Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   PGN40_RS17365 Genome accession   NZ_CP115914
Coordinates   3480804..3481418 (-) Length   204 a.a.
NCBI ID   WP_040056824.1    Uniprot ID   A0A0H4KPF0
Organism   Priestia filamentosa strain AZC66     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3475804..3486418
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PGN40_RS17340 (PGN40_17340) rpsT 3475951..3476217 (+) 267 WP_019391417.1 30S ribosomal protein S20 -
  PGN40_RS17345 (PGN40_17345) holA 3476256..3477254 (-) 999 WP_019391416.1 DNA polymerase III subunit delta -
  PGN40_RS17350 (PGN40_17350) - 3477567..3477701 (+) 135 WP_019391415.1 YqzM family protein -
  PGN40_RS17355 (PGN40_17355) - 3477786..3480086 (-) 2301 WP_272541713.1 DNA internalization-related competence protein ComEC/Rec2 -
  PGN40_RS17360 (PGN40_17360) - 3480150..3480704 (-) 555 WP_019391413.1 ComE operon protein 2 -
  PGN40_RS17365 (PGN40_17365) comEA 3480804..3481418 (-) 615 WP_040056824.1 helix-hairpin-helix domain-containing protein Machinery gene
  PGN40_RS17370 (PGN40_17370) comER 3481508..3482329 (+) 822 WP_120045996.1 late competence protein ComER -
  PGN40_RS17375 (PGN40_17375) - 3482402..3483145 (-) 744 WP_019391410.1 class I SAM-dependent DNA methyltransferase -
  PGN40_RS17380 (PGN40_17380) rsfS 3483145..3483498 (-) 354 WP_019391409.1 ribosome silencing factor -
  PGN40_RS17385 (PGN40_17385) yqeK 3483500..3484066 (-) 567 WP_046217868.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  PGN40_RS17390 (PGN40_17390) - 3484056..3484625 (-) 570 WP_019391407.1 nicotinate-nucleotide adenylyltransferase -
  PGN40_RS17395 (PGN40_17395) yhbY 3484647..3484937 (-) 291 WP_019391406.1 ribosome assembly RNA-binding protein YhbY -
  PGN40_RS17400 (PGN40_17400) aroE 3484959..3485801 (-) 843 WP_019391405.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22356.39 Da        Isoelectric Point: 4.3804

>NTDB_id=774387 PGN40_RS17365 WP_040056824.1 3480804..3481418(-) (comEA) [Priestia filamentosa strain AZC66]
MEWLRTHKWLSAICGGLVCLILLLSIVLGGDKEAKKTATDSESLVFSKEEKNEEEQKKEKAELPIIIDVKGSVQNPGIYT
MKDGERIDDAINKAGGFTKNAEVTAVNLAQKVADEMVVYVPSEGEKELTSIPVEGTDTEESVLININNAQSEELQKLNGV
GPSKAEAIIVYREENGPFKAPEDIMNVSGFGEKSFEKIKEQITV

Nucleotide


Download         Length: 615 bp        

>NTDB_id=774387 PGN40_RS17365 WP_040056824.1 3480804..3481418(-) (comEA) [Priestia filamentosa strain AZC66]
ATGGAATGGTTACGAACCCATAAATGGTTGTCTGCTATTTGTGGAGGTCTTGTTTGTTTGATTCTCTTGTTGTCTATTGT
GTTAGGTGGAGACAAAGAAGCGAAAAAAACAGCAACAGACAGTGAAAGCCTAGTGTTTTCGAAAGAAGAGAAAAATGAAG
AGGAGCAAAAGAAAGAGAAAGCTGAACTTCCGATTATAATTGATGTAAAAGGGAGCGTGCAAAACCCAGGTATCTATACA
ATGAAAGATGGAGAAAGAATTGATGATGCCATTAATAAAGCAGGGGGGTTTACAAAAAATGCAGAAGTAACAGCTGTTAA
TCTAGCTCAAAAAGTAGCTGACGAAATGGTTGTTTACGTTCCTTCTGAAGGTGAGAAAGAATTAACTTCTATTCCTGTTG
AAGGCACTGATACAGAGGAGAGCGTCCTTATTAACATCAACAATGCTCAAAGTGAAGAATTGCAAAAGTTAAACGGTGTG
GGCCCTTCAAAAGCAGAGGCAATTATTGTTTATAGAGAAGAAAATGGTCCATTTAAAGCGCCTGAAGACATTATGAATGT
TAGTGGATTTGGAGAGAAATCATTTGAAAAAATAAAAGAACAAATTACTGTATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-203)

SLBB domain (SLBB)

(67-120)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H4KPF0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

45.794

100

0.48
  comEA/celA/cilE Streptococcus mitis NCTC 12261

38.679

100

0.402
  comEA Latilactobacillus sakei subsp. sakei 23K

32.906

100

0.377