Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   PF977_RS13825 Genome accession   NZ_CP116012
Coordinates   2628607..2629500 (-) Length   297 a.a.
NCBI ID   WP_015715882.1    Uniprot ID   A0A8E0SFH2
Organism   Bacillus subtilis strain SRCM124333     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2623607..2634500
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PF977_RS13810 (PF977_13810) xerC 2623979..2624893 (-) 915 WP_003231988.1 tyrosine recombinase XerC -
  PF977_RS13815 (PF977_13815) trmFO 2624961..2626268 (-) 1308 WP_015715883.1 FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO -
  PF977_RS13820 (PF977_13820) topA 2626344..2628419 (-) 2076 WP_003245599.1 type I DNA topoisomerase -
  PF977_RS13825 (PF977_13825) dprA 2628607..2629500 (-) 894 WP_015715882.1 DNA-processing protein DprA Machinery gene
  PF977_RS13830 (PF977_13830) sucD 2629561..2630463 (-) 903 WP_003238566.1 succinate--CoA ligase subunit alpha -
  PF977_RS13835 (PF977_13835) sucC 2630492..2631649 (-) 1158 WP_015715881.1 ADP-forming succinate--CoA ligase subunit beta -
  PF977_RS13840 (PF977_13840) ylqH 2631822..2632103 (-) 282 WP_003232001.1 FlhB-like flagellar biosynthesis protein -
  PF977_RS13845 (PF977_13845) - 2632100..2633830 (-) 1731 WP_015715880.1 hypothetical protein -

Sequence

Protein


Download         Length: 297 a.a.        Molecular weight: 32904.21 Da        Isoelectric Point: 9.9142

>NTDB_id=775118 PF977_RS13825 WP_015715882.1 2628607..2629500(-) (dprA) [Bacillus subtilis strain SRCM124333]
MDQAAACLTICRINQLLSPSLLLKWWKADPSMSLTSPVLQTVTRDQIKAAALKNEIEQLYPKLQRVLAAYREQGINTIPI
SSKQYPFWLKSIYDPPAVLFAKGDMTLLSKGRKIGIVGTRNPTAYGKQVVNHLTKEICRKGWVIVSGLASGIDGMSHAAS
IKAKGRTIGVIAGGFQHIYPRENLQLADHMAKHHILLSEHPPETKPQKWHFPMRNRIISGLSEGVIVVQGKEKSGSLITA
YQALEQGREVFAVPGSLFDPYAGGPIKLIQQGAKAIWSAEDIFEELPERNVQYTEPF

Nucleotide


Download         Length: 894 bp        

>NTDB_id=775118 PF977_RS13825 WP_015715882.1 2628607..2629500(-) (dprA) [Bacillus subtilis strain SRCM124333]
TTGGATCAGGCCGCTGCCTGCCTAACGATTTGCAGAATCAATCAATTATTATCCCCATCCCTTCTATTAAAATGGTGGAA
AGCCGATCCGTCTATGTCGCTGACATCACCTGTGTTACAAACGGTTACTCGTGATCAAATAAAAGCAGCTGCATTAAAAA
ACGAAATAGAACAACTTTATCCAAAGCTCCAGCGTGTACTTGCTGCTTATCGTGAGCAAGGCATTAACACCATCCCTATT
TCTTCAAAGCAATATCCTTTCTGGCTTAAAAGCATTTATGATCCCCCCGCCGTACTGTTTGCAAAAGGTGATATGACTCT
TCTTTCGAAAGGGAGAAAAATTGGAATTGTAGGGACAAGAAATCCAACAGCTTATGGGAAACAAGTTGTCAATCATCTTA
CAAAAGAGATCTGTCGTAAAGGTTGGGTGATTGTCAGCGGACTGGCGTCTGGGATAGACGGAATGTCCCATGCTGCAAGT
ATTAAGGCGAAGGGGCGGACAATTGGCGTCATTGCAGGCGGATTTCAGCACATTTATCCCCGAGAAAACCTTCAGTTAGC
AGATCACATGGCTAAACACCATATCCTGCTGTCAGAGCACCCACCTGAAACTAAACCCCAAAAATGGCATTTCCCTATGA
GAAACCGTATTATCAGCGGACTAAGTGAAGGCGTTATTGTCGTTCAGGGCAAAGAAAAAAGCGGTTCGCTGATTACTGCC
TATCAAGCATTGGAACAAGGGAGAGAGGTATTTGCCGTACCCGGTTCATTGTTTGATCCTTACGCCGGAGGTCCTATAAA
ACTGATCCAGCAGGGGGCTAAAGCCATATGGTCAGCAGAGGATATTTTCGAGGAACTTCCTGAGAGAAACGTTCAATATA
CGGAACCCTTTTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(71-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Bacillus subtilis subsp. subtilis str. 168

98.99

100

0.99
  dprA Lactococcus lactis subsp. cremoris KW2

42.105

89.562

0.377