Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   PH197_RS00630 Genome accession   NZ_CP116456
Coordinates   137101..138468 (+) Length   455 a.a.
NCBI ID   WP_077281988.1    Uniprot ID   A0A5B8TEG9
Organism   Leuconostoc lactis strain DMLL10     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 132101..143468
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PH197_RS00595 (PH197_00595) - 132526..133419 (+) 894 WP_305016212.1 sulfite exporter TauE/SafE family protein -
  PH197_RS00610 (PH197_00610) - 133712..135136 (-) 1425 WP_305016213.1 aspartate ammonia-lyase -
  PH197_RS00615 (PH197_00615) rpiA 135299..135988 (-) 690 WP_305016214.1 ribose-5-phosphate isomerase RpiA -
  PH197_RS00620 (PH197_00620) - 135985..136266 (-) 282 WP_010002509.1 GNAT family N-acetyltransferase -
  PH197_RS00625 (PH197_00625) - 136492..137031 (+) 540 WP_305016215.1 deoxyuridine 5'-triphosphate nucleotidohydrolase -
  PH197_RS00630 (PH197_00630) radA 137101..138468 (+) 1368 WP_077281988.1 DNA repair protein RadA Machinery gene
  PH197_RS00635 (PH197_00635) - 138479..138802 (+) 324 WP_305016216.1 hypothetical protein -
  PH197_RS00640 (PH197_00640) gltX 138978..140474 (+) 1497 WP_305016217.1 glutamate--tRNA ligase -
  PH197_RS00645 (PH197_00645) - 140610..141515 (+) 906 WP_305016218.1 NADP-dependent oxidoreductase -
  PH197_RS00650 (PH197_00650) adhP 141678..142733 (+) 1056 WP_305016219.1 alcohol dehydrogenase AdhP -
  PH197_RS00655 (PH197_00655) - 142894..143433 (+) 540 WP_305016220.1 hypothetical protein -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 49227.40 Da        Isoelectric Point: 5.2659

>NTDB_id=777392 PH197_RS00630 WP_077281988.1 137101..138468(+) (radA) [Leuconostoc lactis strain DMLL10]
MAKVKTQFICSNCGFTSARYLGRCSNCGEWGTFVEEKIMPETNDRKSRVSLDGRTAKVEKINEVTSEETPRVATNLKELN
RVLGGGVVPGSMVLIGGDPGIGKSTLLLQVSGQLAHEGRVLYVTGEESATQVKLRADRLGVGNDEFYLYPETDMTAIKKQ
IESLQPDFVVIDSVQTMQEPDVTSAIGSVSQIREVTADLLQIAKTNNISIFIVGHVTKDGAIAGPKILEHMVDTVLYFEG
DSNYKYRILRAVKNRFGATNELGIFEMRDGGLIEVANPSEIFLEERLAGATGSAIVVALEGSRPILVELQALVTPTVFGN
AQRTASGLDRNRVSLIMAVLEKRANLLLQNQDAYLKAAGGVKLDEPAIDLAIAVALASSYHDKESRPSDVFVGEIGLTGE
IRSVADIESRLKEARKLGFKRAIVPKNNLNGVTLPEGIQVIGVATLREALQLALD

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=777392 PH197_RS00630 WP_077281988.1 137101..138468(+) (radA) [Leuconostoc lactis strain DMLL10]
ATCGCAAAAGTTAAAACACAATTTATTTGCTCAAACTGTGGTTTCACGTCAGCGCGCTATCTGGGTCGCTGTTCAAACTG
TGGTGAATGGGGCACATTTGTCGAAGAAAAAATTATGCCAGAGACCAACGACCGGAAAAGTCGGGTGAGCCTAGACGGTC
GTACGGCTAAGGTTGAAAAGATTAATGAGGTGACATCAGAGGAAACACCGCGTGTTGCCACGAACCTTAAAGAACTGAAT
CGCGTGTTAGGTGGTGGCGTTGTTCCAGGTTCAATGGTGCTGATTGGTGGGGATCCAGGCATTGGGAAATCAACTCTCTT
GCTCCAAGTCTCTGGGCAATTGGCCCATGAAGGTCGTGTGTTATACGTGACTGGGGAAGAATCTGCCACCCAAGTGAAGT
TACGGGCAGACCGTTTGGGTGTTGGAAATGATGAATTTTATCTTTATCCAGAAACAGATATGACGGCGATTAAAAAGCAA
ATTGAATCGCTGCAACCAGATTTTGTCGTGATTGACTCAGTACAAACGATGCAGGAACCAGACGTGACGTCCGCCATTGG
CTCCGTGTCACAAATCCGCGAAGTCACGGCAGATTTATTGCAAATTGCTAAAACCAATAATATTTCTATTTTTATCGTTG
GTCACGTGACCAAAGATGGGGCGATTGCTGGACCAAAGATTTTGGAGCATATGGTCGATACGGTGCTCTATTTTGAAGGC
GATAGTAACTACAAGTACCGCATTTTACGCGCGGTGAAAAACCGTTTTGGCGCTACCAATGAATTGGGTATTTTTGAAAT
GCGCGACGGCGGTTTGATTGAAGTCGCCAACCCATCAGAAATCTTTTTGGAGGAACGATTAGCTGGCGCAACTGGTTCGG
CCATTGTGGTCGCCTTAGAAGGCTCACGGCCGATTTTAGTGGAGTTGCAAGCTTTGGTGACGCCAACCGTCTTTGGGAAT
GCGCAACGGACGGCATCCGGATTAGATCGTAACCGCGTGTCGCTAATTATGGCCGTTTTGGAAAAGCGCGCCAATCTCTT
GTTACAAAATCAAGATGCTTATCTGAAAGCTGCCGGTGGGGTCAAGCTGGATGAACCAGCAATTGATTTGGCGATTGCCG
TGGCGCTGGCCAGTTCTTATCACGATAAAGAATCGCGGCCAAGCGACGTCTTTGTCGGTGAAATCGGGTTAACTGGGGAA
ATCCGGAGCGTCGCTGATATTGAAAGTCGGTTGAAGGAAGCGCGCAAGCTCGGCTTTAAACGGGCGATTGTGCCAAAGAA
CAACTTGAATGGTGTTACCTTACCCGAAGGGATTCAAGTAATTGGCGTCGCCACATTGCGGGAAGCGTTGCAATTAGCCT
TAGATTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(70-220)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(362-428)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5B8TEG9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

66.962

99.121

0.664
  radA Streptococcus pneumoniae D39

66.962

99.121

0.664
  radA Streptococcus pneumoniae R6

66.962

99.121

0.664
  radA Streptococcus pneumoniae TIGR4

66.962

99.121

0.664
  radA Streptococcus mitis NCTC 12261

66.741

99.121

0.662
  radA Streptococcus mitis SK321

66.741

99.121

0.662
  radA Bacillus subtilis subsp. subtilis str. 168

62.281

100

0.624