Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   QU831_RS06470 Genome accession   NZ_CP129240
Coordinates   1342772..1343449 (+) Length   225 a.a.
NCBI ID   WP_001377644.1    Uniprot ID   -
Organism   Escherichia coli strain 1344_LBHALD     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1337772..1348449
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QU831_RS06435 (QU831_06435) gspG 1338252..1338689 (+) 438 WP_001202880.1 type II secretion system major pseudopilin GspG -
  QU831_RS06440 (QU831_06440) gspH 1338697..1339206 (+) 510 WP_001076048.1 type II secretion system minor pseudopilin GspH -
  QU831_RS06445 (QU831_06445) gspI 1339203..1339580 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  QU831_RS06450 (QU831_06450) gspJ 1339573..1340160 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  QU831_RS06455 (QU831_06455) gspK 1340153..1341136 (+) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  QU831_RS06460 (QU831_06460) gspL 1341151..1342314 (+) 1164 WP_001115230.1 type II secretion system protein GspL -
  QU831_RS06465 (QU831_06465) gspM 1342311..1342772 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  QU831_RS06470 (QU831_06470) pilD 1342772..1343449 (+) 678 WP_001377644.1 prepilin peptidase GspO Machinery gene
  QU831_RS06475 (QU831_06475) bfr 1343478..1343954 (-) 477 WP_000675504.1 bacterioferritin -
  QU831_RS06480 (QU831_06480) bfd 1344026..1344220 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  QU831_RS06485 (QU831_06485) chiA 1344389..1347082 (-) 2694 WP_001377643.1 bifunctional chitinase/lysozyme -
  QU831_RS06490 (QU831_06490) tuf 1347374..1347523 (-) 150 Protein_1273 elongation factor Tu -
  QU831_RS06495 (QU831_06495) - 1347624..1348046 (+) 423 Protein_1274 hypothetical protein -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24838.75 Da        Isoelectric Point: 7.8986

>NTDB_id=777508 QU831_RS06470 WP_001377644.1 1342772..1343449(+) (pilD) [Escherichia coli strain 1344_LBHALD]
MTMLLPLFILVGFIAGCFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=777508 QU831_RS06470 WP_001377644.1 1342772..1343449(+) (pilD) [Escherichia coli strain 1344_LBHALD]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTGTTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.382
  pilD Vibrio cholerae strain A1552

35.745

100

0.373