Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PHA77_RS14150 Genome accession   NZ_CP116675
Coordinates   3059105..3060574 (+) Length   489 a.a.
NCBI ID   WP_005281701.1    Uniprot ID   D4F169
Organism   Edwardsiella tarda strain SC002     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3054105..3065574
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PHA77_RS14120 (PHA77_14120) - 3054535..3055524 (+) 990 WP_005281708.1 calcium/sodium antiporter -
  PHA77_RS14125 (PHA77_14125) kdsD 3055577..3056563 (+) 987 WP_005281707.1 arabinose-5-phosphate isomerase KdsD -
  PHA77_RS14130 (PHA77_14130) kdsC 3056579..3057145 (+) 567 WP_005297337.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  PHA77_RS14135 (PHA77_14135) lptC 3057142..3057714 (+) 573 WP_005281705.1 LPS export ABC transporter periplasmic protein LptC -
  PHA77_RS14140 (PHA77_14140) lptA 3057689..3058246 (+) 558 WP_005281704.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  PHA77_RS14145 (PHA77_14145) lptB 3058253..3058978 (+) 726 WP_005281703.1 LPS export ABC transporter ATP-binding protein -
  PHA77_RS14150 (PHA77_14150) rpoN/rpoN1 3059105..3060574 (+) 1470 WP_005281701.1 RNA polymerase factor sigma-54 Machinery gene
  PHA77_RS14155 (PHA77_14155) hpf 3060596..3060883 (+) 288 WP_024524583.1 ribosome hibernation promoting factor -
  PHA77_RS14160 (PHA77_14160) ptsN 3061000..3061464 (+) 465 WP_005281697.1 PTS IIA-like nitrogen regulatory protein PtsN -
  PHA77_RS14165 (PHA77_14165) rapZ 3061572..3062423 (+) 852 WP_005281696.1 RNase adapter RapZ -
  PHA77_RS14170 (PHA77_14170) npr 3062431..3062703 (+) 273 WP_005281695.1 PTS phosphocarrier protein NPr -
  PHA77_RS14175 (PHA77_14175) rnk 3062880..3063290 (+) 411 WP_035597411.1 nucleoside diphosphate kinase regulator -
  PHA77_RS14180 (PHA77_14180) - 3063321..3063836 (-) 516 WP_116605303.1 cytochrome b/b6 domain-containing protein -
  PHA77_RS14185 (PHA77_14185) cybC 3063842..3064228 (-) 387 WP_005291336.1 cytochrome b562 -
  PHA77_RS14190 (PHA77_14190) deoD 3064442..3065167 (-) 726 WP_005281692.1 purine-nucleoside phosphorylase -

Sequence

Protein


Download         Length: 489 a.a.        Molecular weight: 55075.91 Da        Isoelectric Point: 4.4068

>NTDB_id=779017 PHA77_RS14150 WP_005281701.1 3059105..3060574(+) (rpoN/rpoN1) [Edwardsiella tarda strain SC002]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQEIQQALESNPLLEQLDPNEEAGDDGVVRENTQEDVVTRDDADPLD
SREALEQREMPDELPLDASWDEIYTAGTPSGTGTDYHDDELPVFQGETTQTLQDYLMWQVELTPFSDTDLAIATAIVDAV
DDTGYLSASLEEILESLGDDRVLPEEIEAVLKRIQRFDPVGVAARDLRDCLLVQLSQFADDTPYLNEARSIVDRHLDLLG
NHDFRNLMRSTKLKEEVLKAAMQLIQSLDPRPGLSINTGESDYVIPDVLVHKHQERWVVELNQDSVPRLRINQHYAALGT
QARNDSDSQFIRSNLQEAKWLIKSLESRNDTLLKVSRCIVEQQQAFFEHGPEAMKPMVLADIAQQVEMHESTISRVTTQK
FLHSPRGIFELKYFFSSHVSTEDGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTLLADQGIMVARRTVAKYRESLSIP
PSNQRKQLV

Nucleotide


Download         Length: 1470 bp        

>NTDB_id=779017 PHA77_RS14150 WP_005281701.1 3059105..3060574(+) (rpoN/rpoN1) [Edwardsiella tarda strain SC002]
ATGAAGCAAGGCTTGCAACTCAGACTCAGCCAGCAGTTGGCCATGACGCCCCAACTGCAACAGGCCATTCGCCTGTTGCA
GCTCTCGACGCTGGAGTTACAGCAAGAGATCCAGCAGGCGTTGGAAAGCAATCCTCTGCTGGAACAACTGGATCCCAATG
AGGAAGCCGGCGACGACGGCGTCGTTCGCGAGAATACACAGGAGGATGTCGTCACACGCGATGATGCCGATCCACTGGAT
AGCCGCGAGGCGTTGGAGCAACGTGAGATGCCGGACGAGCTGCCGCTGGATGCCAGCTGGGACGAGATCTATACCGCTGG
CACCCCCTCAGGCACGGGAACGGATTATCATGACGACGAGTTACCCGTGTTTCAGGGAGAGACGACCCAAACGCTGCAGG
ACTATCTGATGTGGCAGGTCGAACTGACCCCCTTCAGTGATACCGATCTGGCCATCGCTACCGCCATCGTCGATGCCGTC
GACGATACCGGCTATCTCAGCGCCTCGTTGGAGGAGATCCTCGAGAGTCTAGGGGACGATCGCGTCCTACCGGAAGAGAT
CGAGGCCGTCCTCAAGCGCATCCAACGTTTTGATCCGGTCGGTGTCGCCGCGCGTGATCTGCGCGACTGCCTACTGGTCC
AGCTCTCGCAATTCGCCGACGACACGCCCTATCTCAACGAGGCTCGTAGCATCGTCGATCGCCACCTCGATCTGTTGGGT
AATCACGATTTCCGAAATCTGATGCGTTCGACTAAGCTAAAAGAAGAGGTGCTCAAGGCGGCGATGCAACTGATCCAGAG
CCTCGACCCGCGGCCAGGACTCTCCATCAATACCGGCGAGTCTGACTATGTCATCCCCGACGTGCTGGTGCATAAACATC
AGGAACGCTGGGTGGTTGAGCTCAATCAGGACAGCGTCCCGCGCCTGCGTATCAATCAACATTACGCCGCGCTGGGCACT
CAGGCGCGTAACGATAGCGACAGCCAGTTCATTCGTAGCAATCTGCAAGAAGCGAAGTGGCTGATCAAGAGCCTAGAGAG
CCGTAACGATACCCTGCTGAAGGTATCGCGTTGCATCGTAGAGCAGCAGCAAGCGTTCTTCGAGCACGGGCCGGAGGCGA
TGAAGCCGATGGTGCTGGCAGATATCGCTCAGCAAGTCGAAATGCACGAGTCGACGATCTCACGCGTGACTACGCAGAAG
TTTCTGCACAGCCCGCGCGGAATCTTTGAACTGAAATATTTCTTCTCCAGCCATGTCAGCACCGAGGACGGCGGCGAGGC
CTCCTCCACTGCCATCCGGGCGCTGGTGAAGAAACTGATCGCGGCAGAAAATCCGGCGAAGCCGCTGAGTGATAGCAAAC
TTACCACTCTATTGGCAGATCAGGGTATCATGGTCGCCCGGCGTACTGTCGCAAAATACCGAGAGTCTTTGTCCATTCCA
CCGTCCAATCAGCGTAAACAGCTGGTGTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(329-487)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(128-315)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D4F169

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.367

100

0.481