Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   QUS00_RS03340 Genome accession   NZ_CP128581
Coordinates   470364..471041 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain VG888-46E9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 465364..476041
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUS00_RS03325 (QUS00_03320) chiA 466731..469424 (+) 2694 WP_087934036.1 bifunctional chitinase/lysozyme -
  QUS00_RS03330 (QUS00_03325) bfd 469593..469787 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  QUS00_RS03335 (QUS00_03330) bfr 469859..470335 (+) 477 WP_000675504.1 bacterioferritin -
  QUS00_RS03340 (QUS00_03335) pilD 470364..471041 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  QUS00_RS03345 (QUS00_03340) gspM 471041..471502 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  QUS00_RS03350 (QUS00_03345) gspL 471499..472662 (-) 1164 WP_001115230.1 type II secretion system protein GspL -
  QUS00_RS03355 (QUS00_03350) gspK 472677..473660 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  QUS00_RS03360 (QUS00_03355) gspJ 473653..474240 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  QUS00_RS03365 (QUS00_03360) gspI 474233..474610 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  QUS00_RS03370 (QUS00_03365) gspH 474607..475116 (-) 510 WP_001076048.1 type II secretion system minor pseudopilin GspH -
  QUS00_RS03375 (QUS00_03370) gspG 475124..475561 (-) 438 WP_001202880.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=774514 QUS00_RS03340 WP_000178161.1 470364..471041(-) (pilD) [Escherichia coli strain VG888-46E9]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=774514 QUS00_RS03340 WP_000178161.1 470364..471041(-) (pilD) [Escherichia coli strain VG888-46E9]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378