Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   PGW89_RS10680 Genome accession   NZ_CP116040
Coordinates   2273190..2273990 (+) Length   266 a.a.
NCBI ID   WP_026056343.1    Uniprot ID   A0AAW4JFL0
Organism   Acinetobacter haemolyticus strain HW-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2268190..2278990
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PGW89_RS10655 (PGW89_10655) - 2269129..2270415 (+) 1287 WP_075314791.1 glycosyltransferase family 1 protein -
  PGW89_RS10660 (PGW89_10660) - 2270439..2270990 (+) 552 WP_004640826.1 phosphatase PAP2 family protein -
  PGW89_RS10665 (PGW89_10665) iscX 2271075..2271275 (+) 201 WP_004640824.1 Fe-S cluster assembly protein IscX -
  PGW89_RS10670 (PGW89_10670) ndk 2271385..2271816 (+) 432 WP_004640823.1 nucleoside-diphosphate kinase -
  PGW89_RS10675 (PGW89_10675) rlmN 2271937..2273172 (+) 1236 WP_005083389.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  PGW89_RS10680 (PGW89_10680) pilF 2273190..2273990 (+) 801 WP_026056343.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  PGW89_RS10685 (PGW89_10685) - 2273981..2274775 (+) 795 WP_004640817.1 helix-turn-helix domain-containing protein -
  PGW89_RS10690 (PGW89_10690) ispG 2274797..2275912 (+) 1116 WP_113996690.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  PGW89_RS10695 (PGW89_10695) hisS 2275909..2277201 (+) 1293 WP_113996691.1 histidine--tRNA ligase -
  PGW89_RS10700 (PGW89_10700) - 2277226..2277924 (+) 699 WP_004640812.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30220.21 Da        Isoelectric Point: 9.6050

>NTDB_id=775512 PGW89_RS10680 WP_026056343.1 2273190..2273990(+) (pilF) [Acinetobacter haemolyticus strain HW-2]
MNTTRLKTTIAITVVLMAGGLTACQSTPSTKDPEKAVQVRTQLAAEYIRTRDLDSAKRSLDQALKLNSRDANANMMMGIL
LQQEGSKLNMEKADAYFKRAISSEPDNAQARNNYGTYLYQMQRYNEAIEQFTRAGATLGYDQRFQSLENLGRIYLLLGDV
TNAEKSFKQALQANRNATISMLELSEIFYLQQNNRDASQLYEQYVRSVGQRNQGARALWIGIRIARANADQLGMQVLVNQ
LRALFPDSPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=775512 PGW89_RS10680 WP_026056343.1 2273190..2273990(+) (pilF) [Acinetobacter haemolyticus strain HW-2]
TTGAATACAACTCGACTAAAAACGACCATAGCGATAACAGTTGTACTTATGGCAGGTGGCTTGACAGCTTGCCAATCGAC
ACCATCTACGAAAGATCCAGAGAAAGCTGTTCAGGTTCGCACCCAATTGGCTGCGGAATATATTCGTACGCGTGATTTGG
ACTCTGCAAAACGTTCACTTGACCAAGCATTGAAACTTAATTCCCGTGATGCAAATGCCAATATGATGATGGGTATTTTG
CTGCAACAAGAGGGAAGTAAGCTCAATATGGAAAAAGCAGATGCTTATTTTAAGCGAGCGATTTCATCTGAGCCAGACAA
TGCTCAAGCGCGTAATAATTACGGTACATACTTATATCAGATGCAGCGTTATAATGAAGCAATTGAGCAGTTCACGCGTG
CTGGTGCAACCTTAGGTTATGATCAACGATTTCAATCATTGGAAAACCTAGGGCGAATCTATTTATTATTGGGCGATGTG
ACGAATGCTGAAAAATCATTCAAACAGGCGCTGCAAGCAAATCGAAATGCAACCATTTCAATGTTAGAGTTGTCGGAAAT
TTTCTATTTGCAACAAAACAATAGAGATGCATCCCAGTTATATGAACAATATGTTCGTAGCGTGGGACAGCGCAACCAAG
GTGCTAGAGCACTTTGGATCGGGATACGCATTGCTCGAGCGAATGCGGATCAGTTAGGAATGCAAGTGTTAGTCAACCAA
TTACGCGCTTTATTTCCTGATAGTCCAGAATATCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_12)

(108-175)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

76.58

100

0.774
  pilF Acinetobacter baylyi ADP1

68.045

100

0.68