Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PK654_RS00890 Genome accession   NZ_CP116383
Coordinates   191616..192956 (+) Length   446 a.a.
NCBI ID   WP_271697067.1    Uniprot ID   -
Organism   Vibrio sp. SCSIO 43137     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 186616..197956
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PK654_RS00870 (PK654_00870) pilM 189345..189962 (+) 618 WP_271697063.1 pilus assembly protein PilM -
  PK654_RS00875 (PK654_00875) - 189952..190527 (+) 576 WP_271697064.1 PilN domain-containing protein -
  PK654_RS00880 (PK654_00880) pilO 190505..191089 (+) 585 WP_271697065.1 type 4a pilus biogenesis protein PilO -
  PK654_RS00885 (PK654_00885) - 191086..191619 (+) 534 WP_271697066.1 pilus assembly protein PilP -
  PK654_RS00890 (PK654_00890) pilQ 191616..192956 (+) 1341 WP_271697067.1 type IV pilus secretin PilQ Machinery gene
  PK654_RS00895 (PK654_00895) aroK 193183..193701 (+) 519 WP_271697069.1 shikimate kinase AroK -
  PK654_RS00900 (PK654_00900) aroB 193735..194820 (+) 1086 WP_271697070.1 3-dehydroquinate synthase -
  PK654_RS00905 (PK654_00905) - 194856..196448 (+) 1593 WP_271697072.1 AAA family ATPase -
  PK654_RS00910 (PK654_00910) - 196517..197380 (+) 864 WP_271697073.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 446 a.a.        Molecular weight: 48444.69 Da        Isoelectric Point: 4.7991

>NTDB_id=776844 PK654_RS00890 WP_271697067.1 191616..192956(+) (pilQ) [Vibrio sp. SCSIO 43137]
MRANICQRLVLMVLLILSFAVKAEQGAQLSEPDKRISMYFQDVPVTEALQQLALLNSTNLVISDSVSGHVSVKLQDVPWQ
QAFDAILSLKALQAEKQGDILLVAPENELRAQEKLRLESLEQAILLEPLTMDSFTIKHTSAENLASVIDGEKGVSLLSSR
GSVTVDSRTNTLLIKDIAENIAAIQKMIKTLDRPVKQVQIEARIVSMNEGQLDELGVRWGVSSDRGDIKAGGTIEGVSLE
DADLSDRLNVSFAATSVKAASAAFQLATLGQGVLLDLELSALQAESKAEIISSPRLITTNKTPAYIEQGTEIPYLEPGAN
DATSISFKKAVLSLEVTPQIIENNQLILDLLISQDRPGEVVKSGTGEAVAIDTQRVKTQVRLNNEQTLVLGGVLHYSNIR
QTDKVPLLGDIPAIGHLFKTKYEKITKRELLIFVTPKVIISGGNVE

Nucleotide


Download         Length: 1341 bp        

>NTDB_id=776844 PK654_RS00890 WP_271697067.1 191616..192956(+) (pilQ) [Vibrio sp. SCSIO 43137]
ATGAGGGCTAATATCTGTCAGCGTTTAGTTTTAATGGTTCTGCTGATTTTGAGCTTTGCTGTTAAAGCAGAGCAAGGTGC
ACAACTTTCAGAGCCTGATAAGCGAATTTCAATGTATTTTCAGGATGTGCCTGTAACAGAAGCCCTGCAACAATTGGCAC
TGTTGAACAGTACCAATCTGGTTATCTCTGATAGTGTCAGTGGGCATGTGTCCGTTAAGCTACAAGACGTTCCCTGGCAA
CAGGCGTTTGACGCTATCCTCAGCCTGAAAGCTCTGCAGGCTGAAAAGCAGGGAGATATTTTGCTGGTGGCGCCGGAAAA
TGAACTACGTGCTCAGGAGAAGCTCAGGCTAGAGTCACTGGAGCAGGCTATCTTGCTGGAACCGCTGACTATGGACAGTT
TTACTATCAAGCATACTAGTGCAGAAAATCTTGCAAGTGTTATTGATGGAGAGAAAGGGGTTTCATTGCTCTCTTCGAGA
GGGAGCGTAACGGTAGACAGCAGAACCAATACTTTGCTGATAAAAGATATTGCTGAAAATATTGCCGCTATCCAAAAAAT
GATTAAAACCTTAGACAGGCCGGTAAAGCAGGTGCAGATTGAAGCGCGAATTGTTTCAATGAATGAGGGACAACTTGATG
AGTTGGGTGTGCGCTGGGGGGTTAGCTCTGACAGAGGCGATATCAAAGCTGGCGGAACCATAGAAGGAGTTAGTTTAGAG
GATGCTGACTTGTCAGACAGGCTAAATGTCAGCTTTGCGGCAACATCCGTTAAAGCGGCCAGTGCAGCATTTCAGCTTGC
TACTTTAGGGCAGGGTGTTTTGCTGGATCTAGAGTTGTCGGCACTGCAGGCTGAATCCAAAGCCGAGATAATTTCCAGCC
CGCGGCTGATTACCACTAACAAAACACCAGCTTATATAGAACAAGGTACAGAGATCCCTTATCTGGAGCCTGGAGCTAAC
GATGCAACGTCAATATCCTTCAAAAAAGCGGTATTGAGTTTGGAAGTCACGCCACAAATTATCGAAAATAATCAGCTAAT
ATTGGATCTTTTAATCTCGCAGGACAGGCCGGGAGAGGTAGTAAAGAGCGGCACAGGAGAGGCCGTTGCCATTGATACCC
AGAGAGTAAAAACACAAGTCAGGTTGAACAATGAACAAACCTTAGTGCTTGGAGGAGTGCTGCATTACAGCAATATAAGA
CAGACAGATAAAGTACCCTTGTTAGGGGATATTCCCGCAATAGGTCATTTATTTAAAACAAAATACGAAAAAATAACAAA
GAGAGAGCTACTGATATTTGTTACGCCAAAGGTGATTATATCTGGTGGCAATGTTGAATAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(133-197)

Bacterial type II and III secretion system protein (Secretin)

(281-439)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

54.378

97.309

0.529
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

55.37

93.946

0.52
  pilQ Vibrio cholerae strain A1552

55.37

93.946

0.52
  comE Haemophilus influenzae Rd KW20

41.289

93.946

0.388
  pilQ Legionella pneumophila strain ERS1305867

41.247

93.498

0.386
  pilQ Legionella pneumophila strain Lp02

41.247

93.498

0.386
  comE Haemophilus influenzae 86-028NP

40.732

91.928

0.374
  comQ Acinetobacter baylyi ADP1

37.02

99.327

0.368
  pilQ Neisseria gonorrhoeae strain FA1090

37.617

95.964

0.361