Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   PF975_RS18085 Genome accession   NZ_CP116010
Coordinates   3696229..3696666 (+) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain SRCM123815     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3691229..3701666
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PF975_RS18070 (PF975_18070) - 3694115..3694477 (-) 363 WP_007409352.1 winged helix-turn-helix transcriptional regulator -
  PF975_RS18075 (PF975_18075) hxlA 3694709..3695344 (+) 636 WP_014416875.1 3-hexulose-6-phosphate synthase -
  PF975_RS18080 (PF975_18080) hxlB 3695341..3695898 (+) 558 WP_014416874.1 6-phospho-3-hexuloisomerase -
  PF975_RS18085 (PF975_18085) nucA/comI 3696229..3696666 (+) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  PF975_RS18090 (PF975_18090) nin/comJ 3696687..3697085 (+) 399 WP_014416873.1 competence protein ComJ Regulator
  PF975_RS18095 (PF975_18095) - 3697126..3698562 (-) 1437 WP_014416872.1 glycoside hydrolase family 1 protein -
  PF975_RS18100 (PF975_18100) - 3698666..3698944 (-) 279 WP_263612330.1 YckD family protein -
  PF975_RS18105 (PF975_18105) - 3699061..3699495 (-) 435 WP_014416871.1 RDD family protein -
  PF975_RS18110 (PF975_18110) - 3699510..3700646 (-) 1137 WP_007609290.1 zinc-dependent alcohol dehydrogenase -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=774992 PF975_RS18085 WP_003156588.1 3696229..3696666(+) (nucA/comI) [Bacillus velezensis strain SRCM123815]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=774992 PF975_RS18085 WP_003156588.1 3696229..3696666(+) (nucA/comI) [Bacillus velezensis strain SRCM123815]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAGGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641