Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   PML82_RS10920 Genome accession   NZ_CP116514
Coordinates   2230442..2232250 (-) Length   602 a.a.
NCBI ID   WP_002325324.1    Uniprot ID   A0A9X3XTJ6
Organism   Enterococcus faecium strain K195-6b     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 2225442..2237250
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PML82_RS10895 (PML82_10895) - 2226482..2227381 (-) 900 WP_002301297.1 RluA family pseudouridine synthase -
  PML82_RS10900 (PML82_10900) - 2227383..2228180 (-) 798 WP_002289847.1 NAD kinase -
  PML82_RS10905 (PML82_10905) - 2228158..2228862 (-) 705 WP_002289848.1 GTP pyrophosphokinase -
  PML82_RS10910 (PML82_10910) - 2228993..2229568 (+) 576 WP_002289849.1 CYTH domain-containing protein -
  PML82_RS10915 (PML82_10915) - 2229686..2230363 (+) 678 WP_002289850.1 ClpXP adapter SpxH family protein -
  PML82_RS10920 (PML82_10920) pepF 2230442..2232250 (-) 1809 WP_002325324.1 oligoendopeptidase F Regulator
  PML82_RS10925 (PML82_10925) - 2232426..2233424 (-) 999 WP_002325323.1 competence protein CoiA -
  PML82_RS10930 (PML82_10930) - 2233651..2234304 (-) 654 WP_002299370.1 adaptor protein MecA -
  PML82_RS10935 (PML82_10935) spxA 2234636..2235034 (-) 399 WP_002296384.1 transcriptional regulator SpxA -
  PML82_RS10940 (PML82_10940) - 2235799..2236506 (+) 708 WP_002294563.1 HAD family hydrolase -

Sequence

Protein


Download         Length: 602 a.a.        Molecular weight: 69683.94 Da        Isoelectric Point: 4.6498

>NTDB_id=777780 PML82_RS10920 WP_002325324.1 2230442..2232250(-) (pepF) [Enterococcus faecium strain K195-6b]
MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQSEKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAH
LKNDQDTGNTDYQALYARASSLFSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAAFKGLYSVYEQFRNTFASTLGTH
IKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVDVVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTY
EEAKEKALEALKPMGEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHEMGHSVHSY
FTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHYLDGFKGTVFRQTQFAEFEHFMHTEDEKGVP
LTSEYLSDSYGKLNAKYYGPAVEEDPEIKFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGN
SDYPVEVMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDHL

Nucleotide


Download         Length: 1809 bp        

>NTDB_id=777780 PML82_RS10920 WP_002325324.1 2230442..2232250(-) (pepF) [Enterococcus faecium strain K195-6b]
ATGGAAGTAAAGCAGTTGCCAAAACGAGAAGAATTGCCTGAAAATTTAACTTGGGACTTGACCAAGATCTTTTCAAGCGA
CCAAGAGTTTGATGAGAAATATTTGGAATTATCAGAAGAGTTAAAACAATCTGAAAAACACAAAGGAACACTTGATCAAG
GCGCTTCTCAATTTTTAAATGCGATTGAATTCGTATTGAGGGTTTATCGCCAAACTGAAGTCATTTATGTATATGCGCAC
CTTAAAAACGATCAAGACACTGGAAATACAGATTACCAAGCGCTTTATGCAAGAGCAAGCAGTCTGTTTTCGAAAGTTAG
TGAAGCCGTTTCCTGGTTTGAACCAGAAATATTGCAATTGTCAGATGACCAGATTTGGCAATATTTCAAAGAAGAACCAA
AATTGGAAGTCTATCGCCATTATATCCAGCAAATAGTAGATAATCGAGCCCATGTCTTATCTGCTGAGCAGGAATCTCTT
CTTGCTGGAGCAGGTGAAATCTTTGATGCTTCAAGTGATACATTTGCTGTTTTGAATAATGCAGATCTAGTTTTTCCAAC
GATTGAAGGAGAAAATGGTGAAATAGTCCAATTATCTCATGGCGTGTATGGTCAGTTGCTAGAAAGCACGGATCGAAGGG
TACGCGAAGCAGCATTTAAGGGATTGTACAGTGTTTACGAACAATTTAGAAATACATTTGCTTCTACTTTAGGCACACAT
ATAAAAGGACATAATTTTAAAGCGAAAGTCCGTAATTATAGCTCTGCCAGAGAAGCGTCTTTGAGCAATAATCATATTCC
TGAAAGTGTATACGATACTTTGGTAGACGTGGTAAACAAGCATTTGCCTTTGTTACATCGATACATGGAATTACGGAAAC
GTTTATTAGAAGTGGAAAAACTGCACATGTATGATCTTTATACACCGGTCTTAGGGGAAGCTCCAATTACCTTTACGTAC
GAAGAAGCAAAAGAAAAAGCTTTAGAAGCACTGAAACCAATGGGTGAAGAATACATGGCCATCGTAGAAAAAGCATTCTC
TGAACGTTGGATCGATGTTGTCGAAAATAAAGGGAAACGAAGCGGTGCTTATTCTTCGGGAAGCTATGACACAAATCCAT
ATATTTTATTGAATTGGCATGATACGCTGGATCAGCTATTTACGCTTGTCCACGAAATGGGACATAGTGTTCATAGTTAT
TTCACTCGTTCGAACCAGCCTTATGTGTACGGCGACTACTCCATCTTTTTAGCAGAAATTGCTTCGACAACGAATGAAAA
TATCCTAACGGAGTATTTATTGGAAACAGAAAAAGACCCTCGTGTACGGGCTTATGTACTCAACCATTACTTGGATGGGT
TTAAAGGAACAGTTTTCCGCCAGACACAATTCGCTGAATTCGAGCATTTCATGCATACGGAAGATGAAAAAGGTGTGCCA
TTGACTAGTGAATACCTAAGTGATAGTTATGGTAAATTGAATGCAAAATATTATGGTCCAGCAGTCGAAGAAGACCCAGA
AATTAAATTCGAATGGTCACGGATACCGCATTTTTATTATAATTACTATGTTTTCCAATACTCGACTGGCTTTTCTGCTG
CTTCAGCACTGGCGAAGAAAATACTAAACCAGGAACCAGAGGCACTAGAGAACTACTTGGCTTACCTAAAAGCAGGTAAC
AGCGATTATCCTGTGGAAGTAATGAAAAAAGCAGGAGTTGATATGACACAAGCTGCATATATCGAAGATGCAATGTCGAT
GTTTGAACAACGTCTAAATGAATTAGAAGAATTGATCGATCATTTATAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(116-185)

Peptidase family M3 (Peptidase_M3)

(206-586)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

55.743

98.339

0.548