Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   PF976_RS05785 Genome accession   NZ_CP116011
Coordinates   1103360..1105189 (+) Length   609 a.a.
NCBI ID   WP_013351834.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain SRCM124317     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1098360..1110189
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PF976_RS05755 (PF976_05755) - 1099022..1099600 (+) 579 WP_013351830.1 GNAT family N-acetyltransferase -
  PF976_RS05760 (PF976_05760) spx 1099778..1100173 (+) 396 WP_003327578.1 transcriptional regulator Spx -
  PF976_RS05765 (PF976_05765) - 1100231..1100887 (-) 657 WP_013351831.1 TerC family protein -
  PF976_RS05775 (PF976_05775) mecA 1101163..1101819 (+) 657 WP_014470612.1 adaptor protein MecA Regulator
  PF976_RS05780 (PF976_05780) - 1101971..1103131 (+) 1161 WP_013351833.1 competence protein CoiA family protein -
  PF976_RS05785 (PF976_05785) pepF 1103360..1105189 (+) 1830 WP_013351834.1 oligoendopeptidase F Regulator
  PF976_RS05790 (PF976_05790) - 1105225..1105392 (-) 168 WP_003155026.1 hypothetical protein -
  PF976_RS05795 (PF976_05795) spxH 1105677..1106579 (-) 903 WP_013351835.1 protease adaptor protein SpxH -
  PF976_RS05800 (PF976_05800) - 1106576..1106974 (-) 399 WP_013351836.1 thiol management oxidoreductase -
  PF976_RS05805 (PF976_05805) - 1107199..1107885 (-) 687 WP_013351837.1 lytic transglycosylase domain-containing protein -
  PF976_RS05810 (PF976_05810) - 1107890..1108462 (-) 573 WP_013351838.1 CYTH domain-containing protein -
  PF976_RS05815 (PF976_05815) - 1108587..1108952 (+) 366 WP_013351839.1 hypothetical protein -
  PF976_RS05820 (PF976_05820) yjbM 1108980..1109615 (+) 636 WP_013351840.1 GTP diphosphokinase -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 69744.39 Da        Isoelectric Point: 5.4370

>NTDB_id=775021 PF976_RS05785 WP_013351834.1 1103360..1105189(+) (pepF) [Bacillus amyloliquefaciens strain SRCM124317]
MTEENKAAQLPDRSEVKAEHTWRLEDIFPSDEAWNKEFQAVKELIPNLSSFKGTLAHSADNLYAALTAQDEVTSRLGKLY
AYAHMRSDQDTGNSFYQGLNDKAASLYSQAASATAYIEPEILAIQEDKLHQFILEKEELKVYSHAIEEITKQRPHVLSEK
EEALLAEASEALSSPSNTFSVLNNADITFPSIKDENGKETQITHGNFINFLESSDREVRKNAFKAVYKTYGQYKNTMATT
LSGTVKKDNFYARVKKYKSAREAALSNNNIPEEVYDNLIKTINKHLPLLHRYIDLRKKVLGLDEVHNYDLYTPLVKDSGM
KVTYEEAKDYMLKGLAPLGDEYASILKEGLENRWVDVYENKGKRSGAYSSGTYGTNPYILMNWHDTVNNLFTLVHEFGHS
VHSYYTRKYQPYPYGNYSIFVAEVASTTNEALLGEYMLNSLKDEKQRLYLLNHMLEGFRGTVFRQTMFAEFEHLIHTKAQ
EGEPLTPELLNKLYYDLNKKYFGDNMVIDEEISLEWSRIPHFYYNYYVYQYATGYSAAQALSSQILKEGKPAVERYTNFL
KAGSSEYPIDILKKAGVDMTSPEPIEAACKMFEEKLNEMEELLSKVNPS

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=775021 PF976_RS05785 WP_013351834.1 1103360..1105189(+) (pepF) [Bacillus amyloliquefaciens strain SRCM124317]
ATGACTGAGGAAAATAAAGCAGCACAGCTGCCTGACAGAAGTGAAGTAAAGGCAGAGCATACATGGAGACTAGAGGATAT
TTTTCCTAGTGATGAGGCCTGGAATAAAGAATTTCAAGCAGTTAAAGAATTAATTCCGAATTTATCTTCCTTTAAAGGCA
CGCTTGCTCATTCAGCCGATAATTTATACGCGGCGCTTACCGCACAGGATGAAGTGACGAGCCGTCTGGGAAAACTTTAT
GCATACGCGCATATGCGCTCAGATCAGGATACGGGAAATTCTTTCTACCAAGGACTGAACGACAAAGCCGCAAGTCTGTA
TTCTCAGGCCGCCAGTGCGACTGCTTACATTGAACCGGAAATTCTGGCGATTCAGGAGGACAAGCTGCATCAGTTTATTC
TGGAAAAAGAAGAGCTTAAGGTTTACTCCCATGCCATTGAAGAAATTACAAAACAGCGTCCGCACGTGCTGAGCGAAAAA
GAAGAGGCCCTGCTTGCGGAAGCGTCAGAAGCGCTGTCTTCGCCTTCCAATACATTCAGTGTTTTAAACAACGCCGATAT
TACGTTTCCGTCGATAAAAGATGAAAACGGCAAGGAAACTCAAATCACACACGGCAACTTTATTAATTTTCTGGAAAGCA
GCGATCGGGAAGTCCGTAAAAACGCCTTTAAAGCCGTATATAAAACATACGGACAGTATAAAAACACGATGGCGACTACA
CTCAGTGGCACTGTGAAAAAAGATAATTTCTACGCCCGGGTGAAAAAATACAAATCAGCACGGGAAGCGGCTTTATCCAA
TAACAACATCCCTGAAGAAGTTTATGATAACCTTATCAAAACGATCAATAAACATCTTCCGTTATTACACCGTTACATTG
ATCTGCGGAAAAAGGTGCTCGGCCTTGATGAAGTCCACAATTATGACCTGTACACACCGCTTGTAAAAGATTCGGGAATG
AAGGTCACATATGAGGAAGCAAAAGATTATATGCTCAAAGGGCTTGCTCCGTTAGGCGATGAATACGCGTCTATTTTAAA
AGAAGGACTTGAAAACCGCTGGGTCGATGTATACGAAAACAAAGGAAAACGCAGCGGTGCTTATTCGTCCGGTACATACG
GCACGAATCCGTACATCTTAATGAACTGGCACGACACTGTCAATAATCTGTTTACGCTCGTTCACGAGTTCGGCCATTCC
GTGCACAGCTACTATACGAGAAAATATCAGCCGTATCCGTACGGAAACTACAGCATTTTTGTCGCTGAAGTCGCTTCTAC
GACCAATGAAGCGCTGCTCGGTGAATATATGCTGAACAGCTTAAAAGATGAAAAACAGCGTCTGTATCTTCTCAACCACA
TGCTTGAAGGTTTCAGAGGCACAGTCTTCAGACAGACGATGTTCGCTGAATTTGAGCATCTGATTCATACGAAAGCGCAA
GAAGGCGAGCCGTTAACGCCTGAACTGCTGAACAAGCTTTATTATGATCTGAATAAAAAGTATTTCGGAGACAACATGGT
AATTGATGAAGAAATCAGCCTGGAATGGTCAAGAATTCCGCATTTCTATTACAATTATTATGTGTATCAATATGCGACAG
GCTACAGTGCGGCTCAAGCGCTGAGCAGCCAGATCTTAAAAGAAGGCAAGCCTGCGGTTGAGCGTTATACCAACTTCTTA
AAAGCCGGAAGCTCAGAATATCCGATCGACATCCTGAAAAAAGCGGGAGTCGATATGACTTCGCCTGAACCGATCGAAGC
TGCCTGCAAAATGTTTGAAGAGAAACTGAATGAAATGGAAGAACTCCTTTCGAAAGTGAATCCTTCCTAA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(211-590)

Oligopeptidase F (Peptidase_M3_N)

(120-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

50

97.537

0.488