Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   PCM89_RS02460 Genome accession   NZ_CP115887
Coordinates   536024..537388 (+) Length   454 a.a.
NCBI ID   WP_001085218.1    Uniprot ID   A0AAE3D3F7
Organism   Staphylococcus aureus strain C308     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 531024..542388
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCM89_RS02440 (PCM89_02440) - 531026..531487 (+) 462 WP_000551762.1 CtsR family transcriptional regulator -
  PCM89_RS02445 (PCM89_02445) - 531506..532072 (+) 567 WP_000882069.1 UvrB/UvrC motif-containing protein -
  PCM89_RS02450 (PCM89_02450) - 532062..533069 (+) 1008 WP_001805606.1 protein arginine kinase -
  PCM89_RS02455 (PCM89_02455) - 533083..535539 (+) 2457 WP_000897132.1 ATP-dependent Clp protease ATP-binding subunit -
  PCM89_RS02460 (PCM89_02460) radA 536024..537388 (+) 1365 WP_001085218.1 DNA repair protein RadA Machinery gene
  PCM89_RS02465 (PCM89_02465) - 537413..538486 (+) 1074 WP_001028376.1 PIN/TRAM domain-containing protein -
  PCM89_RS02470 (PCM89_02470) gltX 539043..540497 (+) 1455 WP_001283794.1 glutamate--tRNA ligase -
  PCM89_RS02475 (PCM89_02475) cysE 540920..541567 (+) 648 WP_001805237.1 serine O-acetyltransferase -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49836.30 Da        Isoelectric Point: 6.6608

>NTDB_id=774151 PCM89_RS02460 WP_001085218.1 536024..537388(+) (radA) [Staphylococcus aureus strain C308]
MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPKHGVKTKELAGKVQKLNSIKHETTPRVLTDSAEFNRV
LGGGIVSGSLVLIGGDPGIGKSTLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGDE
HHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNPSEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPR
RMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDEAVDGLDCYIGEVGLTGEVR
RVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEALSFALHS

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=774151 PCM89_RS02460 WP_001085218.1 536024..537388(+) (radA) [Staphylococcus aureus strain C308]
TTGGCCAAGAAAAAAGTGATTTTTGAATGTATGGCTTGTGGTTATCAATCTCCTAAATGGATGGGGAAATGTCCTAATTG
TGGCGCTTGGAATCAAATGGAGGAAATTGTTGAAAAAGCAGCCAATCCTAAACATGGAGTGAAAACCAAGGAATTAGCAG
GTAAAGTACAAAAATTAAATAGTATTAAACATGAAACAACGCCGAGAGTGTTAACAGATTCAGCAGAATTCAACCGCGTA
TTAGGTGGAGGTATTGTGAGCGGATCGTTAGTACTTATTGGTGGGGATCCAGGTATTGGTAAGTCAACGTTACTTTTACA
AATTTGTGCATCGTTATCTCAAAAGAAAAAAGTACTATATATTACTGGAGAAGAATCGCTTAGTCAGACTAAATTACGTG
CAGAGCGATTAGATGAAGATTCAAGTGAATTGCAAGTATTAGCTGAAACAGATCTTGAAGTTATTTATCAAACAGTAAAA
GAAGAACAACCTGATTTACTAGTAGTGGATTCGATTCAAACAATATATCATCCTGAAATCAGCTCTGCGCCAGGTTCTGT
TTCACAAGTTCGTGAAAGTACACAAAGTTTAATGAATATTGCTAAACAAATGAACATTGCAACTTTTATAGTGGGTCATG
TAACGAAAGAAGGTCAAATTGCTGGCCCAAGATTGCTAGAACACATGGTTGATACTGTGCTTTATTTTGAAGGCGATGAA
CACCACGCATATCGAATTTTGCGAGCTGTTAAAAACCGTTTTGGTTCAACGAATGAAATGGGAATCTTCGAAATGAAGCA
AAGTGGATTAAAAGGTGTAAATAATCCATCTGAAATGTTTTTAGAAGAACGTTCAACAAATGTTCCAGGTTCAACAATTG
TTGCAACCATGGAGGGAACCAGACCACTTTTAATAGAAGTTCAAGCGCTGGTAACTCCAACGACTTTTAACAATCCGAGA
CGAATGGCAACAGGGATTGATCATAATCGATTAAGTTTGTTGATGGCTGTTTTGGAAAAGAAAGAAAATTATCTATTACA
ACAACAAGATGCTTATATCAAAGTAGCTGGCGGTGTAAAGTTAACGGAGCCAGCAGTTGATTTAAGTGTAATTGTAGCAA
CTGCGTCTAGCTTTAAAGATGAAGCTGTCGACGGATTAGATTGCTATATTGGAGAAGTTGGTTTAACGGGTGAGGTACGT
CGTGTATCTCGGATAGAACAACGCGTGCAAGAGGCTGCAAAACTAGGTTTCAAACGTGTAATTATTCCTAAAAATAATAT
AGGCGGATGGACATATCCTGAAGGTATACAAGTAATAGGTGTAACTACTGTACATGAAGCATTGTCATTTGCTCTTCATT
CATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(354-426)

AAA domain (AAA_25)

(77-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.667

100

0.674
  radA Streptococcus pneumoniae Rx1

61.83

98.678

0.61
  radA Streptococcus pneumoniae D39

61.83

98.678

0.61
  radA Streptococcus pneumoniae R6

61.83

98.678

0.61
  radA Streptococcus pneumoniae TIGR4

61.83

98.678

0.61
  radA Streptococcus mitis NCTC 12261

61.83

98.678

0.61
  radA Streptococcus mitis SK321

61.83

98.678

0.61