Detailed information NTG0774186

Bioinformatically predicted

Overview

• Name: braS
• Type: Regulator
• Locus tag: PCM89_RS13660
• Genome accession: NZ_CP115887
• Coordinates: 2732159..2733046 (-)
• Length: 295 a.a.
• NCBI ID: WP_000143420.1
• Uniprot ID: A0A2S6DP21
• Organism: Staphylococcus aureus strain C308
• Function: promote expression of competence genes (predicted from homology) Competence regulation

Genomic Context

Location: 2727159..2738046

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCM89_RS13630 (PCM89_13630)	-	2727177..2727265 (+)	89	Protein_2651	hypothetical protein	-
PCM89_RS13635 (PCM89_13635)	-	2727373..2727870 (-)	498	WP_001065267.1	glutathione peroxidase	-
PCM89_RS13640 (PCM89_13640)	-	2727880..2728140 (-)	261	WP_072434822.1	hypothetical protein	-
PCM89_RS13645 (PCM89_13645)	-	2728522..2728635 (-)	114	WP_072384844.1	hypothetical protein	-
PCM89_RS13650 (PCM89_13650)	-	2729299..2731299 (-)	2001	WP_238612126.1	ABC transporter permease	-
PCM89_RS13655 (PCM89_13655)	-	2731296..2732051 (-)	756	WP_000923771.1	ABC transporter ATP-binding protein	-
PCM89_RS13660 (PCM89_13660)	braS	2732159..2733046 (-)	888	WP_000143420.1	nisin susceptibility-associated two-component system sensor histidine kinase NsaS	Regulator
PCM89_RS13665 (PCM89_13665)	braR	2733057..2733722 (-)	666	WP_000697883.1	nisin susceptibility-associated two-component system response regulator NsaR	Regulator
PCM89_RS13670 (PCM89_13670)	-	2733748..2733948 (-)	201	WP_000604723.1	hypothetical protein	-
PCM89_RS13675 (PCM89_13675)	-	2734146..2735570 (+)	1425	WP_000953731.1	alkaline phosphatase	-
PCM89_RS14190	-	2735630..2735731 (-)	102	WP_000731972.1	SE2200 family small protein	-
PCM89_RS13685 (PCM89_13685)	-	2735853..2736308 (-)	456	WP_000106184.1	MarR family winged helix-turn-helix transcriptional regulator	-
PCM89_RS13690 (PCM89_13690)	-	2736554..2737315 (-)	762	WP_000323451.1	alpha/beta hydrolase	-

Sequence

Protein

• Length: 295 a.a.
• Molecular weight: 34045.32 Da
• Isoelectric Point: 5.8962

>NTDB_id=774186 PCM89_RS13660 WP_000143420.1 2732159..2733046(-) (braS) [Staphylococcus aureus strain C308]
MTFLKSITQEIAIVIVIFALFGLMFYLYHLPLEAYLLALGVILLLLLIFIGIKYLSFVKTISQQQQIENLENALYQLKNE
QIEYKNDVESYFLTWVHQMKTPITAAQLLLERDEPNVVNRVRQEVIQIDNYTSLALSYLKLLNETSDISVTKISINNIIR
PIIMKYSIQFIDQKTKIHYEPCHHEVLTDVRWTSLMIEQLINNALKYARGKDIWIEFDEQSNQLYVKDNGIGISEADLPK
IFDKGYSGYNGQRQSNSSGIGLFIVKQISTHTNHPVSVVSKQNEGTTFTIQFPDE

Nucleotide

• Length: 888 bp

>NTDB_id=774186 PCM89_RS13660 WP_000143420.1 2732159..2733046(-) (braS) [Staphylococcus aureus strain C308]
ATGACCTTTCTTAAAAGTATTACTCAGGAAATAGCAATAGTCATAGTTATTTTTGCTTTATTTGGCTTAATGTTTTACCT
GTATCATTTGCCATTAGAAGCATATTTACTAGCACTTGGCGTTATTTTATTATTATTACTCATATTCATAGGTATTAAAT
ATTTAAGTTTTGTAAAAACTATAAGCCAACAACAACAAATTGAAAACTTAGAAAATGCGTTGTATCAGCTTAAAAATGAA
CAAATTGAATATAAAAATGATGTAGAGAGCTACTTTTTAACATGGGTACATCAAATGAAAACACCCATTACTGCAGCACA
ACTGTTACTTGAAAGAGATGAGCCTAATGTTGTGAATCGTGTTCGTCAAGAGGTTATTCAAATTGATAACTATACAAGTT
TAGCACTTAGTTATTTAAAGTTATTAAATGAAACTTCTGATATTTCTGTCACTAAAATTTCGATTAACAATATCATTCGC
CCAATTATTATGAAATATTCAATACAGTTTATTGATCAAAAAACAAAAATCCATTATGAACCTTGTCATCACGAAGTATT
AACTGACGTTAGATGGACCTCTTTAATGATAGAACAATTAATAAATAATGCACTTAAGTATGCGAGAGGTAAAGATATAT
GGATTGAATTTGATGAGCAATCCAATCAATTATATGTAAAAGATAATGGTATCGGTATTAGTGAGGCGGACTTGCCTAAA
ATATTTGATAAGGGCTATTCAGGTTATAATGGCCAGCGTCAAAGTAACTCAAGTGGGATTGGCTTATTTATCGTAAAACA
AATTTCAACTCACACAAACCATCCTGTTTCAGTCGTATCTAAACAAAATGAGGGTACAACATTTACGATTCAATTTCCAG
ATGAATAA

Domains

Predicted by InterproScan.

• Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c) (191-294)

3D structure

• AlphaFold DB: A0A2S6DP21

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
braS	Staphylococcus aureus N315	99.322	100	0.993