Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   H7R74_RS01700 Genome accession   NZ_AP022281
Coordinates   347465..348904 (+) Length   479 a.a.
NCBI ID   WP_005336331.1    Uniprot ID   A0A0T6N7F3
Organism   Aeromonas veronii strain WP8-W19-CRE-03     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 342465..353904
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7R74_RS01670 (WP8W19C03_03160) mlaF 343042..343842 (-) 801 WP_005336343.1 phospholipid ABC transporter ATP-binding protein MlaF -
  H7R74_RS01675 (WP8W19C03_03170) - 344050..345045 (+) 996 WP_123172219.1 KpsF/GutQ family sugar-phosphate isomerase -
  H7R74_RS01680 (WP8W19C03_03180) kdsC 345045..345599 (+) 555 WP_182963125.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  H7R74_RS01685 (WP8W19C03_03190) lptC 345596..346156 (+) 561 WP_139460901.1 LPS export ABC transporter periplasmic protein LptC -
  H7R74_RS01690 (WP8W19C03_03200) lptA 346143..346670 (+) 528 WP_040068817.1 lipopolysaccharide transport periplasmic protein LptA -
  H7R74_RS01695 (WP8W19C03_03210) lptB 346674..347399 (+) 726 WP_005336333.1 LPS export ABC transporter ATP-binding protein -
  H7R74_RS01700 (WP8W19C03_03220) rpoN/rpoN1 347465..348904 (+) 1440 WP_005336331.1 RNA polymerase factor sigma-54 Machinery gene
  H7R74_RS01705 (WP8W19C03_03230) hpf 348925..349212 (+) 288 WP_005336323.1 ribosome hibernation promoting factor -
  H7R74_RS01710 (WP8W19C03_03240) ptsN 349215..349661 (+) 447 WP_005336322.1 PTS IIA-like nitrogen regulatory protein PtsN -
  H7R74_RS01715 (WP8W19C03_03250) rapZ 349707..350573 (+) 867 WP_182963126.1 RNase adapter RapZ -
  H7R74_RS01720 (WP8W19C03_03260) npr 350594..350866 (+) 273 WP_005336318.1 PTS phosphocarrier protein NPr -
  H7R74_RS01725 - 350884..352789 (-) 1906 Protein_330 BglG family transcription antiterminator -
  H7R74_RS01730 (WP8W19C03_03290) dagF 352897..353637 (-) 741 WP_182963127.1 2-dehydro-3-deoxy-phosphogluconate aldolase -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53701.64 Da        Isoelectric Point: 4.3442

>NTDB_id=77500 H7R74_RS01700 WP_005336331.1 347465..348904(+) (rpoN/rpoN1) [Aeromonas veronii strain WP8-W19-CRE-03]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALENNPLLEQEESEAQETSPEAPVDASQLDSSDAMAQEQMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTVALED
IQAAVTSDDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLDQYDPQTPWLNEAKEVILEHMELLGNRDYRTLARK
TKLKESDLKEVLDLIQLLEPRPGNGIIQSDSQYVIPDVSVVKKGSKWVVELNPDSAPRIRVNETYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLFIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=77500 H7R74_RS01700 WP_005336331.1 347465..348904(+) (rpoN/rpoN1) [Aeromonas veronii strain WP8-W19-CRE-03]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACTCTGGAACTTCAGCAGGAAATTCAGCAAGCGCTCGAAAACAACCCTCTGCTGGAGCAGGAAGAGAGCGAGG
CGCAAGAGACCAGTCCGGAAGCTCCGGTCGATGCCAGCCAGCTGGATTCCAGCGATGCCATGGCCCAGGAGCAGATGCCG
GATGAGTTGCCGGTGGATACCACCTGGGACGAGGTTTACTCTGCCGGCACCGCCCAGAGCGCGGGCCCCATCCACGATGG
GGAAGATAGCGTCTATCAGGGCGAAACCACCGAAAACCTGCAGGATTATCTGCTGTGGCAGATGCGGCTCACACCGTTCA
GCGATGTGGATGCCGCCATCGCGCTCGCCATCATTGATGCCATCGACGAATCCGGTTATCTGACCGTTGCGCTGGAGGAT
ATTCAGGCTGCCGTCACCAGCGATGACGTAGAGGTGGAACTCGATGAGGTCGAGGCGGTACTCAAGCGGGTGCAGCACTT
CGATCCGGTCGGCATTGCTGCACGTTCTGTGCAGGAGTGTTTGCTGATCCAGCTGGATCAGTATGACCCGCAGACCCCCT
GGCTCAACGAGGCCAAGGAGGTGATCCTCGAGCACATGGAGCTGCTCGGCAACCGGGATTACCGCACCCTCGCCCGCAAG
ACAAAACTCAAAGAGAGCGACCTCAAGGAGGTGCTGGATCTGATCCAGCTGCTGGAGCCGCGTCCCGGTAACGGCATCAT
CCAGAGCGACTCCCAGTACGTGATCCCCGACGTCTCCGTGGTCAAGAAGGGCAGCAAGTGGGTGGTCGAGCTCAATCCCG
ACTCCGCCCCCCGCATTCGGGTCAACGAGACCTATGCCGCCATGGCCCGCAACGCCCGCAGCAGCACCGACACCCAGTTT
ATCCGCTCCCATTTGCAGGAAGCCAAGTGGTTCATCAAGAGCCTGGAAAGTCGCAATGACACCCTGCTGAAAGTGGCCAG
TTGCATTGTCGAGCAGCAACAGGGCTTTTTCGAGTATGGTGAAGAGGCGATGAAGCCGATGGTGCTCAATGACATCGCCG
AAGCGGTAGAGATGCATGAGTCGACCATCTCCCGGGTGACGACCCAGAAGTACATGCATACCCCCCGTGGTATCTTCGAA
TTGAAGTTTTTTTTCTCCAGCCATGTGAACACCGAAGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTCATCAA
GAAGCTGGTCACGGCAGAGAATCCTGCCAAGCCGTTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCTAAGTATCGCGAATCCTTGTTTATCCCACCTTCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0T6N7F3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.988

100

0.491

Multiple sequence alignment