Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PI860_RS06485 Genome accession   NZ_CP116258
Coordinates   1325299..1326738 (-) Length   479 a.a.
NCBI ID   WP_005314410.1    Uniprot ID   A4SHX7
Organism   Aeromonas salmonicida subsp. salmonicida strain J225     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1320299..1331738
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PI860_RS06455 (PI860_06460) dagF 1320562..1321302 (+) 741 WP_005314402.1 2-dehydro-3-deoxy-phosphogluconate aldolase -
  PI860_RS06460 (PI860_06465) - 1321406..1323320 (+) 1915 Protein_1240 BglG family transcription antiterminator -
  PI860_RS06465 (PI860_06470) npr 1323339..1323611 (-) 273 WP_005314403.1 PTS phosphocarrier protein NPr -
  PI860_RS06470 (PI860_06475) rapZ 1323632..1324498 (-) 867 WP_005314405.1 RNase adapter RapZ -
  PI860_RS06475 (PI860_06480) ptsN 1324541..1324987 (-) 447 WP_005314406.1 PTS IIA-like nitrogen regulatory protein PtsN -
  PI860_RS06480 (PI860_06485) hpf 1324990..1325277 (-) 288 WP_005305957.1 ribosome hibernation promoting factor -
  PI860_RS06485 (PI860_06490) rpoN/rpoN1 1325299..1326738 (-) 1440 WP_005314410.1 RNA polymerase factor sigma-54 Machinery gene
  PI860_RS06490 (PI860_06495) lptB 1326804..1327529 (-) 726 WP_005314412.1 LPS export ABC transporter ATP-binding protein -
  PI860_RS06495 (PI860_06500) lptA 1327533..1328060 (-) 528 WP_005314414.1 lipopolysaccharide transport periplasmic protein LptA -
  PI860_RS06500 (PI860_06505) lptC 1328047..1328607 (-) 561 WP_005314417.1 LPS export ABC transporter periplasmic protein LptC -
  PI860_RS06505 (PI860_06510) kdsC 1328604..1329158 (-) 555 WP_005314419.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  PI860_RS06510 (PI860_06515) - 1329158..1330153 (-) 996 WP_011898279.1 KpsF/GutQ family sugar-phosphate isomerase -
  PI860_RS06515 (PI860_06520) mlaF 1330361..1331161 (+) 801 WP_011898278.1 phospholipid ABC transporter ATP-binding protein MlaF -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53962.92 Da        Isoelectric Point: 4.3718

>NTDB_id=776421 PI860_RS06485 WP_005314410.1 1325299..1326738(-) (rpoN/rpoN1) [Aeromonas salmonicida subsp. salmonicida strain J225]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENDVQETSPEEPVDDSQLDSSDAMAQDQMP
DELPVDTTWEDVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDLDAAIALAIIDAIDDSGYLTVSLED
IQAAVSSEDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLGQYAPELPWLNEAREVIKEHMDLLGNRDYRTLARK
TKLKESDLKEVLDLIQQLEPRPGNGIIQSDSQYVIPDVTVVKKGNKWVVELNPDSAPRIRVNETYAAMARQARTSQDTQF
IRSHLQEAKWFIKSLESRNDTLLKVATCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGRECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=776421 PI860_RS06485 WP_005314410.1 1325299..1326738(-) (rpoN/rpoN1) [Aeromonas salmonicida subsp. salmonicida strain J225]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGATAACAATCCTTTGCTTGAGCAAGAGGAGAATGACG
TTCAGGAGACCAGCCCGGAAGAGCCCGTCGACGACAGTCAGCTCGACTCCAGCGATGCCATGGCCCAGGATCAGATGCCG
GACGAACTGCCGGTAGATACCACCTGGGAAGATGTCTATTCAGCCGGCACAGCCCAGAGCGCAGGCCCCATCCACGATGG
GGAGGACAGCGTCTATCAAGGGGAAACCACCGAGAACCTGCAGGACTATCTGCTGTGGCAGATGCGCCTCACCCCGTTCA
GCGATCTGGATGCCGCCATTGCGCTGGCCATCATAGATGCCATCGATGACTCCGGTTATCTGACCGTCTCTCTCGAAGAT
ATACAGGCGGCGGTGAGCAGCGAGGATGTCGAGGTGGAACTCGACGAGGTCGAGGCAGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTCGGCATCGCCGCGCGCTCGGTGCAGGAGTGTCTGCTGATCCAGCTTGGCCAGTACGCTCCTGAACTGCCCT
GGCTCAATGAGGCCAGGGAGGTAATCAAGGAGCACATGGACTTGCTCGGCAACCGTGATTACCGCACCCTGGCCCGCAAG
ACCAAGCTCAAGGAGAGTGACCTCAAGGAGGTGCTGGACCTTATCCAGCAACTGGAGCCCAGGCCGGGTAACGGCATCAT
ACAGAGTGACTCCCAGTACGTGATCCCGGATGTCACCGTCGTCAAGAAGGGCAACAAATGGGTCGTTGAGCTCAACCCTG
ATTCTGCACCGCGCATCCGGGTCAACGAGACCTATGCCGCCATGGCCCGTCAGGCGCGTACCAGCCAGGATACCCAGTTC
ATTCGCTCCCACTTGCAAGAAGCCAAGTGGTTCATCAAGAGTCTGGAGAGTCGCAACGACACCCTGCTCAAGGTGGCGAC
CTGTATTGTCGAGCAACAACAGGGCTTTTTCGAGTATGGTGAAGAAGCCATGAAGCCCATGGTGCTCAACGATATCGCCG
AGGCGGTGGAGATGCATGAGTCGACCATTTCCAGGGTAACGACCCAGAAATACATGCATACCCCCCGCGGCATCTTCGAA
CTGAAGTTTTTTTTCTCCAGTCATGTGAACACAGAAGGAGGAAGAGAGTGCTCATCGACCGCAATTCGCGCGCTGATCAA
GAAGCTGGTGACGGCGGAGAATCCCGCCAAGCCGCTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A4SHX7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.58

100

0.484