Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   PIB33_RS01985 Genome accession   NZ_CP116391
Coordinates   371724..372173 (-) Length   149 a.a.
NCBI ID   WP_003234608.1    Uniprot ID   A0A8E0S750
Organism   Bacillus subtilis strain GXD-20     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 366724..377173
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PIB33_RS01950 (PIB33_01950) yckA 366999..367679 (-) 681 WP_003234624.1 amino acid ABC transporter permease -
  PIB33_RS01955 (PIB33_01955) yckB 367689..368552 (-) 864 WP_029727074.1 transporter substrate-binding domain-containing protein -
  PIB33_RS01960 (PIB33_01960) - 368714..368906 (+) 193 Protein_342 hypothetical protein -
  PIB33_RS01965 (PIB33_01965) - 368942..369262 (+) 321 Protein_343 RDD family protein -
  PIB33_RS01970 (PIB33_01970) yckD 369344..369676 (+) 333 WP_015252849.1 YckD family protein -
  PIB33_RS01975 (PIB33_01975) bglC 369829..371262 (+) 1434 WP_032722901.1 6-phospho-beta-glucosidase -
  PIB33_RS01980 (PIB33_01980) nin/comJ 371299..371697 (-) 399 WP_032722902.1 DNA-entry nuclease inhibitor Regulator
  PIB33_RS01985 (PIB33_01985) nucA/comI 371724..372173 (-) 450 WP_003234608.1 DNA-entry nuclease Machinery gene
  PIB33_RS01990 (PIB33_01990) tlpC 372341..374062 (-) 1722 WP_029727077.1 methyl-accepting chemotaxis protein TlpC -
  PIB33_RS01995 (PIB33_01995) hxlB 374173..374730 (-) 558 WP_003234605.1 6-phospho-3-hexuloisomerase -
  PIB33_RS02000 (PIB33_02000) hxlA 374736..375368 (-) 633 WP_032722903.1 3-hexulose-6-phosphate synthase -
  PIB33_RS02005 (PIB33_02005) hxlR 375601..375963 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16470.42 Da        Isoelectric Point: 4.5282

>NTDB_id=776988 PIB33_RS01985 WP_003234608.1 371724..372173(-) (nucA/comI) [Bacillus subtilis strain GXD-20]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSTDQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=776988 PIB33_RS01985 WP_003234608.1 371724..372173(-) (nucA/comI) [Bacillus subtilis strain GXD-20]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTTTCAACTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGACACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98