Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   QUS01_RS03395 Genome accession   NZ_CP128590
Coordinates   524560..525993 (-) Length   477 a.a.
NCBI ID   WP_000809036.1    Uniprot ID   -
Organism   Escherichia coli strain VG35A2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 519560..530993
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUS01_RS03360 (QUS01_03360) elbB 520251..520904 (+) 654 WP_001297560.1 isoprenoid biosynthesis glyoxalase ElbB -
  QUS01_RS03365 (QUS01_03365) mtgA 520901..521629 (+) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  QUS01_RS03370 (QUS01_03370) yrbL 521626..522258 (-) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  QUS01_RS03375 (QUS01_03375) npr 522472..522744 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  QUS01_RS03380 (QUS01_03380) rapZ 522741..523595 (-) 855 WP_000243741.1 RNase adapter RapZ -
  QUS01_RS03385 (QUS01_03385) ptsN 523641..524132 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  QUS01_RS03390 (QUS01_03390) hpf 524250..524537 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  QUS01_RS03395 (QUS01_03395) rpoN/rpoN1 524560..525993 (-) 1434 WP_000809036.1 RNA polymerase factor sigma-54 Machinery gene
  QUS01_RS03400 (QUS01_03400) lptB 526041..526766 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  QUS01_RS03405 (QUS01_03405) lptA 526773..527330 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  QUS01_RS03410 (QUS01_03410) lptC 527299..527874 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  QUS01_RS03415 (QUS01_03415) kdsC 527871..528437 (-) 567 WP_000030005.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  QUS01_RS03420 (QUS01_03420) kdsD 528458..529444 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  QUS01_RS03425 (QUS01_03425) yrbG 529458..530435 (-) 978 WP_137588146.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54007.82 Da        Isoelectric Point: 4.3639

>NTDB_id=774671 QUS01_RS03395 WP_000809036.1 524560..525993(-) (rpoN/rpoN1) [Escherichia coli strain VG35A2]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPIYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=774671 QUS01_RS03395 WP_000809036.1 524560..525993(-) (rpoN/rpoN1) [Escherichia coli strain VG35A2]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
TGAGCTGCCGATCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAGGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTAGCGGCAAAAGATCTGCGTGACTGCCTGCTAATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465