Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PE062_RS24510 Genome accession   NZ_CP116006
Coordinates   5319324..5320769 (+) Length   481 a.a.
NCBI ID   WP_271263949.1    Uniprot ID   -
Organism   Achromobacter mucicolens strain DMF-4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5314324..5325769
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PE062_RS24495 (PE062_24495) - 5314921..5316249 (+) 1329 WP_271263948.1 amidase -
  PE062_RS24500 (PE062_24500) leuA 5316434..5318144 (-) 1711 Protein_4849 2-isopropylmalate synthase -
  PE062_RS24505 (PE062_24505) - 5318663..5319325 (-) 663 WP_128829726.1 hypothetical protein -
  PE062_RS24510 (PE062_24510) rpoN/rpoN1 5319324..5320769 (+) 1446 WP_271263949.1 RNA polymerase factor sigma-54 Machinery gene
  PE062_RS24515 (PE062_24515) - 5320891..5321169 (+) 279 WP_056327752.1 (2Fe-2S)-binding protein -
  PE062_RS24520 (PE062_24520) - 5321199..5322062 (-) 864 WP_271263950.1 LysR substrate-binding domain-containing protein -
  PE062_RS24525 (PE062_24525) - 5322196..5323107 (+) 912 WP_271263951.1 DMT family transporter -
  PE062_RS24530 (PE062_24530) bfr 5323485..5323961 (+) 477 WP_056327755.1 bacterioferritin -
  PE062_RS24535 (PE062_24535) - 5324055..5324729 (-) 675 WP_271263952.1 hypothetical protein -

Sequence

Protein


Download         Length: 481 a.a.        Molecular weight: 52603.90 Da        Isoelectric Point: 4.7628

>NTDB_id=774869 PE062_RS24510 WP_271263949.1 5319324..5320769(+) (rpoN/rpoN1) [Achromobacter mucicolens strain DMF-4]
MQNIVPMTRPILELRPGQHLTLTPQLQQSIRLLQLSTLDLEAEISQVLAENPLLDREDEPATAEDRADSERESSVQDDEP
AAERETAIDEMPGSGGVYPDDDASLPEAARPDTLREHLLGQLALTRAAPRDAALASLLIDELDENGYLGSPLDEILGWLP
AEMEPDMDELRAALSLLQSFDPVGIGARDMAECLQLQLRNPDLTLLPEAADPAVLACARRICAEHLPLLATGNAARLCAA
VGCDETTFRAAHSLILRLEPRPGRAWTVPAAEYAVPDVIVRKTRRGWQATLNSAAVPRLQINGLYAQMLGNQKESAHAGL
QAQLQQAKWMIRNVEQRFDTILRVSQAIVERQTAFFSQGPAAMRPLILKDIAGELGLHESTISRATTQKYMLTPFGTLEL
KRFFGTGVSTEGGDATSATAVQTYIRQMVADENRAKPLSDSQIMQKLADQGIVIARRTVAKYREALRIAPAALRKAQASA
R

Nucleotide


Download         Length: 1446 bp        

>NTDB_id=774869 PE062_RS24510 WP_271263949.1 5319324..5320769(+) (rpoN/rpoN1) [Achromobacter mucicolens strain DMF-4]
ATGCAAAATATTGTACCTATGACCCGTCCAATTTTAGAACTGCGGCCTGGCCAGCACCTGACGCTGACCCCACAACTTCA
GCAATCGATCCGATTGTTGCAGTTGTCCACGCTCGACCTTGAAGCGGAAATCTCGCAAGTGCTGGCCGAGAATCCCCTGC
TGGATCGCGAGGACGAGCCGGCGACGGCGGAGGATCGGGCCGATTCCGAGCGCGAATCTTCGGTTCAGGACGACGAACCG
GCGGCCGAGCGCGAGACTGCCATCGACGAGATGCCCGGCTCGGGCGGGGTGTATCCCGACGACGACGCCAGCCTGCCCGA
GGCGGCGCGGCCGGACACGCTGCGCGAACATCTGCTGGGTCAGCTTGCCCTGACGCGCGCCGCGCCGCGCGATGCCGCGC
TGGCGTCCCTGCTGATCGACGAACTGGACGAAAACGGCTACCTGGGTTCGCCGCTGGATGAAATCCTGGGTTGGCTCCCT
GCCGAAATGGAGCCCGACATGGACGAGCTGCGCGCCGCCCTGTCGCTTCTGCAATCGTTCGACCCGGTGGGCATCGGCGC
GCGCGACATGGCCGAATGCCTGCAACTGCAGCTGCGCAATCCCGACCTCACCTTGCTGCCCGAGGCGGCCGACCCCGCCG
TGCTGGCCTGTGCGCGCCGGATCTGCGCCGAGCACCTGCCGCTGTTGGCAACCGGCAACGCCGCGCGCCTGTGTGCGGCG
GTCGGCTGCGACGAGACCACGTTCCGCGCGGCGCATTCGCTGATCCTGCGCCTGGAACCCCGGCCGGGCCGGGCCTGGAC
CGTGCCGGCGGCCGAGTACGCAGTGCCGGATGTCATCGTCCGCAAGACGCGGCGCGGCTGGCAGGCCACGCTGAATAGCG
CGGCGGTGCCGCGGCTGCAGATCAATGGGCTGTACGCCCAGATGCTGGGCAACCAGAAGGAATCCGCCCACGCCGGCCTG
CAGGCCCAGCTGCAGCAGGCCAAATGGATGATCCGCAACGTGGAGCAGCGCTTTGACACCATCCTGCGCGTGTCGCAGGC
CATCGTCGAGCGGCAGACGGCTTTCTTCAGCCAGGGGCCGGCGGCCATGCGTCCACTCATCCTGAAGGACATCGCCGGCG
AGCTGGGCTTGCATGAATCGACCATTTCACGGGCCACCACACAGAAGTACATGCTCACTCCATTCGGTACGCTGGAGCTC
AAGCGCTTCTTTGGAACGGGCGTGTCGACAGAAGGCGGCGACGCCACGTCGGCCACCGCGGTGCAGACCTACATCCGCCA
GATGGTGGCCGACGAGAACCGCGCCAAGCCATTGTCGGATAGTCAGATCATGCAAAAACTGGCCGATCAGGGGATAGTGA
TTGCGCGCCGGACGGTGGCAAAGTACCGCGAAGCGCTGCGGATTGCCCCTGCCGCGCTGCGCAAGGCGCAGGCCTCCGCA
CGCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(108-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(320-475)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(12-55)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

49.105

100

0.514