Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   QUR99_RS02740 Genome accession   NZ_CP128594
Coordinates   461291..461968 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain VG1046-3E9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 456291..466968
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUR99_RS02725 (QUR99_02725) chiA 457658..460351 (+) 2694 WP_020239449.1 bifunctional chitinase/lysozyme -
  QUR99_RS02730 (QUR99_02730) bfd 460520..460714 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  QUR99_RS02735 (QUR99_02735) bfr 460786..461262 (+) 477 WP_000675504.1 bacterioferritin -
  QUR99_RS02740 (QUR99_02740) pilD 461291..461968 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  QUR99_RS02745 (QUR99_02745) gspM 461968..462429 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  QUR99_RS02750 (QUR99_02750) gspL 462426..463589 (-) 1164 WP_149857382.1 type II secretion system protein GspL -
  QUR99_RS02755 (QUR99_02755) gspK 463604..464587 (-) 984 WP_021570243.1 type II secretion system minor pseudopilin GspK -
  QUR99_RS02760 (QUR99_02760) gspJ 464580..465167 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  QUR99_RS02765 (QUR99_02765) gspI 465160..465537 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  QUR99_RS02770 (QUR99_02770) gspH 465534..466043 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  QUR99_RS02775 (QUR99_02775) gspG 466051..466488 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=774829 QUR99_RS02740 WP_000178161.1 461291..461968(-) (pilD) [Escherichia coli strain VG1046-3E9]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=774829 QUR99_RS02740 WP_000178161.1 461291..461968(-) (pilD) [Escherichia coli strain VG1046-3E9]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378