Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   PF996_RS01820 Genome accession   NZ_CP116014
Coordinates   350301..350738 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain SRCM124633     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 345301..355738
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PF996_RS01795 (PF996_01795) - 346321..347457 (+) 1137 WP_257988993.1 zinc-dependent alcohol dehydrogenase -
  PF996_RS01800 (PF996_01800) - 347472..347906 (+) 435 WP_014416871.1 RDD family protein -
  PF996_RS01805 (PF996_01805) - 348023..348301 (+) 279 WP_263612330.1 YckD family protein -
  PF996_RS01810 (PF996_01810) - 348405..349841 (+) 1437 WP_014416872.1 glycoside hydrolase family 1 protein -
  PF996_RS01815 (PF996_01815) nin/comJ 349882..350280 (-) 399 WP_017419379.1 competence protein ComJ Regulator
  PF996_RS01820 (PF996_01820) nucA/comI 350301..350738 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  PF996_RS01825 (PF996_01825) hxlB 351069..351626 (-) 558 WP_014416874.1 6-phospho-3-hexuloisomerase -
  PF996_RS01830 (PF996_01830) hxlA 351623..352258 (-) 636 WP_015239130.1 3-hexulose-6-phosphate synthase -
  PF996_RS01835 (PF996_01835) - 352490..352852 (+) 363 WP_007409352.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=775234 PF996_RS01820 WP_003156588.1 350301..350738(-) (nucA/comI) [Bacillus velezensis strain SRCM124633]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=775234 PF996_RS01820 WP_003156588.1 350301..350738(-) (nucA/comI) [Bacillus velezensis strain SRCM124633]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCCGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGTTACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATTAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641