Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   PF977_RS08235 Genome accession   NZ_CP116012
Coordinates   1587516..1587926 (+) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain SRCM124333     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1582516..1592926
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PF977_RS08200 (PF977_08200) arsR 1582681..1582998 (+) 318 WP_029318103.1 arsenical resistance operon transcriptional regulator ArsR -
  PF977_RS08205 (PF977_08205) arsK 1583057..1583497 (+) 441 WP_032722151.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  PF977_RS08210 (PF977_08210) acr3 1583520..1584560 (+) 1041 WP_032722149.1 arsenite efflux transporter Acr3 -
  PF977_RS08215 (PF977_08215) arsC 1584572..1584991 (+) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  PF977_RS08220 (PF977_08220) - 1585346..1585524 (+) 179 Protein_1614 hypothetical protein -
  PF977_RS08225 (PF977_08225) spoIVCA 1585482..1586942 (+) 1461 WP_243737903.1 site-specific DNA recombinase SpoIVCA -
  PF977_RS08230 (PF977_08230) - 1586970..1587320 (-) 351 Protein_1616 sigma-70 family RNA polymerase sigma factor -
  PF977_RS08235 (PF977_08235) nucA/comI 1587516..1587926 (+) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  PF977_RS08240 (PF977_08240) yqeB 1587959..1588681 (-) 723 WP_010886572.1 hypothetical protein -
  PF977_RS08245 (PF977_08245) gnd 1588933..1589826 (+) 894 WP_015714298.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  PF977_RS08250 (PF977_08250) yqeD 1589845..1590471 (-) 627 WP_015714297.1 TVP38/TMEM64 family protein -
  PF977_RS08255 (PF977_08255) cwlH 1590658..1591410 (+) 753 WP_015714296.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  PF977_RS08260 (PF977_08260) yqeF 1591663..1592394 (+) 732 WP_015714294.1 SGNH/GDSL hydrolase family protein -
  PF977_RS08265 (PF977_08265) - 1592700..1592840 (-) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=775098 PF977_RS08235 WP_009967785.1 1587516..1587926(+) (nucA/comI) [Bacillus subtilis strain SRCM124333]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=775098 PF977_RS08235 WP_009967785.1 1587516..1587926(+) (nucA/comI) [Bacillus subtilis strain SRCM124333]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACG
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTACCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529