Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BTHE68_RS01225 Genome accession   NZ_AP022315
Coordinates   241592..243109 (-) Length   505 a.a.
NCBI ID   WP_162064790.1    Uniprot ID   -
Organism   Burkholderia sp. THE68     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 236592..248109
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BTHE68_RS01200 (BTHE68_02360) - 237371..238003 (-) 633 WP_061172710.1 LON peptidase substrate-binding domain-containing protein -
  BTHE68_RS01205 (BTHE68_02370) rapZ 238063..238935 (-) 873 WP_162064787.1 RNase adapter RapZ -
  BTHE68_RS01210 (BTHE68_02380) hprK 239018..239986 (-) 969 WP_061172712.1 HPr(Ser) kinase/phosphatase -
  BTHE68_RS01215 (BTHE68_02390) ptsN 240174..240665 (-) 492 WP_353622641.1 PTS IIA-like nitrogen regulatory protein PtsN -
  BTHE68_RS01220 (BTHE68_02400) raiA 241048..241407 (-) 360 WP_162064789.1 ribosome-associated translation inhibitor RaiA -
  BTHE68_RS01225 (BTHE68_02410) rpoN/rpoN1 241592..243109 (-) 1518 WP_162064790.1 RNA polymerase factor sigma-54 Machinery gene
  BTHE68_RS01230 (BTHE68_02420) lptB 243262..244029 (-) 768 WP_174257975.1 LPS export ABC transporter ATP-binding protein -
  BTHE68_RS01235 (BTHE68_02430) lptA 244041..244727 (-) 687 WP_162064792.1 lipopolysaccharide transport periplasmic protein LptA -
  BTHE68_RS01240 (BTHE68_02440) lptC 244758..245360 (-) 603 WP_061172717.1 LPS export ABC transporter periplasmic protein LptC -
  BTHE68_RS01245 (BTHE68_02450) - 245365..245904 (-) 540 WP_061172718.1 HAD family hydrolase -
  BTHE68_RS01250 (BTHE68_02460) kdsD 245904..246887 (-) 984 WP_162064793.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 505 a.a.        Molecular weight: 55454.49 Da        Isoelectric Point: 4.8796

>NTDB_id=77691 BTHE68_RS01225 WP_162064790.1 241592..243109(-) (rpoN/rpoN1) [Burkholderia sp. THE68]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAIAQNPLLENEDDWIASPLRVAADGSLIASPATQNAPSESM
MSNGSSSSTSSSERSESSEPQGVDEYNGMGSDSGQDSSSWNLEDYGRSNNASDDDDLPPLQIHESTTTLRDHLTAQLRMT
AANQRDRALVTFLIESLDEDGYLTSSLDEVQMDLPEELEVDLDELSAALALLQSFDPPGVGGRSASECLKLQLLRLDGSA
TRTLALEIVTNHLELLAARDFTRLRKQLKAGDDALRDAHVLIKSLEPFPGAAYGKSEADYVVPDILVRKSASGWTAELNP
EIVPKLRINHLYANILRNNRGDPGSGSLRQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIA
DTLGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKTPLSDSRIAELLAEQGF
VVARRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1518 bp        

>NTDB_id=77691 BTHE68_RS01225 WP_162064790.1 241592..243109(-) (rpoN/rpoN1) [Burkholderia sp. THE68]
ATGAAAGCCAGCCTCCAACTCCGCCTCTCGCAGCATCTTGCGCTGACCCCGCAACTTCAGCAGTCGATCCGGCTGCTGCA
GTTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGATGGCCATCGCCCAGAATCCGCTGCTCGAGAACGAGGACGACTGGA
TCGCGAGTCCGCTGCGCGTTGCCGCGGACGGATCGCTGATCGCATCGCCGGCGACGCAGAACGCGCCGTCCGAGTCGATG
ATGAGCAATGGCTCGTCGTCGAGCACGTCCAGTTCCGAACGTTCGGAAAGCAGCGAACCACAGGGCGTCGACGAATACAA
CGGCATGGGTTCGGACAGCGGCCAGGATTCGAGTTCATGGAATCTCGAAGACTACGGCCGCTCGAACAACGCCTCCGACG
ACGACGATCTCCCGCCGCTGCAAATCCACGAATCGACCACGACGCTGCGCGACCATCTGACGGCGCAGTTGCGCATGACC
GCGGCGAATCAGCGCGATCGCGCGCTCGTCACGTTTCTGATCGAATCGCTAGACGAAGACGGTTATCTGACTTCATCGCT
CGATGAGGTTCAGATGGATCTGCCCGAGGAACTCGAAGTCGATCTCGACGAACTCAGCGCAGCGCTTGCGTTGCTGCAAA
GTTTCGATCCGCCCGGTGTGGGCGGGCGCTCGGCATCGGAATGTCTGAAGCTGCAGTTGCTTCGGCTCGACGGCTCGGCC
ACGCGCACGCTCGCGCTCGAGATCGTCACGAACCATCTGGAACTGCTCGCCGCGCGTGACTTCACGCGGCTGCGCAAACA
ACTGAAGGCCGGCGACGACGCCCTGCGCGACGCGCATGTTCTGATCAAGTCGCTGGAGCCGTTTCCGGGCGCCGCGTACG
GCAAGAGCGAAGCGGACTACGTGGTGCCGGACATTCTCGTGCGCAAGAGCGCGAGCGGCTGGACGGCGGAGCTGAATCCG
GAGATCGTGCCGAAGCTGCGGATCAACCATCTGTACGCGAATATCCTGCGCAACAATCGTGGAGACCCTGGCAGCGGTTC
ACTGCGTCAGCAGCTTCAGGAAGCGCGCTGGCTCATCAAGAACATTCAGCAGCGGTTCGAGACGATCCTGCGTGTCGCGC
AGGCCATTGTGGAGCGTCAAAAGAACTTCTTTGCACATGGTGAAATCGCCATGCGGCCCTTGGTTTTGCGGGAGATTGCT
GATACGCTGGGATTACACGAATCGACGGTTTCTCGTGTGACGACAGGGAAGTACATGCTCACCCCATTCGGGACGCTTGA
ATTTAAGTACTTCTTTGGATCGCACGTTTCGACCGACACGGGAGGCGCGGCATCGTCCACGGCGATCCGGGCCCTCATCA
AGCAACTGATAGGAGCGGAAGACCCCAAAACTCCTCTTTCAGACAGCCGCATCGCCGAACTGCTGGCGGAACAAGGATTC
GTGGTCGCGCGGCGCACCGTCGCCAAGTACCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTATGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(348-503)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(142-332)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

65.941

100

0.659

Multiple sequence alignment