Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   QWI19_RS12805 Genome accession   NZ_CP129123
Coordinates   2479710..2480120 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain 6D1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2474710..2485120
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QWI19_RS12775 - 2474797..2474937 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  QWI19_RS12780 yqeF 2475243..2475974 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  QWI19_RS12785 cwlH 2476226..2476978 (-) 753 WP_015251672.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  QWI19_RS12790 yqeD 2477165..2477791 (+) 627 WP_015251671.1 TVP38/TMEM64 family protein -
  QWI19_RS12795 gnd 2477810..2478703 (-) 894 WP_015251670.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  QWI19_RS12800 yqeB 2478955..2479677 (+) 723 WP_010886572.1 hypothetical protein -
  QWI19_RS12805 nucA/comI 2479710..2480120 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  QWI19_RS12810 - 2480316..2480666 (+) 351 Protein_2476 sigma-70 family RNA polymerase sigma factor -
  QWI19_RS12815 - 2480806..2481276 (+) 471 WP_015251668.1 MarR family transcriptional regulator -
  QWI19_RS12820 - 2481426..2482838 (+) 1413 WP_015251667.1 MDR family MFS transporter -
  QWI19_RS12825 spoIVCA 2483025..2484485 (-) 1461 WP_235701260.1 site-specific DNA recombinase SpoIVCA -
  QWI19_RS12830 - 2484443..2484621 (-) 179 Protein_2480 hypothetical protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=777039 QWI19_RS12805 WP_009967785.1 2479710..2480120(-) (nucA/comI) [Bacillus subtilis strain 6D1]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=777039 QWI19_RS12805 WP_009967785.1 2479710..2480120(-) (nucA/comI) [Bacillus subtilis strain 6D1]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCTGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGCTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGAGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTACGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529