Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   H7R46_RS01540 Genome accession   NZ_AP022302
Coordinates   325957..326736 (+) Length   259 a.a.
NCBI ID   WP_005067082.1    Uniprot ID   -
Organism   Acinetobacter sp. BEC1-S18-ESBL-01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 320957..331736
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7R46_RS01525 (BE1S18E01_02910) ponA 321538..324099 (-) 2562 WP_005067076.1 penicillin-binding protein PBP1a -
  H7R46_RS01530 (BE1S18E01_02920) pilM 324261..325319 (+) 1059 WP_005067078.1 pilus assembly protein PilM Machinery gene
  H7R46_RS01535 (BE1S18E01_02930) pilN 325319..325960 (+) 642 WP_005067080.1 PilN domain-containing protein Machinery gene
  H7R46_RS01540 (BE1S18E01_02940) pilO 325957..326736 (+) 780 WP_005067082.1 type 4a pilus biogenesis protein PilO Machinery gene
  H7R46_RS01545 (BE1S18E01_02950) pilP 326748..327275 (+) 528 WP_005067086.1 pilus assembly protein PilP Machinery gene
  H7R46_RS01550 (BE1S18E01_02960) pilQ 327338..329464 (+) 2127 WP_005067089.1 type IV pilus secretin PilQ family protein Machinery gene
  H7R46_RS01555 (BE1S18E01_02970) aroK 329476..330045 (+) 570 WP_002116987.1 shikimate kinase AroK -
  H7R46_RS01560 (BE1S18E01_02980) aroB 330074..331156 (+) 1083 WP_005067096.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 259 a.a.        Molecular weight: 29421.52 Da        Isoelectric Point: 5.7456

>NTDB_id=77623 H7R46_RS01540 WP_005067082.1 325957..326736(+) (pilO) [Acinetobacter sp. BEC1-S18-ESBL-01]
MSPDELHELSLEQAPSKKKKITLEKFLQQFNTLDMNSYGSWPLSVKITCWIFIFFAVLALGYFVAIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQLQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
ITIEATGDYHAFGAFVSSIAALPRIVTMHDFIVEAAPVKDGKSDIPVLNYSIKAKTYRYMGAVENQNNSDPQVINGESQI
NASDSRNAHIPSIKKSKRK

Nucleotide


Download         Length: 780 bp        

>NTDB_id=77623 H7R46_RS01540 WP_005067082.1 325957..326736(+) (pilO) [Acinetobacter sp. BEC1-S18-ESBL-01]
ATGAGTCCTGATGAACTTCATGAGCTGTCTTTAGAACAAGCTCCATCTAAGAAAAAAAAGATCACCTTAGAAAAATTTCT
TCAGCAATTTAACACGCTCGACATGAACAGTTATGGCAGCTGGCCATTATCTGTGAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTACTGGCTTTAGGGTATTTTGTTGCTATTCAACCAAAGCTACAAGCAATTGATAATGCTCAAGCACAAGAA
AGTAATTTATTAAATGAGTTTCGAGAAAAAGACTCAAAATTACGCAACTTGCAGCAATACCAGCTTCAACTTCAAGAGAT
GCAAGCCAACTTCAATCAACAGTTAGAACAATTGCCAAAAGAAACCGAAATTCCAAGTTTAGTTGAAGATATTAACTTAA
CGGGGGTGAATTCTGGTCTGAAGTTTAAAAATATCCGCTTAGAAGATGAAGTGAAGCAGGAAATTTTTATTGAACAGCCT
ATTACTATCGAAGCAACTGGGGATTATCACGCTTTCGGTGCTTTTGTTAGCAGTATTGCGGCATTACCACGTATTGTGAC
GATGCATGATTTTATTGTTGAAGCTGCTCCTGTTAAGGATGGTAAATCAGATATTCCTGTGCTTAATTATTCTATCAAAG
CAAAAACTTATCGTTATATGGGCGCGGTTGAAAACCAAAATAATAGTGATCCTCAAGTAATAAATGGTGAAAGTCAGATA
AACGCCTCCGATAGCCGTAATGCACATATTCCTTCTATAAAGAAGTCAAAGCGAAAGTAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

89.732

86.486

0.776
  comO Acinetobacter baylyi ADP1

71.983

89.575

0.645

Multiple sequence alignment