Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   PML78_RS07085 Genome accession   NZ_CP116583
Coordinates   1515481..1516851 (-) Length   456 a.a.
NCBI ID   WP_016173158.1    Uniprot ID   S1NBV1
Organism   Enterococcus dispar strain K205-4b     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1510481..1521851
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PML78_RS07065 (PML78_07065) epsC 1510847..1511377 (-) 531 WP_016173154.1 serine O-acetyltransferase EpsC -
  PML78_RS07070 (PML78_07070) gltX 1512154..1513611 (-) 1458 WP_195516858.1 glutamate--tRNA ligase -
  PML78_RS07075 (PML78_07075) ispF 1513608..1514108 (-) 501 WP_195516856.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  PML78_RS07080 (PML78_07080) - 1514101..1515342 (-) 1242 WP_262929524.1 PIN/TRAM domain-containing protein -
  PML78_RS07085 (PML78_07085) radA 1515481..1516851 (-) 1371 WP_016173158.1 DNA repair protein RadA Machinery gene
  PML78_RS07090 (PML78_07090) - 1516915..1517424 (-) 510 WP_271856636.1 dUTP diphosphatase -
  PML78_RS07095 (PML78_07095) proB 1518028..1518834 (+) 807 WP_016173160.1 glutamate 5-kinase -
  PML78_RS07100 (PML78_07100) - 1519065..1520312 (+) 1248 WP_016173161.1 glutamate-5-semialdehyde dehydrogenase -
  PML78_RS07105 (PML78_07105) - 1520494..1520787 (-) 294 WP_271856639.1 hypothetical protein -
  PML78_RS07110 (PML78_07110) - 1521051..1521356 (+) 306 WP_016173163.1 hypothetical protein -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49553.33 Da        Isoelectric Point: 8.7868

>NTDB_id=778623 PML78_RS07085 WP_016173158.1 1515481..1516851(-) (radA) [Enterococcus dispar strain K205-4b]
MAKKPKVQFECQHCGYISPKYLGKCPNCGSWNSMVEVVIQDTTDRRVRTSLTGEKTKPTKIADVIPKKEPRVKTKLAELN
RVLGGGVVPGSMVLIGGDPGIGKSTLLLQVSQQLAESGGKVLYVSGEESAEQIKMRAERLGSIKTEFYLYAETDMHEISR
AIENLAPDYVIIDSIQTMTQPDVTSVAGSVSQVRETTAELLKIAKSNGIAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFE
GEKHHTFRILRAVKNRFGSTNEIGIFEMHEDGLHEVANPSQVFLEERLAGATGSAIVVAMEGSRPILVEIQALVTPTMFG
NAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAVAVAIASSYKEKGTSPTECFIGEIGLTG
EIRRVSNIEQRVKEVQKLGFTKVYLPKNNLSGWQAPKGIQVVGVATLAETLKKVFG

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=778623 PML78_RS07085 WP_016173158.1 1515481..1516851(-) (radA) [Enterococcus dispar strain K205-4b]
GTGGCAAAAAAGCCAAAAGTCCAATTTGAATGTCAACATTGTGGCTATATCTCACCAAAATATCTAGGGAAATGTCCCAA
CTGTGGCAGTTGGAATTCCATGGTGGAAGTGGTGATTCAAGATACAACAGACCGCCGCGTCCGCACGAGTTTAACTGGTG
AAAAAACCAAGCCAACAAAAATTGCCGATGTCATTCCTAAAAAAGAACCACGCGTTAAAACCAAACTCGCGGAATTAAAT
CGTGTTTTAGGTGGCGGGGTAGTCCCTGGCTCAATGGTGTTAATCGGAGGAGATCCTGGGATTGGGAAATCAACTTTGCT
TTTACAAGTTTCCCAGCAACTGGCAGAATCAGGCGGCAAAGTTTTATATGTTTCCGGTGAAGAAAGCGCTGAACAAATTA
AAATGCGGGCCGAGCGCTTAGGCTCCATTAAAACCGAGTTTTATTTGTATGCCGAAACGGACATGCATGAAATCTCGCGC
GCGATTGAAAATTTGGCGCCGGACTATGTCATTATCGACTCCATTCAAACTATGACCCAGCCAGATGTTACCAGTGTGGC
CGGCAGCGTTTCACAGGTGAGAGAAACCACCGCTGAATTATTAAAGATTGCCAAATCAAATGGTATTGCAATTTTTATTG
TCGGTCATGTGACCAAAGAAGGTTCCATTGCCGGACCGCGGATGTTAGAGCATATGGTGGATACTGTTTTGTATTTTGAA
GGGGAAAAACACCATACTTTCCGAATTTTACGGGCAGTAAAAAACCGCTTTGGCTCCACTAATGAAATTGGCATTTTTGA
AATGCATGAAGATGGCCTCCATGAAGTGGCTAATCCCTCTCAAGTTTTCTTAGAAGAACGTTTAGCAGGGGCCACGGGTT
CGGCCATTGTCGTTGCCATGGAAGGCAGTCGGCCGATTTTAGTCGAAATTCAGGCCCTTGTCACGCCAACCATGTTTGGT
AACGCCAAACGCACGACCACAGGGCTTGATTTTAATCGGGTTTCGCTGATTATGGCTGTTTTGGAAAAACGGGCGGGGCT
GCTTTTGCAAAATCAAGATGCATATTTAAAAGCTGCTGGTGGCGTAAAATTAAATGAGCCGGCGATTGATTTAGCCGTAG
CGGTAGCCATTGCGTCTAGTTACAAAGAAAAAGGAACCAGTCCCACCGAGTGTTTTATCGGTGAAATCGGCTTAACTGGT
GAAATTCGCCGCGTCTCAAATATTGAACAACGAGTTAAAGAAGTACAAAAATTGGGATTCACAAAGGTATACTTGCCTAA
AAATAATTTGAGTGGCTGGCAGGCACCAAAAGGCATCCAAGTTGTCGGTGTGGCGACACTGGCCGAAACATTAAAAAAAG
TTTTTGGCTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(71-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S1NBV1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

74.725

99.781

0.746
  radA Streptococcus pneumoniae Rx1

74.725

99.781

0.746
  radA Streptococcus pneumoniae D39

74.725

99.781

0.746
  radA Streptococcus pneumoniae R6

74.725

99.781

0.746
  radA Streptococcus pneumoniae TIGR4

74.725

99.781

0.746
  radA Streptococcus mitis SK321

74.505

99.781

0.743
  radA Bacillus subtilis subsp. subtilis str. 168

66.154

99.781

0.66