Detailed information    

insolico Bioinformatically predicted

Overview

Name   clpE   Type   Regulator
Locus tag   POF58_RS02995 Genome accession   NZ_CP116711
Coordinates   534305..536566 (-) Length   753 a.a.
NCBI ID   WP_000882547.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain CNCTC 10/84 Cas9 AEKOrevertant     
Function   repress competence development (at the early growth phase) (predicted from homology)   
Competence regulation

Genomic Context

Location: 529305..541566
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  POF58_RS02975 (POF58_02975) - 529334..530104 (+) 771 WP_001131467.1 DivIVA domain-containing protein -
  POF58_RS02980 (POF58_02980) ileS 530389..533181 (+) 2793 WP_000768156.1 isoleucine--tRNA ligase -
  POF58_RS02985 (POF58_02985) - 533298..533600 (-) 303 WP_001237035.1 DUF1827 family protein -
  POF58_RS02990 (POF58_02990) - 533664..534119 (-) 456 WP_000184290.1 NUDIX hydrolase -
  POF58_RS02995 (POF58_02995) clpE 534305..536566 (-) 2262 WP_000882547.1 ATP-dependent Clp protease ATP-binding subunit Regulator
  POF58_RS03000 (POF58_03000) - 536864..537094 (+) 231 WP_000443582.1 DUF1797 family protein -
  POF58_RS03005 (POF58_03005) - 537235..537927 (+) 693 WP_001011647.1 amino acid ABC transporter permease -
  POF58_RS03010 (POF58_03010) - 537920..538654 (+) 735 WP_000230128.1 amino acid ABC transporter ATP-binding protein -
  POF58_RS03015 (POF58_03015) - 538940..540634 (+) 1695 WP_001106851.1 phospho-sugar mutase -

Sequence

Protein


Download         Length: 753 a.a.        Molecular weight: 83776.96 Da        Isoelectric Point: 5.2771

>NTDB_id=779467 POF58_RS02995 WP_000882547.1 534305..536566(-) (clpE) [Streptococcus agalactiae strain CNCTC 10/84 Cas9 AEKOrevertant]
MLCQNCKLNESTIHLYTNVNGKQKQVDLCQNCYQIIKTDPNNPLFSGLNHVSHAPGGINPFFDDFFGDLNNFRAFNGQDL
PNTPPTQSGGNRGGGNGNGRNNNRNQTATPPQAKGILEEFGINVTEIARHGDIDPVIGRDSEIIRVIEILNRRTKNNPVL
IGEPGVGKTAVVEGLAQKIVDGNVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEIRQRQDVILFIDEIHEIVG
AGTAGEGSMDAGNILKPALARGELQLVGATTLNEYRIIEKDAALERRMQPVKVDEPSVEETITILKGIQKKYEDYHHVKY
NNDAIEAAAVLSNRYIQDRFLPDKAIDLLDEAGSKMNLTLNFVDPKEIDQRLIEAENLKAQATREEDYERAAYFRDQIAK
YKEMQQQKVDDQDTPIITEKTIEHIIEEKTNIPVGDLKEKEQSQLINLADDLKQHVIGQDDAVVKIAKAIRRNRVGLGSP
NRPIGSFLFVGPTGVGKTELSKQLAIELFGSADSMIRFDMSEYMEKHAVAKLVGAPPGYVGYEEAGQLTEKVRRNPYSLI
LLDEIEKAHPDVMHMFLQVLDDGRLTDGQGRTVSFKDTIIIMTSNAGSGKTEASVGFGASREGRTNSVLGQLGNFFSPEF
MNRFDGIIEFKALDKENLLNIVDIMLSDVNARLAINGIHLDVTDKVKEKLVDLGYDPKMGARPLRRTIQEHIEDAITDYY
LENPSEKELRAIMTSNGNIIIKSSKKTEESTKG

Nucleotide


Download         Length: 2262 bp        

>NTDB_id=779467 POF58_RS02995 WP_000882547.1 534305..536566(-) (clpE) [Streptococcus agalactiae strain CNCTC 10/84 Cas9 AEKOrevertant]
ATGCTCTGTCAAAACTGCAAACTAAACGAATCAACTATTCATTTGTATACAAATGTTAATGGAAAACAAAAGCAAGTTGA
TCTTTGTCAAAATTGCTACCAAATTATTAAAACTGACCCTAATAATCCACTTTTTTCAGGTTTAAATCATGTTTCACATG
CGCCTGGTGGCATCAATCCTTTCTTTGATGATTTCTTTGGAGATTTAAATAATTTCAGAGCCTTTAATGGCCAAGATTTA
CCAAACACTCCTCCTACACAATCAGGAGGAAACCGAGGTGGAGGAAACGGTAATGGACGAAATAATAATCGCAACCAAAC
TGCAACTCCTCCTCAAGCCAAAGGGATTCTAGAAGAATTTGGTATCAATGTCACTGAAATAGCCCGCCACGGTGATATTG
ACCCTGTTATTGGACGTGACTCAGAAATTATTCGTGTTATCGAAATCCTTAATCGTCGTACAAAAAATAATCCTGTACTT
ATTGGCGAACCTGGCGTAGGGAAAACTGCCGTTGTTGAAGGACTTGCTCAGAAAATTGTTGATGGTAATGTGCCTCATAA
ACTTCAAGGCAAACAAGTTATTCGTTTGGATGTTGTGAGTCTAGTACAAGGAACGGGCATTCGTGGTCAATTTGAAGAAC
GCATGCAAAAGTTGATGGAAGAAATTCGTCAACGTCAAGATGTTATTCTATTCATAGATGAAATTCACGAAATAGTTGGA
GCTGGTACAGCTGGCGAAGGTAGTATGGATGCAGGTAATATTTTAAAACCTGCCCTTGCACGTGGAGAATTACAACTAGT
AGGTGCTACTACCCTCAATGAATATCGTATTATCGAAAAGGACGCTGCCTTAGAACGCCGTATGCAACCTGTAAAAGTCG
ATGAGCCTTCTGTTGAAGAAACCATTACTATTTTGAAAGGTATCCAAAAAAAATACGAAGATTATCATCACGTAAAATAT
AATAATGATGCCATAGAAGCAGCTGCAGTACTATCTAATCGTTATATCCAAGACCGCTTTTTACCTGATAAAGCAATAGA
CTTATTAGATGAAGCTGGTTCTAAAATGAACCTAACACTAAATTTTGTTGATCCAAAAGAAATTGATCAACGTCTCATTG
AAGCAGAAAATTTAAAAGCGCAAGCGACTCGTGAAGAAGATTACGAACGTGCAGCTTACTTCCGTGACCAGATTGCAAAA
TATAAAGAAATGCAACAACAAAAGGTCGACGATCAAGATACACCTATTATTACCGAAAAAACAATTGAGCACATCATTGA
AGAAAAAACGAATATCCCTGTTGGTGATTTAAAAGAAAAAGAACAATCTCAATTAATTAATCTCGCAGATGACTTGAAAC
AACATGTGATCGGCCAGGATGACGCTGTCGTTAAGATTGCAAAAGCTATTCGTCGTAATCGAGTTGGTCTTGGTAGCCCA
AACCGTCCTATTGGTTCCTTTTTATTTGTAGGACCAACCGGCGTTGGTAAAACTGAACTTTCTAAACAACTAGCAATTGA
GCTCTTTGGTTCAGCTGATAGTATGATTCGTTTTGATATGTCAGAGTACATGGAAAAGCATGCTGTTGCTAAATTAGTCG
GAGCGCCTCCAGGATACGTGGGATACGAGGAAGCTGGACAATTAACTGAAAAGGTTCGTCGAAATCCTTACTCGCTCATC
CTTCTAGATGAAATTGAAAAAGCTCATCCTGATGTCATGCATATGTTCTTGCAGGTCCTTGATGACGGTCGATTAACAGA
TGGACAAGGAAGAACTGTTAGTTTTAAAGATACCATTATCATCATGACCTCAAATGCTGGTTCTGGTAAAACTGAAGCAA
GCGTTGGCTTTGGTGCCTCACGAGAAGGTAGAACGAATTCCGTACTAGGTCAACTAGGTAACTTCTTCAGCCCTGAATTT
ATGAACCGCTTTGACGGTATTATTGAATTCAAGGCTTTAGATAAAGAGAATCTCCTCAATATCGTTGATATTATGTTATC
TGACGTTAATGCACGTCTCGCCATCAATGGTATTCATCTAGATGTCACTGATAAAGTGAAAGAAAAATTGGTTGATTTAG
GTTACGATCCAAAAATGGGAGCACGTCCATTACGTCGTACCATTCAAGAACATATTGAAGATGCTATCACAGATTACTAT
CTTGAGAATCCAAGCGAAAAAGAACTTCGTGCTATTATGACTAGCAATGGAAATATCATAATAAAATCTTCTAAAAAAAC
TGAAGAAAGTACAAAAGGTTGA

Domains

Predicted by InterproScan.

ATPase family associated with various cellular activities (AAA) (AAA)

(159-276)

AAA domain (Cdc48 subfamily) (AAA_2)

(482-645)

C-terminal, D2-small domain, of ClpB protein (ClpB_D2-small)

(653-727)

AAA lid domain (AAA_lid_9)

(297-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  clpE Streptococcus mutans UA159

85.676

100

0.858
  clpE Streptococcus pneumoniae Rx1

85.581

99.469

0.851
  clpE Streptococcus pneumoniae D39

85.581

99.469

0.851
  clpE Streptococcus pneumoniae R6

85.581

99.469

0.851
  clpE Streptococcus pneumoniae TIGR4

85.447

99.469

0.85
  clpC Lactococcus lactis subsp. cremoris KW2

74.867

99.867

0.748
  clpC Bacillus subtilis subsp. subtilis str. 168

51.327

90.04

0.462
  clpC Lactococcus lactis subsp. lactis strain DGCC12653

47.528

83.267

0.396
  clpC Streptococcus pneumoniae Rx1

47.003

84.197

0.396
  clpC Streptococcus pneumoniae D39

47.003

84.197

0.396
  clpC Streptococcus pneumoniae TIGR4

46.845

84.197

0.394
  clpC Streptococcus mutans UA159

47.03

82.736

0.389
  clpC Streptococcus thermophilus LMD-9

46.276

83.798

0.388
  clpC Streptococcus thermophilus LMG 18311

45.959

83.798

0.385