Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   QU520_RS02655 Genome accession   NZ_CP128613
Coordinates   548843..550276 (-) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain WL297     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 543843..555276
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QU520_RS02620 (QU520_02620) elbB 544535..545188 (+) 654 WP_001352908.1 isoprenoid biosynthesis glyoxalase ElbB -
  QU520_RS02625 (QU520_02625) mtgA 545185..545913 (+) 729 WP_000047108.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  QU520_RS02630 (QU520_02630) yrbL 545910..546542 (-) 633 WP_000620388.1 PhoP regulatory network protein YrbL -
  QU520_RS02635 (QU520_02635) npr 546755..547027 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  QU520_RS02640 (QU520_02640) rapZ 547024..547878 (-) 855 WP_000243741.1 RNase adapter RapZ -
  QU520_RS02645 (QU520_02645) ptsN 547924..548415 (-) 492 WP_000183679.1 PTS IIA-like nitrogen regulatory protein PtsN -
  QU520_RS02650 (QU520_02650) hpf 548533..548820 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  QU520_RS02655 (QU520_02655) rpoN/rpoN1 548843..550276 (-) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  QU520_RS02660 (QU520_02660) lptB 550324..551049 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  QU520_RS02665 (QU520_02665) lptA 551056..551613 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  QU520_RS02670 (QU520_02670) lptC 551582..552157 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  QU520_RS02675 (QU520_02675) kdsC 552154..552720 (-) 567 WP_042003648.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  QU520_RS02680 (QU520_02680) kdsD 552741..553727 (-) 987 WP_001352909.1 arabinose-5-phosphate isomerase KdsD -
  QU520_RS02685 (QU520_02685) yrbG 553741..554718 (-) 978 WP_032185194.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=775078 QU520_RS02655 WP_000809051.1 548843..550276(-) (rpoN/rpoN1) [Escherichia coli strain WL297]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=775078 QU520_RS02655 WP_000809051.1 548843..550276(-) (rpoN/rpoN1) [Escherichia coli strain WL297]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCTATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCCTCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACAACGCAGACCTTGCAAGATTACCTGATGTGGCAAGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTTCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAAATCGACATCGATGAAGTTGAAGCCGTCCTGAAGCGGATCCAACGGTTTGATCC
GGTCGGCGTAGCGGCGAAAGATCTGCGTGACTGCCTGCTGATCCAACTCTCCCAATTCGATAAAACTACGCCGTGGCTGG
AAGAGGCGAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGTACCTTAATGCGCGTCACGCGA
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCACGCCCCGGACAGTCGATCCAGACTGG
TGAACCGGAGTATGTTATTCCAGATGTACTGGTGCGTAAGCATAACGGTCACTGGACAGTAGAACTCAACAGCGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGACGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTACGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGCGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCAACGGCAATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465