Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ICHIJ1_RS00495 Genome accession   NZ_AP022347
Coordinates   93756..95255 (+) Length   499 a.a.
NCBI ID   WP_162048791.1    Uniprot ID   -
Organism   Fluviibacter phosphoraccumulans strain ICHIJ1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 88756..100255
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ICHIJ1_RS00475 (ICHIJ1_00920) - 89155..91191 (-) 2037 WP_242451476.1 UvrD-helicase domain-containing protein -
  ICHIJ1_RS00480 (ICHIJ1_00930) recA 91334..92365 (+) 1032 WP_162048788.1 recombinase RecA Machinery gene
  ICHIJ1_RS00485 (ICHIJ1_00940) - 92388..92948 (+) 561 WP_174237188.1 regulatory protein RecX -
  ICHIJ1_RS00490 (ICHIJ1_00950) lptB 92980..93717 (+) 738 WP_162048790.1 LPS export ABC transporter ATP-binding protein -
  ICHIJ1_RS00495 (ICHIJ1_00960) rpoN/rpoN1 93756..95255 (+) 1500 WP_162048791.1 RNA polymerase factor sigma-54 Machinery gene
  ICHIJ1_RS00500 (ICHIJ1_00970) hpf 95266..95586 (+) 321 WP_162048792.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ICHIJ1_RS00505 (ICHIJ1_00980) - 95615..96088 (+) 474 WP_242451477.1 PTS sugar transporter subunit IIA -
  ICHIJ1_RS00510 (ICHIJ1_00990) hprK 96104..97123 (+) 1020 WP_162048793.1 HPr(Ser) kinase/phosphatase -
  ICHIJ1_RS00515 (ICHIJ1_01000) ttcA 97128..98057 (-) 930 WP_162048794.1 tRNA 2-thiocytidine(32) synthetase TtcA -
  ICHIJ1_RS00520 (ICHIJ1_01010) - 98070..98816 (-) 747 WP_162048795.1 pteridine reductase -
  ICHIJ1_RS00525 (ICHIJ1_01020) - 98927..100159 (+) 1233 WP_162048796.1 class I SAM-dependent methyltransferase -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 54826.55 Da        Isoelectric Point: 4.5879

>NTDB_id=77842 ICHIJ1_RS00495 WP_162048791.1 93756..95255(+) (rpoN/rpoN1) [Fluviibacter phosphoraccumulans strain ICHIJ1]
MKASLQLKGTQHLTMTPQLQQSIRLLQLSSVELQQEVAQALAENPMLERDDAEEGEGNDTDATTTADNNHTHQEFSNETR
AEFNEPRAEPSDNGNEGMTDAAEAFEQSSPMDMDEGPYAYSGSGGEDNDSDYDADGSGGITLREHLLTQVGLQRCSWREQ
ALIRILIEALDERGYLTSGLEEILAALPADEAVTLPEIEAALAQLQELDPLGVGARTLNECLLLQLKELIDPAADLARSL
VTDYLPALAQRDFVLLRRKTGADDNALRDAQALIRSLNPHPGHSFLDDASPYIIPDVLVRKTPKAKADRWMATTNPDALP
RLKINRLYAGLLRQASGGHSGLTGQLQEARWLIKNIQQRFETIERVAQAIVDEQAAFFEKGELAMRPLVLREIADQLGLH
ESTISRVTSQKFMATPRGVFEFKYFFGSHVGTDDGGAASATAIKARIRQLIGGENPKKPLSDQQICDQFSAEGIVVARRT
IAKYREAMGLPTASLRKTL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=77842 ICHIJ1_RS00495 WP_162048791.1 93756..95255(+) (rpoN/rpoN1) [Fluviibacter phosphoraccumulans strain ICHIJ1]
ATGAAGGCCAGCCTGCAACTCAAAGGCACGCAGCACCTGACAATGACCCCGCAATTGCAACAGTCGATCCGACTGCTGCA
ACTGTCCTCGGTCGAACTGCAACAAGAAGTGGCGCAAGCGCTGGCTGAAAACCCCATGCTCGAACGCGATGATGCAGAAG
AGGGCGAGGGCAACGACACCGACGCCACAACAACGGCCGATAACAACCATACGCATCAAGAGTTTTCTAACGAAACGCGC
GCAGAATTTAATGAGCCGCGTGCTGAGCCTAGCGATAACGGCAACGAAGGCATGACAGATGCCGCCGAGGCCTTCGAGCA
GTCCAGCCCCATGGATATGGATGAAGGCCCCTACGCCTACTCGGGATCCGGCGGAGAGGATAACGACAGCGACTACGATG
CAGATGGCAGCGGTGGCATTACCCTGCGTGAACATCTGCTCACCCAGGTCGGTCTTCAACGCTGCAGCTGGCGTGAACAA
GCCCTCATACGCATTCTGATCGAAGCCCTCGACGAACGGGGTTATCTGACCAGCGGACTGGAAGAGATTCTGGCCGCACT
GCCAGCAGATGAGGCAGTTACCCTGCCAGAAATTGAAGCGGCTCTGGCGCAACTACAAGAGCTCGACCCACTCGGCGTCG
GGGCACGCACGCTGAATGAATGTTTATTGCTGCAACTGAAAGAACTGATCGACCCCGCGGCCGATCTGGCCCGTAGCCTG
GTCACCGACTACCTACCTGCACTCGCGCAGCGTGATTTCGTCCTGCTGAGACGCAAAACCGGGGCCGACGACAATGCCTT
GCGCGATGCCCAGGCGCTGATTCGTAGCCTGAATCCGCACCCGGGTCATAGCTTCCTCGACGATGCCAGCCCGTACATCA
TTCCCGATGTTCTGGTGCGCAAAACCCCCAAAGCTAAAGCCGATCGCTGGATGGCCACGACCAATCCGGATGCTTTGCCT
CGACTCAAAATCAATCGGCTCTATGCCGGTTTGCTTCGGCAAGCGAGCGGTGGCCACAGTGGCCTCACGGGGCAGCTGCA
AGAAGCCCGTTGGCTCATCAAGAATATCCAGCAACGTTTCGAGACCATCGAACGGGTGGCGCAGGCCATTGTGGACGAAC
AGGCCGCTTTTTTTGAAAAAGGTGAGCTGGCCATGCGCCCGCTGGTCCTGCGCGAAATTGCCGACCAACTGGGCTTGCAC
GAATCCACCATTTCCCGCGTTACTTCCCAGAAATTCATGGCGACGCCGCGCGGAGTCTTTGAATTTAAATACTTTTTTGG
CAGCCATGTCGGCACGGATGACGGTGGCGCCGCTTCCGCCACTGCCATTAAAGCCCGAATCCGTCAGCTGATCGGGGGAG
AGAATCCGAAAAAACCACTCTCGGACCAACAAATTTGTGATCAATTTAGTGCCGAAGGCATTGTCGTAGCACGCCGTACC
ATAGCAAAATACCGTGAGGCGATGGGTCTTCCCACCGCCAGTTTGCGCAAGACCCTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(140-328)

Sigma-54, DNA binding domain (Sigma54_DBD)

(343-497)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.961

100

0.531

Multiple sequence alignment