Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H7R76_RS04400 Genome accession   NZ_AP022256
Coordinates   904639..905622 (+) Length   327 a.a.
NCBI ID   WP_182967047.1    Uniprot ID   -
Organism   Klebsiella sp. WP8-S18-ESBL-06     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 899639..910622
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7R76_RS04370 (WP8S18E06_08670) - 900024..901010 (-) 987 WP_182967041.1 TRAP transporter substrate-binding protein -
  H7R76_RS04375 (WP8S18E06_08680) hemW 901315..902451 (-) 1137 WP_182967043.1 radical SAM family heme chaperone HemW -
  H7R76_RS04380 (WP8S18E06_08690) - 902444..903037 (-) 594 WP_182967045.1 XTP/dITP diphosphatase -
  H7R76_RS04385 (WP8S18E06_08700) yggU 903046..903336 (-) 291 WP_106925694.1 DUF167 family protein YggU -
  H7R76_RS04390 (WP8S18E06_08710) - 903333..903899 (-) 567 WP_106925696.1 YggT family protein -
  H7R76_RS04395 (WP8S18E06_08720) - 903918..904622 (-) 705 WP_106925698.1 YggS family pyridoxal phosphate-dependent enzyme -
  H7R76_RS04400 (WP8S18E06_08730) pilT 904639..905622 (+) 984 WP_182967047.1 type IV pilus twitching motility protein PilT Machinery gene
  H7R76_RS04405 (WP8S18E06_08740) - 906153..906806 (+) 654 WP_106925702.1 LuxR C-terminal-related transcriptional regulator -
  H7R76_RS04410 (WP8S18E06_08750) ruvX 906865..907281 (-) 417 WP_064542651.1 Holliday junction resolvase RuvX -
  H7R76_RS04415 (WP8S18E06_08760) - 907281..907844 (-) 564 WP_106925704.1 YqgE/AlgH family protein -
  H7R76_RS04420 (WP8S18E06_08770) gshB 907991..908938 (-) 948 WP_106925706.1 glutathione synthase -
  H7R76_RS04425 (WP8S18E06_08780) rsmE 908951..909682 (-) 732 WP_182967049.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  H7R76_RS04430 (WP8S18E06_08790) endA 909764..910471 (-) 708 WP_182930044.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 327 a.a.        Molecular weight: 35568.81 Da        Isoelectric Point: 6.8604

>NTDB_id=77436 H7R76_RS04400 WP_182967047.1 904639..905622(+) (pilT) [Klebsiella sp. WP8-S18-ESBL-06]
MDIQEIVTLSVKHNVSDLHLCSSGNARWRRAGKLEIAPFSAPHLGVLMDGWLSESQQQTWLRQRQLDFSLSLTGCPRLRA
SAFAHSGGVSLVLRILPTRCPTLDELHPPVALAEMLQEESGLILVTGATGSGKSTTLAAMVDYLNHHLAGHILTLEDPVE
FLHTSEGCLIHQREVGEHAASFADALRVALRRDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDV
FPAQEKEPVRNQLASSLCAVLAQKLQPDNRGGRVALFEMLINTPAVGNLIREGKTHQIAGVMQTGLQSGMQTFSQSYQQR
VKAGMLS

Nucleotide


Download         Length: 984 bp        

>NTDB_id=77436 H7R76_RS04400 WP_182967047.1 904639..905622(+) (pilT) [Klebsiella sp. WP8-S18-ESBL-06]
ATGGATATACAAGAAATCGTGACGCTTAGTGTAAAGCATAACGTGTCCGATCTACACCTATGTAGCAGTGGAAACGCGCG
CTGGCGTAGGGCGGGTAAGCTGGAAATAGCGCCGTTTAGCGCCCCGCATCTCGGCGTGCTAATGGACGGCTGGCTAAGTG
AAAGCCAGCAACAGACGTGGCTGCGGCAGCGACAGCTTGATTTTTCACTGTCGCTGACCGGCTGCCCGCGCCTGCGTGCC
AGTGCGTTTGCCCACAGCGGCGGTGTCTCGCTGGTGCTGCGCATACTGCCGACGCGCTGCCCTACGCTTGACGAACTGCA
TCCGCCGGTGGCGTTAGCGGAGATGCTACAGGAGGAGAGCGGGCTGATTCTGGTGACCGGGGCGACGGGCAGCGGAAAAT
CCACCACGCTGGCGGCGATGGTGGATTACCTTAATCATCACCTGGCCGGGCATATTTTGACGCTGGAAGACCCGGTTGAG
TTTCTGCATACCAGCGAGGGGTGTCTGATTCACCAGCGCGAGGTCGGTGAGCACGCCGCGTCTTTTGCCGATGCGCTGCG
AGTCGCACTACGTCGGGATCCGGATGTGATTCTGCTGGGTGAGCTGCGCGATAGCGAAACCATTCGTCTGGCGCTGACGG
CGGCGGAAACCGGGCACCTGGTGCTGGCAACGCTGCATACGCGCGGCGCGGCACAGGCCATAGAGCGGCTGGTGGACGTG
TTCCCCGCACAGGAAAAAGAGCCCGTGCGCAATCAGCTGGCCAGCAGCCTCTGCGCGGTGCTGGCGCAAAAGCTGCAGCC
GGACAATCGCGGCGGGCGCGTCGCGCTGTTTGAAATGCTGATTAACACGCCGGCGGTGGGTAATCTCATTCGCGAAGGGA
AAACGCACCAGATAGCAGGCGTGATGCAGACCGGGTTGCAAAGCGGCATGCAGACCTTCAGCCAAAGCTATCAACAGCGA
GTTAAGGCGGGGATGTTATCGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.697

100

0.502
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.697

100

0.502
  pilT Neisseria meningitidis 8013

48.338

100

0.489
  pilT Neisseria gonorrhoeae MS11

48.036

100

0.486
  pilT Pseudomonas aeruginosa PAK

46.364

100

0.468
  pilT Pseudomonas stutzeri DSM 10701

46.061

100

0.465
  pilT Legionella pneumophila strain Lp02

46.061

100

0.465
  pilT Legionella pneumophila strain ERS1305867

46.061

100

0.465
  pilT Acinetobacter baumannii D1279779

45.758

100

0.462
  pilT Acinetobacter nosocomialis M2

45.758

100

0.462
  pilT Acinetobacter baylyi ADP1

45.758

100

0.462
  pilT Acinetobacter baumannii strain A118

45.758

100

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.319

96.942

0.459
  pilU Acinetobacter baylyi ADP1

39.938

98.777

0.394
  pilU Pseudomonas stutzeri DSM 10701

40

97.859

0.391
  pilU Vibrio cholerae strain A1552

39.198

99.083

0.388

Multiple sequence alignment