Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   BTHE68_RS01130 Genome accession   NZ_AP022315
Coordinates   225249..226505 (-) Length   418 a.a.
NCBI ID   WP_162064777.1    Uniprot ID   -
Organism   Burkholderia sp. THE68     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 220249..231505
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BTHE68_RS01110 (BTHE68_02190) ybiB 221918..222919 (-) 1002 WP_162066733.1 DNA-binding protein YbiB -
  BTHE68_RS01115 (BTHE68_02200) - 223232..223930 (+) 699 WP_162066734.1 fumarylacetoacetate hydrolase family protein -
  BTHE68_RS01120 (BTHE68_02210) maiA 223941..224585 (+) 645 WP_162064775.1 maleylacetoacetate isomerase -
  BTHE68_RS01125 (BTHE68_02220) - 224582..225214 (+) 633 WP_162064776.1 LysE/ArgO family amino acid transporter -
  BTHE68_RS01130 (BTHE68_02230) pilA 225249..226505 (-) 1257 WP_162064777.1 signal recognition particle-docking protein FtsY Machinery gene
  BTHE68_RS01135 (BTHE68_02240) rsmD 226739..227365 (+) 627 WP_174257974.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  BTHE68_RS01140 (BTHE68_02250) coaD 227466..227972 (+) 507 WP_061172699.1 pantetheine-phosphate adenylyltransferase -
  BTHE68_RS01145 (BTHE68_02260) - 228050..228310 (+) 261 WP_162064778.1 YfhL family 4Fe-4S dicluster ferredoxin -
  BTHE68_RS01150 (BTHE68_02270) hisC 228307..229374 (-) 1068 WP_162064779.1 histidinol-phosphate transaminase -
  BTHE68_RS01155 (BTHE68_02280) pth 229388..229990 (-) 603 WP_162064780.1 aminoacyl-tRNA hydrolase -
  BTHE68_RS01160 (BTHE68_02290) - 230096..230713 (-) 618 WP_061172703.1 50S ribosomal protein L25/general stress protein Ctc -

Sequence

Protein


Download         Length: 418 a.a.        Molecular weight: 45039.32 Da        Isoelectric Point: 4.4990

>NTDB_id=77690 BTHE68_RS01130 WP_162064777.1 225249..226505(-) (pilA) [Burkholderia sp. THE68]
MFSFFKKFKKPADAPLDTPQEDIVEADGDDDISDAELERQALSAEAPLEEALPEDIQPTEPAPAEPVAPVAPAPAAPVQL
PAGDSKEYWQGRTQSQWARGPETDQASEEIVPPPTLDAVAKKSWLSRLRHGLSKTSSNLTGIFVSAKIDEDLYEELETAL
LMSDAGVDATEFLLESLREKVRSERLTDASQVKAALRELLVDLLRPLEKSLMLGRAQPLVMMIAGVNGVGKTTSIGKLAK
HLQRFDQSVLLAAGDTFRAAAREQLTVWGERNNVTVIAQESGDAAAVIYDAVGAARARKIDVMMADTAGRLPTQLHLMEE
LRKVRRVIAKAMPDAPHEVLLVIDANTGQNALAQVKAFDDALGLTGLVVTKLDGTAKGGILAAIARQRPVPVYFIGVGEK
VEDLQPFSAEEFADALLG

Nucleotide


Download         Length: 1257 bp        

>NTDB_id=77690 BTHE68_RS01130 WP_162064777.1 225249..226505(-) (pilA) [Burkholderia sp. THE68]
ATGTTCAGCTTCTTCAAGAAATTCAAAAAGCCGGCTGACGCGCCGCTCGACACGCCTCAGGAAGACATCGTCGAGGCGGA
CGGGGACGACGACATCTCGGACGCCGAGCTGGAACGGCAGGCGCTCAGCGCTGAAGCCCCACTGGAAGAAGCGCTGCCGG
AGGATATTCAGCCGACGGAACCGGCGCCTGCGGAACCCGTTGCGCCTGTCGCGCCCGCTCCGGCTGCGCCCGTCCAGCTT
CCGGCCGGGGATTCGAAGGAATACTGGCAAGGCCGCACGCAGTCGCAATGGGCGCGCGGTCCGGAAACGGATCAGGCGAG
CGAAGAAATCGTGCCGCCGCCCACGCTTGATGCGGTCGCGAAGAAGTCGTGGCTCTCGCGCCTGCGTCACGGTCTGTCGA
AGACGAGTTCCAACCTGACCGGCATTTTCGTCAGCGCGAAGATCGATGAAGACCTGTACGAAGAGCTCGAAACCGCGCTG
CTGATGTCCGACGCCGGCGTCGATGCCACCGAATTCCTGCTCGAATCGCTGCGCGAAAAAGTGCGCTCGGAGCGTCTGAC
CGATGCATCGCAGGTGAAGGCCGCGCTGCGCGAATTACTCGTCGACCTGCTGCGTCCGCTCGAAAAATCACTGATGCTCG
GCCGCGCGCAACCGCTCGTGATGATGATCGCGGGCGTGAACGGCGTCGGCAAGACGACCAGCATCGGCAAGCTGGCGAAG
CATCTGCAGCGCTTCGATCAGTCGGTGCTGCTCGCCGCCGGCGACACCTTCCGCGCCGCCGCGCGCGAACAGCTCACCGT
CTGGGGCGAGCGCAACAACGTGACCGTGATCGCGCAGGAAAGCGGCGATGCCGCCGCCGTGATTTACGACGCGGTCGGCG
CGGCGCGCGCGCGCAAGATCGACGTGATGATGGCCGACACCGCCGGCCGTCTGCCGACGCAATTGCATCTGATGGAAGAG
CTGCGCAAGGTGCGCCGCGTGATCGCGAAGGCGATGCCCGACGCGCCGCACGAAGTGCTGCTCGTGATCGACGCCAACAC
GGGTCAGAACGCGCTCGCGCAGGTCAAGGCGTTCGACGATGCGCTGGGCCTGACCGGTCTCGTCGTCACGAAACTCGACG
GCACCGCGAAAGGCGGCATTCTCGCCGCGATCGCGCGACAGCGGCCGGTGCCGGTGTATTTCATCGGCGTGGGCGAGAAG
GTCGAGGATCTGCAACCGTTCAGCGCCGAAGAATTCGCCGACGCGCTGCTCGGCTGA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(138-198)

SRP54-type protein, GTPase domain (SRP54)

(219-418)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

50.469

100

0.514

Multiple sequence alignment