Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PL318_RS15430 Genome accession   NZ_CP116403
Coordinates   3441451..3442770 (+) Length   439 a.a.
NCBI ID   WP_004526057.1    Uniprot ID   A0AAX0UF52
Organism   Burkholderia pseudomallei strain MSHR5087     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3436451..3447770
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PL318_RS15400 (PL318_15400) - 3436962..3437576 (+) 615 WP_004200150.1 50S ribosomal protein L25/general stress protein Ctc -
  PL318_RS15405 (PL318_15405) pth 3437690..3438295 (+) 606 WP_004202941.1 aminoacyl-tRNA hydrolase -
  PL318_RS15410 (PL318_15410) hisC 3438384..3439454 (+) 1071 WP_038741564.1 histidinol-phosphate transaminase -
  PL318_RS15415 (PL318_15415) - 3439633..3439899 (-) 267 WP_004195247.1 YfhL family 4Fe-4S dicluster ferredoxin -
  PL318_RS15420 (PL318_15420) coaD 3439962..3440462 (-) 501 WP_004195249.1 pantetheine-phosphate adenylyltransferase -
  PL318_RS15425 (PL318_15425) rsmD 3440573..3441199 (-) 627 WP_004522861.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  PL318_RS15430 (PL318_15430) pilA 3441451..3442770 (+) 1320 WP_004526057.1 signal recognition particle-docking protein FtsY Machinery gene
  PL318_RS15435 (PL318_15435) maiA 3442927..3443571 (-) 645 WP_004541039.1 maleylacetoacetate isomerase -
  PL318_RS15440 (PL318_15440) nirB 3443956..3446400 (+) 2445 WP_045719281.1 nitrite reductase large subunit NirB -
  PL318_RS15445 (PL318_15445) - 3446476..3446871 (+) 396 WP_045719280.1 nitrite reductase (NAD(P)H) small subunit -

Sequence

Protein


Download         Length: 439 a.a.        Molecular weight: 46370.66 Da        Isoelectric Point: 6.0726

>NTDB_id=777186 PL318_RS15430 WP_004526057.1 3441451..3442770(+) (pilA) [Burkholderia pseudomallei strain MSHR5087]
MFSFFKRFKKAPDAAPAEPQQAPDAPRDASRAPASPDAARVDAPAAPQPRDERSENHDRPEHDETPTAEHSRSGAQPAAR
ATPPASAAASEAPVGQPRAAAARASEPAPSVVMTVTPSSNGRHGVVETVEIVPPPAPEPAAKKSWIARLKSGLAKTSSSI
TNVFVNTKIDDALYEELETALLMSDAGIDATEHLLDALREKVRAGRLTDPQQVKDALRGLLVELLAPLEKSLVLGRAQPL
VMMIAGVNGAGKTTSIGKLAKHLQSFDQSVLLAAGDTFRAAAREQLAIWGERNNVTVVQQESGDPAAVIFDAVSAARARN
IDVMMADTAGRLPTQLHLMEELRKVKRVIGKAHDGAPHEVLLVIDANTGQNALAQVKAFDDALGLTGLIVTKLDGTAKGG
ILAAIARQRPIPVYFIGIGEKVEDLQPFSAEEFADALLG

Nucleotide


Download         Length: 1320 bp        

>NTDB_id=777186 PL318_RS15430 WP_004526057.1 3441451..3442770(+) (pilA) [Burkholderia pseudomallei strain MSHR5087]
ATGTTCAGTTTCTTCAAACGATTCAAGAAAGCGCCCGATGCCGCCCCGGCCGAGCCGCAGCAAGCGCCCGACGCGCCGCG
GGACGCTTCCCGCGCACCGGCATCGCCCGACGCCGCGCGAGTCGACGCGCCGGCCGCGCCGCAGCCGCGCGACGAGCGCA
GCGAAAACCACGATCGCCCCGAGCACGACGAAACGCCGACCGCCGAGCATTCCCGTTCCGGCGCGCAGCCCGCCGCGCGC
GCGACGCCGCCCGCGTCCGCCGCCGCGTCGGAAGCGCCGGTCGGGCAGCCGCGCGCCGCCGCCGCGCGCGCAAGCGAGCC
CGCGCCGTCGGTCGTGATGACGGTCACGCCGTCGTCGAACGGCCGCCACGGCGTCGTCGAGACGGTCGAGATCGTGCCGC
CGCCCGCGCCCGAGCCGGCCGCGAAGAAATCGTGGATCGCGCGGCTGAAGTCCGGCCTCGCGAAGACGAGCTCGAGCATC
ACGAACGTATTCGTCAACACGAAAATCGACGACGCGCTCTACGAGGAGCTCGAGACGGCGCTGCTGATGTCCGACGCCGG
CATCGACGCGACCGAGCATCTGCTCGACGCGCTGCGCGAAAAGGTGCGCGCGGGCCGCCTCACCGATCCGCAGCAGGTGA
AGGACGCGCTGCGCGGGCTGCTCGTCGAGCTGCTGGCGCCGCTCGAGAAGTCGCTCGTGCTCGGCCGCGCGCAGCCGCTC
GTGATGATGATCGCGGGCGTGAACGGCGCCGGCAAGACGACGAGCATCGGCAAGCTCGCGAAGCATCTGCAGAGCTTCGA
TCAATCGGTGCTGCTCGCCGCGGGCGACACGTTTCGCGCCGCCGCGCGCGAGCAGCTCGCGATCTGGGGCGAACGCAACA
ACGTGACCGTCGTCCAGCAGGAAAGCGGCGACCCCGCCGCGGTGATCTTCGATGCGGTCAGCGCCGCGCGCGCGCGCAAC
ATCGACGTGATGATGGCCGACACCGCAGGCCGCCTGCCGACGCAGCTGCATCTGATGGAAGAGCTCCGGAAGGTGAAGCG
CGTGATCGGCAAGGCGCACGACGGCGCGCCGCACGAGGTGCTGCTCGTGATCGACGCGAACACCGGCCAGAACGCGCTCG
CGCAGGTAAAGGCGTTCGACGACGCGCTCGGCCTCACGGGCCTCATCGTCACGAAGCTCGACGGCACCGCGAAGGGCGGC
ATCCTCGCCGCGATCGCGCGCCAGCGGCCGATTCCGGTGTACTTCATCGGCATCGGCGAGAAGGTCGAGGATCTGCAGCC
GTTCAGCGCGGAGGAATTCGCGGACGCGCTGCTCGGCTAG

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(158-218)

SRP54-type protein, GTPase domain (SRP54)

(240-439)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

50.113

100

0.506