Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   QUS02_RS21325 Genome accession   NZ_CP128584
Coordinates   4142717..4143394 (+) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain VG48B2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4137717..4148394
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUS02_RS21290 (QUS02_21290) gspG 4138197..4138634 (+) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  QUS02_RS21295 (QUS02_21295) gspH 4138642..4139151 (+) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  QUS02_RS21300 (QUS02_21300) gspI 4139148..4139525 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  QUS02_RS21305 (QUS02_21305) gspJ 4139518..4140105 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  QUS02_RS21310 (QUS02_21310) gspK 4140098..4141081 (+) 984 WP_047612831.1 type II secretion system minor pseudopilin GspK -
  QUS02_RS21315 (QUS02_21315) gspL 4141096..4142259 (+) 1164 WP_001115238.1 type II secretion system protein GspL -
  QUS02_RS21320 (QUS02_21320) gspM 4142256..4142717 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  QUS02_RS21325 (QUS02_21325) pilD 4142717..4143394 (+) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  QUS02_RS21330 (QUS02_21330) bfr 4143423..4143899 (-) 477 WP_000675504.1 bacterioferritin -
  QUS02_RS21335 (QUS02_21335) bfd 4143971..4144165 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  QUS02_RS21340 (QUS02_21340) chiA 4144334..4147027 (-) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=774640 QUS02_RS21325 WP_000178161.1 4142717..4143394(+) (pilD) [Escherichia coli strain VG48B2]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=774640 QUS02_RS21325 WP_000178161.1 4142717..4143394(+) (pilD) [Escherichia coli strain VG48B2]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378