Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PG909_RS01885 Genome accession   NZ_CP115946
Coordinates   388165..389664 (-) Length   499 a.a.
NCBI ID   WP_003262276.1    Uniprot ID   A0AAE3NGW1
Organism   Ralstonia solanacearum MolK2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 383165..394664
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PG909_RS01855 - 383672..384325 (-) 654 WP_003278696.1 LON peptidase substrate-binding domain-containing protein -
  PG909_RS01860 rapZ 384403..385293 (-) 891 WP_014618030.1 RNase adapter RapZ -
  PG909_RS01865 - 385387..385719 (-) 333 WP_003278700.1 PsiF family protein -
  PG909_RS01870 hprK 385910..386884 (-) 975 WP_003262273.1 HPr(Ser) kinase/phosphatase -
  PG909_RS01875 ptsN 386955..387410 (-) 456 WP_003262274.1 PTS IIA-like nitrogen regulatory protein PtsN -
  PG909_RS01880 hpf 387760..388113 (-) 354 WP_003262275.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  PG909_RS01885 rpoN/rpoN1 388165..389664 (-) 1500 WP_003262276.1 RNA polymerase factor sigma-54 Machinery gene
  PG909_RS01890 lptB 389762..390550 (-) 789 WP_003262277.1 LPS export ABC transporter ATP-binding protein -
  PG909_RS01895 lptA 390584..391180 (-) 597 WP_003278705.1 lipopolysaccharide transport periplasmic protein LptA -
  PG909_RS01900 lptC 391248..391880 (-) 633 WP_003262280.1 LPS export ABC transporter periplasmic protein LptC -
  PG909_RS01905 - 391882..392469 (-) 588 WP_003278712.1 KdsC family phosphatase -
  PG909_RS01910 - 392502..393485 (-) 984 WP_014618022.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55158.49 Da        Isoelectric Point: 4.8522

>NTDB_id=774558 PG909_RS01885 WP_003262276.1 388165..389664(-) (rpoN/rpoN1) [Ralstonia solanacearum MolK2]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGADGSVSALQSNSATPPPTE
SPPPAANGSDHADTAEASYENGSDFDSDYGSDRSDWSLDDFARKPQSDEDDRAPMQLREAEPTLREFLMEQLAPLKLSTR
DKGLVIFLIESLDDEGYLTGSLDEILADLPAELEVELDELQAALRMLQSFDPAGIGARSAAECLTLQLQRLESPAKPLAL
AIVNQHLELLAARDYTRLKKALSVDESALKAAHELIRSLAPFPGHAFGRAEADFVVPDVVVRKTAAGWMAQLNPDVMPRL
RINDMYAQILRSSRGESGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTLGLH
ESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVARRT
VAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=774558 PG909_RS01885 WP_003262276.1 388165..389664(-) (rpoN/rpoN1) [Ralstonia solanacearum MolK2]
ATGAAACAGTCGCTCCAGCTCCGTCTCTCTCAGCATCTGGCGCTCACGCCGCAACTGCAGCAGTCGATCCGGCTGCTGCA
GTTGTCGACCATCGAACTGCAACAGGAAGTCGAGCAGGCGCTGACCGAGAACCCGCTGCTGGAGCGCGAGAACGAGTGGC
TCGACACCTCTGCGCGCATCGGTGCCGATGGTTCGGTCAGCGCGCTGCAGAGCAACAGCGCCACCCCGCCGCCGACCGAA
TCGCCGCCGCCCGCCGCCAACGGCTCGGACCACGCCGACACAGCCGAAGCATCGTACGAAAACGGCAGCGACTTCGACAG
CGACTACGGCTCCGATCGCTCCGACTGGAGCCTCGACGACTTCGCTCGCAAACCCCAGTCCGACGAAGACGACCGCGCCC
CGATGCAGCTGCGCGAGGCCGAGCCCACGCTGCGCGAATTCCTGATGGAACAGCTGGCGCCGCTCAAGCTGTCCACGCGC
GACAAGGGCCTCGTCATCTTCCTGATCGAGTCGCTGGACGACGAGGGCTACCTGACCGGCTCGCTCGACGAGATCCTCGC
CGACCTGCCGGCCGAACTCGAGGTCGAACTCGACGAACTGCAGGCCGCGCTGCGCATGCTGCAGAGCTTCGACCCGGCCG
GCATCGGTGCACGCTCGGCGGCGGAATGCCTGACGCTGCAGTTGCAGCGCCTGGAGTCGCCAGCCAAGCCGCTCGCGCTG
GCCATCGTCAACCAGCATCTCGAGCTGCTCGCCGCGCGCGATTACACGCGCCTGAAAAAAGCGCTGTCCGTCGACGAAAG
CGCGCTGAAGGCCGCGCATGAACTGATCCGCTCGCTGGCGCCGTTCCCGGGCCACGCCTTCGGGCGCGCCGAGGCCGACT
TCGTCGTGCCCGATGTCGTGGTGCGTAAAACCGCCGCAGGCTGGATGGCGCAACTGAATCCCGACGTGATGCCGCGCCTG
CGCATCAACGACATGTACGCGCAGATCCTGCGCAGCAGCCGCGGCGAGTCGGGCGCCGCGAACCTGCAGCAAAAGCTGCA
GGAAGCGCGCTGGCTGATCAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGTGTCTCGCAAGCGATTGTCGAACGTC
AAAAGAGCTTTTTCACGCACGGCGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACACTGGGTCTACAC
GAGTCCACGATCTCCCGTGTGACGACCAACAAGTACATGGCGACCCCCATGGGGACCTTCGAGCTGAAGTACTTCTTCGG
CAGCCATGTATCGACGGAAACCGGCGGGGCCGCGTCATCCACCGCGATACGCGCGCTCATCAAGCAACTTGTAGGAGCCG
AAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTGGCCCGTCGGACG
GTGGCCAAGTACCGCGAAGCCCTCAAAATCCCAGCAGTGAATTTGCGCAAGTCTTTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-497)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

97.395

100

0.974