Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   PML98_RS13390 Genome accession   NZ_CP116581
Coordinates   2888179..2889549 (-) Length   456 a.a.
NCBI ID   WP_005876903.1    Uniprot ID   A0A377KI18
Organism   Enterococcus durans strain R59-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2883179..2894549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PML98_RS13375 (PML98_13375) epsC 2884442..2884978 (-) 537 WP_193809306.1 serine O-acetyltransferase EpsC -
  PML98_RS13380 (PML98_13380) gltX 2885300..2886760 (-) 1461 WP_005876899.1 glutamate--tRNA ligase -
  PML98_RS13385 (PML98_13385) - 2886997..2888142 (-) 1146 WP_005876901.1 PIN/TRAM domain-containing protein -
  PML98_RS13390 (PML98_13390) radA 2888179..2889549 (-) 1371 WP_005876903.1 DNA repair protein RadA Machinery gene
  PML98_RS13395 (PML98_13395) - 2889597..2890130 (-) 534 WP_005876905.1 SPbeta phage deoxyuridine 5'-triphosphate nucleotidohydrolase -
  PML98_RS13400 (PML98_13400) proB 2890337..2891143 (+) 807 WP_005876906.1 glutamate 5-kinase -
  PML98_RS13405 (PML98_13405) - 2891140..2892384 (+) 1245 WP_193809305.1 glutamate-5-semialdehyde dehydrogenase -
  PML98_RS13410 (PML98_13410) - 2892482..2892826 (+) 345 WP_193809304.1 hypothetical protein -
  PML98_RS13415 (PML98_13415) - 2893376..2893687 (-) 312 WP_081135289.1 hypothetical protein -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49897.55 Da        Isoelectric Point: 7.3892

>NTDB_id=778602 PML98_RS13390 WP_005876903.1 2888179..2889549(-) (radA) [Enterococcus durans strain R59-1]
MAKKAKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTSDRRTRTSLTGEKAKPTKIAEVIPKKESRVKTKLAELN
RVLGGGVVPGSMILIGGDPGIGKSTLLLQVSQQLADIGGKVLYVSGEESAEQIKLRAERLGSIDTEFYLYAETDMNEISR
AIEQISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFE
GEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEVANPSQIFLEERLEGATGSAIVVAMEGTRPILVEIQALVTPTMFG
NAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAISIASSYKEKGTQPTECFIGEIGLTG
EIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGGWQPPQGIEVVGVATLAETLKRVFR

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=778602 PML98_RS13390 WP_005876903.1 2888179..2889549(-) (radA) [Enterococcus durans strain R59-1]
ATGGCAAAAAAAGCAAAAGTGCAATTTGTCTGTCAGAACTGTGGGTATGTTTCGCCTAAATTTTTAGGGCGATGCCCGAA
TTGTGGAAAATGGAATACGATGGTGGAAGAAATTGAGCAGGATACTAGCGACCGCAGAACTAGGACGAGCTTAACTGGTG
AAAAAGCGAAGCCAACTAAAATTGCGGAAGTCATCCCCAAAAAAGAATCACGGGTAAAAACGAAACTTGCTGAATTGAAT
CGAGTATTAGGCGGCGGTGTTGTTCCAGGATCAATGATCTTGATCGGTGGAGATCCAGGTATCGGGAAATCGACGTTGTT
ATTACAAGTTTCACAACAACTTGCAGACATTGGTGGTAAAGTATTGTATGTATCTGGTGAAGAAAGTGCTGAGCAAATCA
AGCTACGTGCGGAACGCCTAGGATCGATCGACACGGAATTTTATTTATACGCAGAAACAGATATGAATGAGATCAGCCGA
GCAATTGAACAAATCTCTCCTGATTATGTCATTATTGATTCCATCCAAACAATGACACAACCGGATATCACGAGTGTAGC
AGGTAGCGTGAGTCAAGTGCGGGAAACGACTGCAGAATTATTAAAAATTGCCAAAACGAACGGTATCGCTATTTTTATCG
TTGGACATGTCACAAAAGAAGGGTCGATTGCAGGTCCAAGAATGCTCGAACACATGGTAGATACGGTTCTTTATTTCGAA
GGAGAACAACACCATAGTTTCCGTATTTTAAGAGCGGTGAAGAATCGATTTGGTTCGACAAATGAAATTGGTATTTTTGA
GATGCACGAGCATGGATTGGAGGAAGTGGCGAATCCTTCGCAGATTTTCTTAGAAGAGCGCTTAGAAGGCGCAACCGGTT
CAGCCATCGTGGTGGCGATGGAAGGCACTCGTCCTATTTTAGTAGAGATCCAAGCACTTGTGACACCCACGATGTTCGGA
AACGCAAAACGAACAACGACTGGCTTAGATTTTAACCGAGTCTCACTGATCATGGCTGTCCTAGAAAAAAGAGCAGGCTT
ATTGTTGCAGAATCAAGATGCTTACTTAAAAGCGGCTGGTGGGGTAAAATTAAATGAGCCGGCCATTGATTTAGCTATTG
CGATCAGTATTGCTTCGAGTTACAAAGAAAAAGGAACACAGCCAACAGAATGCTTTATTGGCGAAATCGGTTTGACTGGT
GAGATCAGACGGGTAAGTCATATTGAGCAGAGGGTCAAAGAAGTACAGAAATTAGGATTCACAAAAGTGTATCTGCCAAA
AAATAATCTTGGCGGATGGCAACCGCCTCAGGGAATCGAAGTAGTGGGCGTAGCCACATTAGCTGAAACATTAAAACGTG
TGTTTCGCTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

AAA domain (AAA_25)

(66-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A377KI18

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

74.286

99.781

0.741
  radA Streptococcus mitis SK321

74.286

99.781

0.741
  radA Streptococcus pneumoniae Rx1

74.066

99.781

0.739
  radA Streptococcus pneumoniae D39

74.066

99.781

0.739
  radA Streptococcus pneumoniae R6

74.066

99.781

0.739
  radA Streptococcus pneumoniae TIGR4

74.066

99.781

0.739
  radA Bacillus subtilis subsp. subtilis str. 168

67.406

98.904

0.667