Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   PIB33_RS14850 Genome accession   NZ_CP116391
Coordinates   2795768..2798128 (-) Length   786 a.a.
NCBI ID   WP_032722256.1    Uniprot ID   -
Organism   Bacillus subtilis strain GXD-20     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2790768..2803128
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PIB33_RS14830 (PIB33_14830) yrvJ 2790842..2792398 (+) 1557 WP_032722255.1 SH3 domain-containing protein -
  PIB33_RS14835 (PIB33_14835) dtd 2792425..2792865 (-) 441 WP_004398689.1 D-aminoacyl-tRNA deacylase -
  PIB33_RS14840 (PIB33_14840) relA 2792878..2795082 (-) 2205 WP_003229747.1 GTP diphosphokinase -
  PIB33_RS14845 (PIB33_14845) aptA 2795250..2795762 (-) 513 WP_003229745.1 adenine phosphoribosyltransferase -
  PIB33_RS14850 (PIB33_14850) recJ 2795768..2798128 (-) 2361 WP_032722256.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  PIB33_RS14855 (PIB33_14855) yrvD 2798195..2798518 (-) 324 WP_003229740.1 lipopolysaccharide assembly LapA domain-containing protein -
  PIB33_RS14860 (PIB33_14860) yrvC 2798594..2799091 (-) 498 WP_003229739.1 cation:proton antiporter regulatory subunit -
  PIB33_RS14865 (PIB33_14865) secDF 2799249..2801462 (-) 2214 WP_015714442.1 protein translocase subunit SecDF -
  PIB33_RS14870 (PIB33_14870) comN 2801501..2801797 (-) 297 WP_003229735.1 post-transcriptional regulator ComN -

Sequence

Protein


Download         Length: 786 a.a.        Molecular weight: 87981.61 Da        Isoelectric Point: 5.1340

>NTDB_id=777036 PIB33_RS14850 WP_032722256.1 2795768..2798128(-) (recJ) [Bacillus subtilis strain GXD-20]
MLASKMRWEIQRPDQDKVKSLTEQLHITPLVASLLVKRGFDTAESARLFLHTKDADFYDPFEMKGMKEAAYRIKQAISQQ
EKIMIYGDYDADGVTSTSVMLHTLQKLSAQVDFYIPDRFKEGYGPNEQAFRSIKERGFSLIITVDTGIAAVHEAKVAKEL
GLDVIITDHHEPGPELPDVHAIVHPKQPGCTYPFKELAGVGVAFKLAHALLGELPDELLDLAAIGTIADLVPLHDENRLI
ATLGLERLRRTNRLGLKELIKLSGGDIGEANEETVGFQLAPRLNAVGRIEQADPAVHLLMSEDSFEAEELAAEIDQLNKE
RQKMVSKMTDEAIEMVEQQGLDQTAIVVAKAGWNPGVVGIVASKLVDRFYRPAIVLGIDEEKGIAKGSARSIRGFNLFES
LSECRDILPHFGGHPMAAGMTLKAEDVPDLRSRLNEIADNTLTEEDFIPVQEVDLVCGVEDITVESIAEMNMLSPFGMLN
PKPHVLVENAVLEDVRKIGANKTHVKMTIRNESSQLDCVGFNKGELEEGIVPGSRISIVGEMSINEWNNRKKPQLMIKDA
AVSEWQLFDLRGKRTWEDTVSALPSAKRAIVSFKEDSSTLLQTEGLRREVHVISSEDQAKAFDLDGAYIVLLDPPPSLDM
LARLLEGKAPERIYFIFLNHEDHFLSTFPARDHFKWYYAFLLKRGAFDVKKHGSELAKHKGWSVETINFMTKVFFDLGFV
KIENGVLSVVSGAKKRDLTDSQTYQAKQQLMELDQKLNYSSAEELKEWLNKLMKQDSEAYESTRRT

Nucleotide


Download         Length: 2361 bp        

>NTDB_id=777036 PIB33_RS14850 WP_032722256.1 2795768..2798128(-) (recJ) [Bacillus subtilis strain GXD-20]
ATGTTAGCGTCAAAAATGCGATGGGAAATCCAGCGACCAGATCAGGATAAGGTCAAATCACTCACAGAACAATTGCATAT
AACACCTCTTGTTGCGTCCCTGCTTGTAAAAAGAGGCTTTGATACGGCAGAAAGCGCTAGGCTGTTTTTACATACAAAGG
ATGCCGATTTTTATGATCCTTTTGAAATGAAAGGCATGAAAGAAGCGGCATATCGAATAAAACAGGCGATTTCACAACAA
GAAAAGATTATGATATATGGCGATTATGACGCCGATGGGGTTACAAGTACGTCAGTGATGCTTCACACGCTGCAAAAACT
GTCGGCTCAAGTTGATTTTTATATACCTGACCGTTTTAAAGAAGGATATGGACCTAATGAACAGGCGTTTCGTTCGATTA
AAGAACGGGGCTTCTCTTTGATCATTACGGTTGATACAGGAATTGCCGCTGTGCATGAAGCGAAGGTGGCAAAGGAACTC
GGACTAGATGTCATCATTACCGATCATCATGAACCGGGACCGGAGCTTCCGGATGTCCATGCGATCGTCCACCCGAAGCA
GCCTGGCTGCACTTACCCGTTTAAGGAGCTTGCCGGGGTTGGAGTCGCGTTTAAGCTTGCCCACGCTTTATTGGGTGAAC
TTCCTGATGAACTGCTTGACCTGGCGGCTATCGGCACCATTGCCGATCTAGTCCCGCTGCATGACGAAAACAGATTAATT
GCAACACTAGGACTTGAAAGGCTTCGACGGACAAATCGGCTCGGCTTAAAAGAGCTGATTAAGCTGTCAGGCGGAGACAT
CGGAGAAGCGAACGAAGAAACTGTCGGATTTCAGCTCGCACCAAGGCTGAATGCCGTTGGAAGAATCGAACAGGCGGATC
CCGCTGTTCATTTGCTTATGTCAGAGGATTCGTTTGAAGCGGAAGAGCTTGCCGCAGAAATTGACCAGCTGAACAAAGAG
CGCCAAAAAATGGTCAGCAAAATGACGGATGAAGCGATTGAAATGGTTGAACAGCAAGGGCTCGATCAAACTGCAATTGT
CGTGGCCAAAGCCGGATGGAACCCGGGAGTCGTCGGGATTGTGGCTTCAAAGCTTGTGGACCGTTTCTACCGACCGGCCA
TTGTTCTTGGCATTGATGAAGAAAAGGGAATCGCTAAAGGTTCGGCCCGAAGCATCAGAGGATTTAATTTATTTGAGAGC
CTTTCTGAATGCAGAGATATTCTTCCTCATTTCGGCGGCCATCCAATGGCTGCGGGGATGACGCTGAAAGCCGAAGATGT
GCCGGATTTGCGAAGCAGGCTGAATGAAATAGCGGACAATACGCTTACGGAAGAGGATTTTATTCCCGTACAGGAAGTCG
ACCTTGTCTGCGGCGTCGAAGACATTACAGTTGAAAGCATCGCAGAAATGAACATGCTGTCTCCTTTTGGCATGCTGAAC
CCGAAACCGCACGTTCTCGTAGAAAACGCAGTATTGGAAGATGTCCGCAAGATAGGGGCAAATAAAACTCATGTCAAAAT
GACGATCAGAAACGAGTCGTCCCAGCTTGATTGCGTCGGTTTTAATAAAGGCGAGCTTGAAGAAGGCATCGTACCGGGTT
CAAGAATTTCCATTGTCGGTGAGATGTCAATCAACGAATGGAACAATCGAAAAAAGCCGCAGCTTATGATAAAGGATGCC
GCTGTTTCTGAATGGCAGCTGTTTGATTTAAGGGGAAAACGGACGTGGGAAGACACAGTTTCCGCCCTTCCATCTGCTAA
ACGGGCCATTGTCTCTTTCAAAGAAGACTCATCAACATTACTTCAAACTGAAGGCCTTCGCCGAGAAGTGCACGTCATCA
GTTCTGAAGATCAAGCAAAGGCGTTTGACCTTGATGGTGCATATATTGTATTACTGGATCCGCCGCCTTCGCTGGACATG
CTCGCACGTTTGCTGGAAGGCAAGGCGCCTGAGCGGATATATTTTATTTTTCTCAATCATGAGGACCACTTTTTATCAAC
TTTTCCCGCAAGAGACCACTTTAAATGGTATTATGCGTTCTTGCTGAAAAGAGGCGCTTTTGACGTGAAAAAACACGGTT
CAGAGCTTGCAAAACATAAAGGCTGGTCCGTTGAAACAATCAATTTCATGACAAAGGTGTTTTTTGATCTCGGTTTTGTT
AAAATAGAGAATGGTGTGCTGTCTGTTGTGAGCGGAGCAAAGAAACGCGACTTAACAGACTCACAAACATATCAGGCGAA
ACAGCAGCTGATGGAACTGGATCAAAAGCTGAATTATTCTTCTGCTGAAGAGCTGAAGGAATGGCTGAATAAGCTGATGA
AACAAGATTCAGAGGCTTATGAAAGTACGAGGAGGACATAA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(315-437)

DHH family (DHH)

(82-226)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-769)

RecJ OB domain (RecJ_OB)

(453-559)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

99.237

100

0.992