Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   QU519_RS01970 Genome accession   NZ_CP128642
Coordinates   422185..422652 (-) Length   155 a.a.
NCBI ID   WP_000340171.1    Uniprot ID   -
Organism   Escherichia coli strain WL164     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 417185..427652
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QU519_RS01945 (QU519_01945) rpsG 417326..417796 (+) 471 WP_001138043.1 30S ribosomal protein S7 -
  QU519_RS01950 (QU519_01950) fusA 417893..420007 (+) 2115 WP_000124700.1 elongation factor G -
  QU519_RS01955 (QU519_01955) tuf 420078..421262 (+) 1185 WP_000031783.1 elongation factor Tu -
  QU519_RS01960 (QU519_01960) bfd 421445..421639 (+) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  QU519_RS01965 (QU519_01965) bfr 421712..422188 (+) 477 WP_000675515.1 bacterioferritin -
  QU519_RS01970 (QU519_01970) pilD 422185..422652 (-) 468 WP_000340171.1 prepilin peptidase Machinery gene
  QU519_RS01975 (QU519_01975) rpsJ 423031..423342 (+) 312 WP_001181004.1 30S ribosomal protein S10 -
  QU519_RS01980 (QU519_01980) rplC 423375..424004 (+) 630 WP_000579833.1 50S ribosomal protein L3 -
  QU519_RS01985 (QU519_01985) rplD 424015..424620 (+) 606 WP_000424395.1 50S ribosomal protein L4 -
  QU519_RS01990 (QU519_01990) rplW 424617..424919 (+) 303 WP_000617544.1 50S ribosomal protein L23 -
  QU519_RS01995 (QU519_01995) rplB 424937..425758 (+) 822 WP_000301864.1 50S ribosomal protein L2 -
  QU519_RS02000 (QU519_02000) rpsS 425775..426053 (+) 279 WP_001138117.1 30S ribosomal protein S19 -
  QU519_RS02005 (QU519_02005) rplV 426068..426400 (+) 333 WP_000447529.1 50S ribosomal protein L22 -
  QU519_RS02010 (QU519_02010) rpsC 426418..427119 (+) 702 WP_000529945.1 30S ribosomal protein S3 -
  QU519_RS02015 (QU519_02015) rplP 427132..427542 (+) 411 WP_000941212.1 50S ribosomal protein L16 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16938.08 Da        Isoelectric Point: 8.1296

>NTDB_id=775154 QU519_RS01970 WP_000340171.1 422185..422652(-) (pilD) [Escherichia coli strain WL164]
MDATLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=775154 QU519_RS01970 WP_000340171.1 422185..422652(-) (pilD) [Escherichia coli strain WL164]
ATGGACGCTACCCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTGGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.8

80.645

0.361
  pilD Neisseria gonorrhoeae MS11

38.095

94.839

0.361