   NZ_CP116755
Coordinates   1840260..1841633 (-) Length   457 a.a.
NCBI ID   WP_272537088.1    Uniprot ID   -
Organism   Limosilactobacillus fermentum strain MWLf-4     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1835260..1846633
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWLf4_RS09450 (MWLf4_1925) - 1836890..1837459 (-) 570 WP_004562737.1 TIGR01440 family protein -
  MWLf4_RS09455 (MWLf4_1926) gltX 1837527..1839023 (-) 1497 WP_012390831.1 glutamate--tRNA ligase -
  MWLf4_RS09460 (MWLf4_1927) - 1839094..1840227 (-) 1134 WP_004562735.1 PIN/TRAM domain-containing protein -
  MWLf4_RS09465 (MWLf4_1928) radA 1840260..1841633 (-) 1374 WP_272537088.1 DNA repair protein RadA Machinery gene
  MWLf4_RS09470 (MWLf4_1929) - 1841646..1842185 (-) 540 WP_004563338.1 dUTP diphosphatase -
  MWLf4_RS09475 (MWLf4_1930) - 1842385..1842696 (+) 312 WP_004562732.1 GNAT family N-acetyltransferase -
  MWLf4_RS09480 (MWLf4_1931) rpiA 1842708..1843394 (+) 687 WP_014562151.1 ribose-5-phosphate isomerase RpiA -
  MWLf4_RS09485 (MWLf4_1932) - 1843422..1844036 (+) 615 WP_004563340.1 sugar O-acetyltransferase -
  MWLf4_RS09490 (MWLf4_1933) - 1844100..1845437 (+) 1338 WP_014562150.1 aminopeptidase C -
  MWLf4_RS09495 (MWLf4_1934) - 1845754..1846629 (-) 876 WP_004562728.1 alpha/beta hydrolase -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49377.80 Da        Isoelectric Point: 8.7358

>NTDB_id=779978 MWLf4_RS09465 WP_272537088.1 1840260..1841633(-) (radA) [Limosilactobacillus fermentum strain MWLf-4]
MAKAKTHFVCQNCGYNSPRYLGRCPNCGQWSTLVEEVEQASTPAAKNATATLTGIVARPQRIQEIDTKKTPRVKTRLNEL
NRVLGGGIVPGSLVLIGGDPGIGKSTLLLQVSGQLSVEHHKVLYVSGEESASQIKLRAQRLDVSGEDFYIYPETNMEAIR
ATIDSIQPEFVIIDSVQTMQAADVTSAIGSVSQIRAVTAQLMQIAKGQNITVFVVGHVTKGGVLAGPKILEHMVDTVLYF
EGDLHHTYRILRSVKNRFGSTNELGIFEMNTRGLTEVANPSEIFLEERLKDATGSAVVVSLEGTRPILVEIQALITPTVF
GNAQRTATGLNRNRVSLIMAVLEKRANLLLQNQDAYLKAAGGVKLDEPAIDLAIAVAIASSYRDKGTRPTDAFVGEVGLT
GEIRRVSRIEQRVAEAAKLGFKRILVPKNNLAGWNPPQDIEVVGVTPLGEALKIALG

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=779978 MWLf4_RS09465 WP_272537088.1 1840260..1841633(-) (radA) [Limosilactobacillus fermentum strain MWLf-4]
ATGGCAAAAGCCAAAACACACTTTGTCTGCCAAAACTGCGGTTACAATTCACCGCGCTATTTAGGGCGGTGTCCCAATTG
TGGCCAGTGGAGTACCTTAGTTGAAGAAGTCGAGCAGGCAAGTACCCCGGCGGCTAAAAACGCTACGGCAACCTTAACCG
GGATTGTGGCCCGGCCCCAGCGAATCCAAGAAATTGACACCAAAAAAACGCCCCGGGTTAAGACCCGCTTAAATGAGTTA
AACCGGGTGCTAGGTGGCGGAATTGTACCCGGCTCGTTGGTCTTAATTGGTGGGGATCCCGGGATCGGAAAGTCCACCCT
GCTCTTACAAGTGTCGGGGCAACTGAGCGTGGAACACCACAAGGTTCTTTACGTTTCCGGGGAAGAATCGGCCTCCCAAA
TTAAATTGCGGGCCCAACGCTTAGACGTTTCCGGCGAGGACTTTTATATCTACCCGGAAACCAACATGGAAGCCATCCGG
GCGACGATTGATTCCATTCAGCCCGAGTTTGTAATCATTGACTCGGTTCAGACGATGCAGGCGGCCGACGTGACCTCGGC
GATTGGGTCGGTCTCCCAAATCCGGGCGGTCACCGCCCAGTTGATGCAGATTGCCAAGGGGCAAAACATCACGGTCTTCG
TGGTTGGTCACGTCACCAAGGGTGGGGTCTTAGCCGGGCCCAAGATCTTGGAACACATGGTTGACACGGTGCTGTACTTT
GAAGGGGACCTCCACCACACCTACCGGATTTTACGGTCGGTCAAAAACCGGTTTGGGTCCACCAATGAGTTAGGGATCTT
CGAAATGAATACCCGCGGTTTGACCGAGGTCGCAAACCCCTCGGAAATCTTTTTGGAAGAGCGGCTTAAGGATGCCACCG
GGTCGGCCGTGGTCGTATCCCTGGAGGGAACCCGACCGATCCTGGTTGAAATCCAGGCCTTGATCACGCCAACCGTCTTT
GGAAACGCCCAAAGAACGGCGACAGGTTTAAACCGCAACCGGGTTTCCTTGATCATGGCGGTCCTAGAAAAACGGGCGAA
CCTGCTCTTACAAAACCAGGATGCCTACTTGAAGGCGGCCGGGGGCGTTAAGTTAGACGAGCCGGCCATCGACCTTGCGA
TCGCGGTGGCAATTGCTTCTTCTTACCGGGATAAGGGGACCCGGCCGACCGACGCCTTCGTCGGTGAAGTTGGCTTAACC
GGGGAGATCCGTCGGGTGAGCCGAATTGAACAGCGGGTGGCCGAAGCCGCTAAACTAGGCTTTAAGCGGATTTTAGTGCC
CAAAAACAATTTAGCGGGTTGGAACCCGCCGCAAGACATCGAAGTCGTGGGGGTAACGCCCCTTGGCGAAGCACTTAAAA
TTGCCCTTGGATAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)

AAA domain (AAA_25)

(64-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

67.991

99.125

0.674
  radA Streptococcus mitis NCTC 12261

67.77

99.125

0.672
  radA Streptococcus pneumoniae Rx1

67.77

99.125

0.672
  radA Streptococcus pneumoniae D39

67.77

99.125

0.672
  radA Streptococcus pneumoniae R6

67.77

99.125

0.672
  radA Streptococcus pneumoniae TIGR4

67.77

99.125

0.672
  radA Bacillus subtilis subsp. subtilis str. 168

63.676

100

0.637