Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PG906_RS02130 Genome accession   NZ_CP115944
Coordinates   441621..443120 (-) Length   499 a.a.
NCBI ID   WP_275794339.1    Uniprot ID   G3A498
Organism   Ralstonia syzygii R24     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 436621..448120
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PG906_RS02100 - 437134..437784 (-) 651 WP_134927698.1 LON peptidase substrate-binding domain-containing protein -
  PG906_RS02105 rapZ 437864..438754 (-) 891 WP_013213615.1 RNase adapter RapZ -
  PG906_RS02110 - 438847..439176 (-) 330 WP_197329701.1 PsiF family protein -
  PG906_RS02115 hprK 439368..440342 (-) 975 WP_197331673.1 HPr(Ser) kinase/phosphatase -
  PG906_RS02120 ptsN 440413..440868 (-) 456 WP_197331672.1 PTS IIA-like nitrogen regulatory protein PtsN -
  PG906_RS02125 hpf 441216..441569 (-) 354 WP_013213613.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  PG906_RS02130 rpoN/rpoN1 441621..443120 (-) 1500 WP_275794339.1 RNA polymerase factor sigma-54 Machinery gene
  PG906_RS02135 lptB 443218..444006 (-) 789 WP_197331671.1 LPS export ABC transporter ATP-binding protein -
  PG906_RS02140 lptA 444040..444621 (-) 582 WP_197331670.1 lipopolysaccharide transport periplasmic protein LptA -
  PG906_RS02145 lptC 444690..445322 (-) 633 WP_197331669.1 LPS export ABC transporter periplasmic protein LptC -
  PG906_RS02150 - 445324..445911 (-) 588 WP_013213608.1 KdsC family phosphatase -
  PG906_RS02155 - 445944..446927 (-) 984 WP_197329709.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55052.44 Da        Isoelectric Point: 4.8496

>NTDB_id=774537 PG906_RS02130 WP_275794339.1 441621..443120(-) (rpoN/rpoN1) [Ralstonia syzygii R24]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGVDGSVSALQSSSATPPPTE
SPPPAANGADHADTAETSYENGSDFDSDYGADRSDWSLDDFARKPQSDEDDRAPMQLREAEPTLREFLMEQLAPLKLSLR
DKGLVIFLIESLDDEGYLTGSLDEILADLPAELEAELDELQAALRMLQSFDPAGIGARSAAECLTLQLQRLDSPAKPLAL
AIVSQHLELLAARDYTRLKKALSVDEGALKAAHELIRSLAPFPGHAFGRAEADFVVPDVVVRKTAAGWMAQLNPDVMPRL
RINDMYAQILRSSRGESGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTLGLH
ESTISRVTTNKYMATPLGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVARRT
VAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=774537 PG906_RS02130 WP_275794339.1 441621..443120(-) (rpoN/rpoN1) [Ralstonia syzygii R24]
ATGAAACAGTCGCTCCAGCTCCGTCTCTCTCAGCATCTGGCGCTCACGCCGCAACTGCAGCAGTCGATCCGGCTGCTGCA
GCTGTCGACCATCGAGCTGCAACAGGAAGTCGAGCAAGCGCTGACTGAGAACCCGCTGCTTGAGCGCGAGAACGAGTGGC
TCGACACCTCCGCGCGCATCGGCGTCGACGGCTCGGTCAGTGCCCTGCAGAGCAGCAGCGCCACCCCGCCGCCGACCGAA
TCGCCGCCGCCCGCCGCCAACGGCGCGGACCACGCCGACACTGCCGAGACATCGTACGAAAACGGCAGCGATTTCGACAG
CGACTATGGCGCCGATCGTTCCGACTGGAGCCTCGACGACTTCGCACGCAAACCGCAGTCGGACGAAGACGACCGCGCCC
CGATGCAGCTGCGCGAGGCCGAACCCACGCTGCGCGAATTCCTGATGGAACAGCTGGCGCCGCTCAAGCTGTCCCTGCGC
GACAAAGGCCTCGTCATCTTCCTGATCGAGTCGCTGGACGACGAAGGCTACCTGACCGGCTCGCTCGATGAGATCCTCGC
CGACCTGCCGGCCGAACTCGAGGCCGAACTCGACGAACTGCAGGCCGCGCTGCGCATGCTGCAGAGCTTCGATCCGGCCG
GTATCGGCGCGCGCTCGGCGGCGGAATGCCTGACGTTGCAGCTGCAGCGCCTGGACTCGCCGGCCAAGCCGCTGGCGCTG
GCCATCGTCAGCCAGCATCTCGAACTGCTCGCCGCGCGGGACTACACACGCCTGAAGAAAGCGTTGTCCGTCGATGAAGG
CGCGCTGAAGGCCGCGCATGAACTGATCCGCTCGCTGGCCCCGTTCCCTGGCCACGCCTTCGGGCGCGCCGAGGCCGACT
TTGTCGTGCCCGACGTGGTGGTGCGTAAAACTGCCGCAGGCTGGATGGCACAGTTGAACCCCGACGTGATGCCGCGCCTG
CGCATCAACGACATGTACGCGCAGATCCTGCGCAGCAGCCGAGGCGAATCGGGCGCCGCCAACCTGCAGCAGAAGCTGCA
GGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGTGTCTCGCAAGCGATTGTCGAACGTC
AAAAGAGCTTTTTCACGCACGGCGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACACTGGGTCTACAC
GAGTCCACGATCTCCCGTGTGACGACCAACAAGTACATGGCGACCCCCCTGGGGACCTTCGAGCTGAAGTACTTCTTCGG
CAGCCATGTATCGACGGAAACCGGCGGGGCCGCGTCATCCACTGCGATACGCGCGCTCATCAAGCAACTTGTAGGAGCCG
AAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTGGCCCGTCGGACG
GTGGCCAAGTACCGCGAAGCCCTCAAAATCCCTGCAGTGAATTTGCGTAAGTCTTTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-497)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3A498

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

97.194

100

0.972