Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   PE049_RS01505 Genome accession   NZ_CP116617
Coordinates   316717..318090 (+) Length   457 a.a.
NCBI ID   WP_088460507.1    Uniprot ID   -
Organism   Limosilactobacillus fermentum strain PC76     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 311717..323090
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PE049_RS01475 (PE049_01475) - 311729..312604 (+) 876 WP_057194464.1 alpha/beta hydrolase -
  PE049_RS01480 (PE049_01480) - 312910..314247 (-) 1338 WP_316420599.1 C1 family peptidase -
  PE049_RS01485 (PE049_01485) - 314311..314925 (-) 615 WP_316420898.1 sugar O-acetyltransferase -
  PE049_RS01490 (PE049_01490) rpiA 314956..315642 (-) 687 WP_021815661.1 ribose-5-phosphate isomerase RpiA -
  PE049_RS01495 (PE049_01495) - 315654..315965 (-) 312 WP_316420600.1 GNAT family N-acetyltransferase -
  PE049_RS01500 (PE049_01500) - 316165..316704 (+) 540 WP_004563338.1 dUTP diphosphatase -
  PE049_RS01505 (PE049_01505) radA 316717..318090 (+) 1374 WP_088460507.1 DNA repair protein RadA Machinery gene
  PE049_RS01510 (PE049_01510) - 318123..319256 (+) 1134 WP_316420601.1 PIN/TRAM domain-containing protein -
  PE049_RS01515 (PE049_01515) gltX 319327..320823 (+) 1497 WP_012390831.1 glutamate--tRNA ligase -
  PE049_RS01520 (PE049_01520) - 320891..321460 (+) 570 WP_316420602.1 TIGR01440 family protein -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49353.74 Da        Isoelectric Point: 8.7358

>NTDB_id=778892 PE049_RS01505 WP_088460507.1 316717..318090(+) (radA) [Limosilactobacillus fermentum strain PC76]
MAKAKTHFVCQNCGYNSPRYLGRCPNCGQWSTLVEEVEQASTPAAKNATATLTGIVARPQRIQEIDTKKTPRVKTRLNEL
NRVLGGGIVPGSLVLIGGDPGIGKSTLLLQVSGQLSVEHHKVLYVSGEESASQIKLRAQRLDVSGEDFYIYPETNMEAIR
ATIDSIQPEFVIIDSVQTMQAADVTSAIGSVSQIRAVTAQLMQIAKGQNITVFVVGHVTKGGALAGPKILEHMVDTVLYF
EGDLHHTYRILRSVKNRFGSTNELGIFEMNTRGLTEVANPSEIFLEERLKDATGSAVVVSLEGTRPILVEIQALITPTVF
GNAQRTATGLNRNRVSLIMAVLEKRANLLLQNQDAYLKAAGGVKLDEPAIDLAIAVAIASSYRDKGTRPTDAFVGEVGLT
GEIRRVSRIEQRVAEAAKLGFKRILVPKNNLAGWNPPQDIEVVGVTTLGEALKIALG

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=778892 PE049_RS01505 WP_088460507.1 316717..318090(+) (radA) [Limosilactobacillus fermentum strain PC76]
ATGGCAAAAGCCAAAACACACTTTGTCTGCCAAAACTGCGGTTACAATTCACCGCGCTATTTAGGGCGGTGTCCCAATTG
TGGCCAGTGGAGTACCTTAGTTGAAGAAGTCGAGCAGGCAAGTACCCCGGCGGCTAAAAACGCTACGGCAACCTTAACCG
GGATTGTGGCCCGGCCCCAGCGAATCCAAGAAATTGACACCAAAAAAACGCCCCGGGTTAAGACCCGCTTAAATGAGTTA
AACCGGGTGCTAGGTGGCGGAATTGTACCCGGCTCGTTGGTCTTAATTGGTGGGGATCCCGGGATCGGGAAGTCCACCCT
GCTCTTACAAGTGTCGGGGCAACTGAGCGTGGAACACCACAAGGTTCTTTACGTTTCCGGGGAAGAATCGGCCTCCCAAA
TTAAATTGCGGGCCCAACGCTTAGACGTTTCCGGCGAGGACTTTTATATCTACCCGGAAACCAACATGGAAGCCATCCGG
GCGACGATTGATTCCATTCAGCCCGAGTTTGTAATCATTGACTCGGTTCAGACAATGCAGGCGGCCGACGTGACCTCGGC
GATTGGGTCGGTCTCCCAAATCCGGGCGGTCACCGCCCAGTTGATGCAGATTGCCAAGGGGCAAAACATCACGGTCTTCG
TGGTTGGTCACGTCACCAAGGGTGGGGCCTTAGCCGGTCCCAAGATCTTGGAACACATGGTTGACACGGTGCTGTACTTT
GAAGGGGACCTCCACCACACCTACCGGATTTTACGGTCGGTCAAAAACCGGTTTGGGTCCACCAATGAGTTAGGGATCTT
CGAAATGAATACCCGCGGTTTGACCGAGGTCGCAAACCCCTCGGAAATCTTTTTGGAAGAGCGGCTTAAGGATGCCACCG
GGTCGGCCGTGGTCGTATCCCTGGAGGGAACCCGACCGATCCTGGTTGAAATCCAGGCCTTGATCACGCCAACCGTCTTT
GGAAATGCCCAAAGAACGGCGACGGGTTTAAACCGCAACCGGGTTTCCTTGATCATGGCGGTCCTAGAAAAACGGGCGAA
CCTGCTCTTACAAAACCAGGATGCCTACTTGAAGGCGGCCGGGGGCGTTAAGTTAGACGAGCCGGCCATCGACCTGGCGA
TCGCGGTGGCAATTGCTTCTTCTTACCGGGATAAGGGGACCCGGCCGACCGACGCCTTCGTCGGTGAAGTTGGCTTAACC
GGGGAGATCCGTCGGGTGAGCCGAATTGAACAGCGGGTGGCCGAAGCCGCTAAACTAGGCTTTAAGCGGATTTTAGTGCC
CAAAAACAATTTAGCGGGTTGGAACCCGCCGCAAGATATCGAAGTCGTGGGGGTAACGACCCTTGGCGAAGCACTTAAAA
TTGCCCTTGGATAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(64-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

68.212

99.125

0.676
  radA Streptococcus mitis NCTC 12261

67.991

99.125

0.674
  radA Streptococcus pneumoniae Rx1

67.991

99.125

0.674
  radA Streptococcus pneumoniae D39

67.991

99.125

0.674
  radA Streptococcus pneumoniae R6

67.991

99.125

0.674
  radA Streptococcus pneumoniae TIGR4

67.991

99.125

0.674
  radA Bacillus subtilis subsp. subtilis str. 168

63.676

100

0.637