Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   PF976_RS05385 Genome accession   NZ_CP116011
Coordinates   1023630..1027334 (+) Length   1234 a.a.
NCBI ID   WP_014471678.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain SRCM124317     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1018630..1032334
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PF976_RS05370 (PF976_05370) - 1018693..1019220 (-) 528 WP_013351758.1 ferritin-like domain-containing protein -
  PF976_RS05375 (PF976_05375) - 1019289..1020002 (-) 714 WP_013351759.1 DUF421 domain-containing protein -
  PF976_RS05380 (PF976_05380) addB 1020143..1023643 (+) 3501 WP_014471677.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  PF976_RS05385 (PF976_05385) addA 1023630..1027334 (+) 3705 WP_014471678.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  PF976_RS05390 (PF976_05390) sbcD 1027401..1028573 (+) 1173 WP_014471679.1 exonuclease subunit SbcD -
  PF976_RS05395 (PF976_05395) sbcC 1028570..1031962 (+) 3393 WP_147785851.1 exonuclease subunit SbcC -
  PF976_RS05400 (PF976_05400) - 1031978..1032277 (+) 300 WP_013351764.1 hypothetical protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141365.90 Da        Isoelectric Point: 5.1885

>NTDB_id=775015 PF976_RS05385 WP_014471678.1 1023630..1027334(+) (addA) [Bacillus amyloliquefaciens strain SRCM124317]
MQIPKPKDSIWTDDQWSAIVSSGRDILVAAAAGSGKTAVLVERMIRKITAEEEPVDVDRLLVVTFTNASAAEMKHRIAEA
LEKELAKNPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYMIDLDPGFRMADQTEGELLGDEVLDELFEDEYAKGNQA
FYELVDRYTTDRHDLDLQDLVKRVYEYSRSHPDPEAWLKSFVRLYDVTEESKMEELPFYEYVREDAEMALFGAKQKLEKA
LELTKAPGGPAPRADNILDDLQQIEELISCRHDFKALYERVPAVTFKRAKAVKGDEFDKVLLDEATDLRNGAKKLIEKLK
TDYFTRSPQDHLKSLKDMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLAILTARDEEGRRVPSEAAVYYQDQ
FHEVLVDEYQDTNLVQESILQLVKSGNEESGNLFMVGDVKQSIYRFRLAEPLLFLGKYKRFTEDGEGAGQKIDLNQNFRS
RSDILDSTNFLFKQLMGGKIGEVDYDEQAALKLGASYPPNDAAKTELLLIDSADDADSPEDAEDFETVHWEAKAIAAEIR
KLVSSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQLMEELKNQGIPVYANLTSGYFEAVEVAAALSVLKVIDNPYQDI
PLASVLRSPIVGCDENELALIRLEKKKAPFYEALKAYIANGDRHDDLYQKLRIFYDSLQKWRSFSTNHSVSELIWEVYRD
TGYFDYAGGMPGGKQRQANLRVLYDRARSYEATAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFTAGLGRSFNMMDLNKSYLLDKELGFGTKYIHPELRISYPTLPLVAMKKKMRRELLSEELRVLYVALTRAKEKLFL
VGSCKNREKQLAKWQAQADRADWLLSEFDRYQASSYLDFIGPALIRHSDMEAHRAPGLLAPEDIAHDPARFHIRMLQQSE
LLEDSPEERAEEKSKRLKAIQHGEPIPDSFSFDDQARRLLEWEYPYRKLTAIRTKQSVSELKRKQEYEDEYSGRSLIKPS
GDTLLYKRPGFMMKKGLTAAEKGTAMHTVMQHIPLTHVPTAEEAERTVRMLYEKELLTEEQQDAIDIDEVIQFFGTEIGK
DLVGALRIDREVPFSMALPASEVYKDAETAGEPLLVQGIIDCLYETADGLYLLDYKTDRIEGKFRNGFEGAAPILQKRYE
TQIELYTKAVEQITKTNVKRRALYFFDGGHVLTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=775015 PF976_RS05385 WP_014471678.1 1023630..1027334(+) (addA) [Bacillus amyloliquefaciens strain SRCM124317]
ATGCAAATTCCTAAACCGAAAGACAGCATATGGACGGATGACCAATGGAGCGCCATCGTTTCTTCCGGCCGCGATATTCT
GGTCGCTGCCGCAGCCGGATCAGGTAAAACGGCCGTGCTTGTAGAGCGGATGATCAGAAAGATAACCGCGGAAGAAGAGC
CGGTTGATGTTGACAGGCTCCTTGTGGTGACATTTACAAACGCCTCAGCAGCGGAAATGAAGCATCGGATCGCTGAAGCA
TTAGAAAAAGAACTCGCCAAAAATCCGGGGTCTTTGCATATCAGACGGCAGCTGTCTCTATTAAATCGGGCGAGCATTTC
GACGCTGCACTCTTTCTGTCTGCAGGTGCTTAAAAAATATTATTATATGATTGATCTTGACCCGGGTTTTCGGATGGCTG
ACCAAACGGAAGGCGAATTGCTCGGAGACGAAGTGCTTGATGAGCTGTTTGAGGATGAATACGCAAAAGGGAATCAGGCG
TTTTATGAGCTTGTGGACAGATATACGACGGACAGGCATGATCTGGATCTTCAAGACCTCGTCAAGCGGGTGTATGAGTA
TTCCCGGTCGCATCCTGACCCTGAAGCGTGGCTGAAAAGCTTTGTCCGCCTATATGATGTGACAGAAGAAAGCAAAATGG
AAGAGCTGCCGTTTTACGAGTATGTGAGAGAAGATGCTGAAATGGCGCTCTTCGGGGCAAAACAAAAGCTAGAGAAGGCG
CTTGAACTGACAAAAGCGCCCGGCGGGCCGGCGCCGCGTGCTGATAATATTTTGGATGACCTTCAGCAGATAGAAGAGCT
GATCAGCTGCCGGCATGACTTTAAGGCGCTGTATGAACGGGTTCCTGCGGTCACATTTAAACGGGCCAAGGCGGTAAAGG
GTGATGAATTTGACAAGGTGCTTTTGGACGAAGCGACTGATTTGCGAAACGGCGCGAAGAAACTGATCGAAAAGTTAAAA
ACCGATTACTTTACAAGAAGCCCGCAAGATCATTTAAAGAGTTTAAAAGACATGAAGCCTGTTATTGAAACCCTCGTTCA
GCTCGTAATATCGTACGGAAAACGATTTGAAGCGGCGAAACAGGAAAAATCAATCATTGATTTCTCAGATTTGGAACATT
ACTGTCTGGCGATTTTAACTGCAAGGGATGAAGAAGGCCGGCGTGTGCCGAGTGAAGCGGCCGTTTATTATCAAGATCAG
TTCCATGAAGTTCTGGTTGATGAATATCAAGATACCAATCTTGTGCAGGAATCCATTTTGCAGCTTGTTAAAAGCGGGAA
TGAAGAGTCGGGAAATCTGTTTATGGTGGGGGACGTCAAGCAATCAATCTACCGTTTCAGACTTGCCGAGCCGCTGCTTT
TTCTCGGGAAATACAAAAGGTTTACGGAAGATGGGGAAGGCGCCGGGCAGAAAATCGACCTGAACCAAAACTTCCGAAGC
CGCTCCGATATTTTGGACAGTACGAATTTCTTGTTTAAGCAGCTGATGGGCGGCAAAATCGGCGAAGTGGACTACGATGA
ACAGGCGGCATTAAAGCTTGGCGCATCGTATCCGCCAAATGACGCGGCGAAAACGGAACTGCTGCTGATTGACAGCGCTG
ATGACGCAGACAGCCCCGAGGATGCTGAAGACTTTGAAACCGTGCATTGGGAAGCGAAAGCGATAGCCGCGGAGATCCGG
AAGCTTGTCTCATCTCCGTTTAAGGTATATGACGGCAAAACAAAAACACACCGGAATATCCAGTACCGGGATATCGTGAT
TTTGCTGCGTTCCATGCCATGGGCTCCGCAATTAATGGAAGAGCTGAAAAACCAGGGCATTCCGGTTTACGCCAATTTGA
CATCAGGTTACTTTGAAGCCGTTGAGGTGGCTGCGGCGCTTTCAGTCTTAAAGGTGATTGATAATCCGTATCAGGATATT
CCGCTTGCTTCCGTGCTGCGCTCGCCTATCGTCGGTTGTGACGAAAATGAACTCGCCCTCATACGCCTTGAAAAGAAAAA
AGCGCCGTTTTATGAAGCGCTGAAAGCGTATATAGCGAACGGGGACAGACATGACGACCTGTATCAAAAACTGAGAATAT
TTTATGACAGTCTGCAAAAGTGGCGGTCATTCTCGACGAATCATTCAGTGTCTGAGCTGATTTGGGAAGTATACCGCGAC
ACCGGATATTTTGATTATGCAGGCGGCATGCCTGGCGGAAAGCAGCGTCAGGCCAATCTGCGCGTCTTGTATGACCGGGC
GCGTTCGTATGAAGCGACGGCATTCCGCGGGTTATTCCGCTTTTTGCGGTTCATTGAACGGATGCAGGAGCGCGGCGATG
ATTTGGGGACGGCGCGGGCGCTCAGCGAGCAGGAGGATGTCGTTCGGCTGATGACGATCCACAGCAGCAAAGGACTTGAA
TTCCCCGTCGTTTTCACCGCCGGACTCGGCAGAAGTTTTAATATGATGGATCTCAACAAATCATACCTGCTTGATAAAGA
ACTCGGTTTCGGCACGAAGTATATTCATCCGGAACTTAGAATCAGCTATCCGACTCTGCCGCTTGTGGCAATGAAGAAAA
AAATGCGCAGAGAGCTTTTATCAGAAGAATTGCGCGTATTGTATGTGGCGCTGACAAGGGCGAAGGAAAAGCTGTTTCTC
GTCGGTTCCTGCAAAAACCGGGAAAAACAGCTGGCCAAATGGCAGGCGCAAGCAGACCGGGCTGATTGGCTCCTTTCTGA
ATTCGACCGCTATCAGGCGTCATCTTATTTGGACTTTATCGGTCCAGCGCTCATCCGTCACAGTGACATGGAGGCGCACA
GGGCGCCGGGGCTTTTGGCGCCGGAGGATATCGCGCACGACCCGGCGCGTTTTCACATCAGGATGCTGCAGCAAAGTGAA
TTGCTGGAAGATAGTCCTGAAGAACGTGCTGAAGAAAAGAGCAAACGTTTAAAAGCGATACAGCATGGCGAGCCGATTCC
TGATTCGTTTTCATTTGATGATCAGGCGCGCCGTCTTCTTGAATGGGAATATCCGTATCGGAAGCTGACCGCCATCAGAA
CGAAGCAGTCTGTTTCTGAGCTGAAGAGAAAGCAAGAATACGAGGATGAGTACAGCGGACGCTCTCTCATCAAACCGTCA
GGGGACACTCTTTTATACAAGCGTCCCGGCTTTATGATGAAAAAAGGGCTGACAGCCGCCGAAAAAGGGACGGCGATGCA
TACGGTGATGCAGCACATACCGCTGACGCATGTTCCGACTGCCGAAGAAGCGGAGCGGACGGTCAGGATGCTTTACGAAA
AAGAGCTGCTGACAGAAGAACAGCAGGACGCAATTGATATTGATGAAGTGATTCAGTTTTTCGGCACTGAAATCGGAAAA
GACCTTGTTGGGGCGCTGCGGATTGATCGGGAGGTGCCGTTCAGTATGGCGCTTCCGGCCAGCGAGGTGTACAAGGATGC
CGAAACGGCCGGTGAACCGCTGCTCGTCCAGGGAATTATTGACTGCCTCTATGAAACTGCGGACGGACTCTATTTGCTGG
ACTATAAAACGGATCGAATTGAGGGGAAATTCCGGAACGGATTTGAAGGCGCGGCTCCGATCCTGCAAAAAAGGTATGAA
ACACAAATCGAATTGTATACAAAAGCGGTTGAACAAATTACAAAAACGAATGTGAAAAGGCGTGCGCTCTATTTCTTCGA
TGGAGGTCACGTTCTTACACTGTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1226)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-881)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

81.848

100

0.818