Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   QVM90_RS03575 Genome accession   NZ_CP128979
Coordinates   724924..726417 (+) Length   497 a.a.
NCBI ID   WP_097177885.1    Uniprot ID   -
Organism   Escherichia coli strain TUM1881     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 719924..731417
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QVM90_RS03545 (QVM90_03545) - 720110..720421 (+) 312 Protein_693 inorganic phosphate transporter -
  QVM90_RS03550 (QVM90_03550) - 720477..721174 (+) 698 WP_223274117.1 IS1 family transposase -
  QVM90_RS03555 (QVM90_03555) - 721190..721381 (+) 192 Protein_695 A24 family peptidase -
  QVM90_RS03560 (QVM90_03560) gspS2 721447..721857 (+) 411 WP_001324279.1 type II secretion system pilot lipoprotein GspS-beta -
  QVM90_RS03565 (QVM90_03565) gspC 721932..722834 (+) 903 WP_097177886.1 type II secretion system protein GspC -
  QVM90_RS03570 (QVM90_03570) gspD 722864..724924 (+) 2061 Protein_698 type II secretion system secretin GspD -
  QVM90_RS03575 (QVM90_03575) pilB/pilB1 724924..726417 (+) 1494 WP_097177885.1 type II secretion system ATPase GspE Machinery gene
  QVM90_RS03580 (QVM90_03580) gspF 726417..727640 (+) 1224 WP_001173458.1 type II secretion system inner membrane protein GspF -
  QVM90_RS03585 (QVM90_03585) gspG 727657..728112 (+) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  QVM90_RS03590 (QVM90_03590) gspH 728116..728679 (+) 564 WP_001115140.1 type II secretion system minor pseudopilin GspH -
  QVM90_RS03595 (QVM90_03595) gspI 728676..729047 (+) 372 WP_000820125.1 type II secretion system minor pseudopilin GspI -
  QVM90_RS03600 (QVM90_03600) gspJ 729044..729643 (+) 600 WP_001255040.1 type II secretion system minor pseudopilin GspJ -
  QVM90_RS03605 (QVM90_03605) gspK 729646..730623 (+) 978 WP_000633238.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55205.39 Da        Isoelectric Point: 6.1634

>NTDB_id=776729 QVM90_RS03575 WP_097177885.1 724924..726417(+) (pilB/pilB1) [Escherichia coli strain TUM1881]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEVEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDNAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQAAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=776729 QVM90_RS03575 WP_097177885.1 724924..726417(+) (pilB/pilB1) [Escherichia coli strain TUM1881]
ATGGTGCCTGTAGCACAGGAAACCACCGCCAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCACGTTCATCCGCTATCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAGGTCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCTCGCCAGCTGATGGAAGATCTCGGTTCTGCCGAGGACTTTTTTACCCTCGCGGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGCGACGACAATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGCGTTGACGGCACATTACATGAAATGCTGCGC
CCGGGGCGCAAACTGGCCTCGCTGCTGGTATCGCGTATCAAGGTGATGGCACGGCTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGACGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCCTCCGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGCTTAGCCAGCAACTGACC
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGTAACATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGGATCGGTCAGACACAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCATCGCTTACCGGACACCT
GGTCCTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTCCGCACGCTGTGTCCCGACTGCCGTCAGGCCGCGCCTGCC
ACTGACGAAGAAAAACGCCTGCTGGGCATTACCGATGCGCGAACCGTCACTCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTCCGCGGACGGACTGCCATCCATGAGCTGATCGTGGTGGATGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAGCTGGAGCTGGAACGTTATGTCCGACAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Vibrio parahaemolyticus RIMD 2210633

48.363

79.879

0.386
  pilB Vibrio campbellii strain DS40M4

47.222

79.678

0.376
  pilB Legionella pneumophila strain ERS1305867

43.387

86.72

0.376
  pilB Acinetobacter baumannii D1279779

46.366

80.282

0.372
  pilB Vibrio cholerae strain A1552

46.212

79.678

0.368
  pilF Thermus thermophilus HB27

45.5

80.483

0.366
  pilB Acinetobacter baylyi ADP1

46.154

78.471

0.362