Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PG907_RS02235 Genome accession   NZ_CP115955
Coordinates   476651..478150 (-) Length   499 a.a.
NCBI ID   WP_193026583.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain RUN2340     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 471651..483150
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PG907_RS02205 - 472161..472811 (-) 651 WP_193026586.1 LON peptidase substrate-binding domain-containing protein -
  PG907_RS02210 rapZ 472889..473779 (-) 891 WP_193026585.1 RNase adapter RapZ -
  PG907_RS02215 - 473873..474208 (-) 336 WP_193028556.1 PsiF family protein -
  PG907_RS02220 hprK 474397..475371 (-) 975 WP_011000367.1 HPr(Ser) kinase/phosphatase -
  PG907_RS02225 ptsN 475442..475897 (-) 456 WP_003269869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  PG907_RS02230 hpf 476246..476599 (-) 354 WP_193026584.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  PG907_RS02235 rpoN/rpoN1 476651..478150 (-) 1500 WP_193026583.1 RNA polymerase factor sigma-54 Machinery gene
  PG907_RS02240 lptB 478248..479036 (-) 789 WP_193026582.1 LPS export ABC transporter ATP-binding protein -
  PG907_RS02245 lptA 479070..479645 (-) 576 WP_193026581.1 lipopolysaccharide transport periplasmic protein LptA -
  PG907_RS02250 lptC 479715..480347 (-) 633 WP_193026580.1 LPS export ABC transporter periplasmic protein LptC -
  PG907_RS02255 - 480349..480936 (-) 588 WP_193026579.1 KdsC family phosphatase -
  PG907_RS02260 - 480968..481951 (-) 984 WP_193026578.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55184.55 Da        Isoelectric Point: 4.8441

>NTDB_id=774594 PG907_RS02235 WP_193026583.1 476651..478150(-) (rpoN/rpoN1) [Ralstonia pseudosolanacearum strain RUN2340]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGADGSVSALQSSSATPMPTE
SPPPTANGADHPDTAEASYENGSDFDSDYGTDRSDWSLDDFARKPQSDEDDRAPMQLRDAEPTQREFLMEQLAPLKLSPR
DKGLVIFLIESLDDEGYLTGSLDEILADLPPELEVELDELQAALRMLQSFDPAGIGARSAAECLTLQLQRLDSPAKPLAL
AIVNQHLELLAARDYTRLKKALSVDDGALKAAHELIRSLAPFPGHAFGRAEADFVVPDVVVRKTAAGWMAQLNPDVMPRL
RINDMYAQILRSSRGESGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTLGLH
ESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVARRT
VAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=774594 PG907_RS02235 WP_193026583.1 476651..478150(-) (rpoN/rpoN1) [Ralstonia pseudosolanacearum strain RUN2340]
ATGAAACAGTCGCTCCAGCTCCGCCTTTCCCAGCATCTGGCGCTCACGCCGCAACTGCAGCAGTCGATCCGGCTGCTGCA
GTTGTCGACCATCGAACTGCAACAGGAAGTCGAGCAGGCGCTGACTGAGAACCCGCTGCTTGAGCGCGAGAACGAGTGGC
TCGACACCTCCGCGCGCATCGGCGCCGACGGCTCGGTCAGCGCCCTGCAGAGCAGCAGTGCCACCCCGATGCCGACCGAA
TCGCCGCCGCCCACCGCCAACGGCGCGGACCATCCCGACACGGCCGAGGCATCGTACGAAAACGGCAGCGATTTCGACAG
CGACTACGGCACCGATCGGTCCGACTGGAGCCTCGACGACTTCGCGCGCAAACCCCAGTCCGACGAAGACGACCGCGCGC
CGATGCAGCTGCGCGATGCCGAACCCACGCAGCGCGAATTCCTGATGGAACAGCTCGCGCCGCTCAAGCTGTCCCCGCGC
GACAAGGGCCTCGTCATCTTCCTGATCGAATCGCTGGACGACGAAGGCTACCTGACCGGCTCGCTCGATGAGATCCTCGC
CGATCTGCCGCCCGAACTCGAGGTCGAGCTCGATGAATTGCAAGCCGCGCTGCGGATGCTGCAGAGCTTCGATCCGGCCG
GCATCGGTGCGCGCTCGGCGGCCGAATGCCTGACGCTGCAGCTGCAGCGCCTGGACTCCCCAGCCAAGCCGCTGGCGCTG
GCCATCGTCAACCAGCATCTCGAACTGCTCGCCGCGCGCGACTACACGCGGCTGAAGAAAGCGCTGTCCGTCGATGATGG
CGCGCTGAAGGCCGCGCATGAGCTGATCCGCTCGCTGGCGCCCTTCCCGGGCCACGCCTTCGGGCGGGCCGAGGCCGACT
TCGTCGTGCCCGACGTGGTGGTGCGCAAGACCGCCGCGGGCTGGATGGCACAGCTGAACCCCGACGTGATGCCACGCCTG
CGCATCAACGACATGTATGCGCAGATCCTGCGCAGCAGCCGTGGCGAATCGGGCGCCGCCAACCTGCAGCAGAAGCTGCA
AGAAGCGCGCTGGCTGATCAAAAATATCCAGCAGCGCTTCGACACCATCCTGCGTGTCTCGCAAGCCATTGTCGAACGTC
AAAAGAGCTTTTTCACGCACGGTGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACACTGGGTCTACAC
GAGTCCACGATCTCCCGTGTGACGACCAACAAATACATGGCAACCCCCATGGGGACCTTCGAGCTGAAGTACTTCTTCGG
CAGCCATGTATCGACGGAAACCGGCGGGGCCGCGTCATCCACCGCGATACGCGCGCTCATCAAGCAACTTGTAGGAGCCG
AAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTGGCCCGTCGGACG
GTGGCCAAGTACCGCGAAGCCCTCAAGATCCCAGCAGTGAATCTGCGCAAGTCTTTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-497)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

98.196

100

0.982